Unraveling the genetic diversity held in genebanks on a large scale is underway, due to advances in Next-generation sequence (NGS) based technologies that produce high-density genetic markers for a large number of samples at low cost. Genebank users should be in a position to identify and select germplasm from the global genepool based on a combination of passport, genotypic and phenotypic data. To facilitate this, a new generation of information systems is being designed to efficiently handle data and link it with other external resources such as genome or breeding databases. The Musa Germplasm Information System (MGIS), the database for global ex situ-held banana genetic resources, has been developed to address those needs in a user-friendly way. In developing MGIS, we selected a generic database schema (Chado), the robust content management system Drupal for the user interface, and Tripal, a set of Drupal modules which links the Chado schema to Drupal. MGIS allows germplasm collection examination, accession browsing, advanced search functions, and germplasm orders. Additionally, we developed unique graphical interfaces to compare accessions and to explore them based on their taxonomic information. Accession-based data has been enriched with publications, genotyping studies and associated genotyping datasets reporting on germplasm use. Finally, an interoperability layer has been implemented to facilitate the link with complementary databases like the Banana Genome Hub and the MusaBase breeding database. Database URL: https://www.crop-diversity.org/mgis/

The positive and reliable effect of temporary immersion systems on in vitro shoot proliferation was already proved for different plant genera and it is now presented as an alternative for plantain micropropagation. Some culture parameters affecting the efficiency of the twin flasks system or temporary immersion bioreactor (Escalona et al., 1999) were investigated. Three different cytokinins (benzyladenine, thidiazuron and meta-topolin) were added to the culture medium and meta-topolin at a concentration of 4.4 lM was proved to be the most efficient. Successive subcultures (28 days per subculture) were performed on medium supplemented with meta-topolin, revealing a decrease in multiplication after the 6th subculture. Multiplication rate was not changed within the ranges of immersion times (4, 12 or 22 min) and frequencies (every 3, 5 or 7 h) tested. The size of the bioreactor (250, 1,000, 5,000 or 10,000 ml) and the volume of medium per inoculum (10, 20 or 30 ml) were also evaluated and appeared to have an influence on the multiplication. A proportion of 25-100 ml of headspace per inoculum and 30 ml of medium per inoculum resulted in a multiplication rate >13 in 28 days.Abbreviations: BA -N 6 -benzyladenine; MET -meta-topolin; SP medium -standard proliferation medium; TDZ -thidiazuron; TIB -temporary immersion bioreactor

In‐stream surface storage zones (SSZs) caused by lateral recirculation areas play a significant role in the transport and fate of contaminants in rivers. Lateral recirculating areas have long residence times that favor nutrient uptake, accumulation of pollutants, and interactions with reactive sediments. In watersheds affected by acid‐mine drainage, SSZs have profound effects on biogeochemical processes, controlling the local concentration and distribution of toxic elements along the channel. Despite the importance of turbulent flow dynamics on these processes, limited work has been carried out to analyze mass transport in natural SSZs with complex geometries. In this investigation we study a SSZ in the Lluta River, located in a high‐altitude environment in northern Chile, by coupling field measurements and 3‐D numerical simulations to understand the transport mechanisms with the main channel. We measure the velocity field using an acoustic Doppler velocimeter (ADV) and large‐scale particle image velocimetry (LSPIV), extracting the bathymetry from digital image processing. Using these data, we perform detached‐eddy simulations (DES) to analyze the mean flow, turbulence statistics, and the dynamics of large‐scale coherent structures. From this detailed description of the turbulent flow, we study the mass exchange and the time evolution of the mean concentration of a passive scalar in the SSZ by testing three upscaled models: a classical linear transport model, a two‐storage formulation, and a fractional transport model. The analysis integrates temporal and spatial scales to provide a new perspective on the turbulent flow in SSZs and their effects on global mass transport in rivers.

Dysregulation of microRNA (miRNA) expression has been linked to many human diseases; however, because of the challenges associated with RNA-targeted drug discovery, additional approaches are needed for probing miRNA biology. The emerging regulatory role of miRNA-binding proteins in miRNA maturation presents such an alternative strategy. Exploiting our laboratory’s click chemistry-based high-throughput screening (HTS) technology, catalytic enzyme-linked click chemistry assay or cat-ELCCA, we have designed a modular method by which to discover new chemical tools for manipulating pre-miRNA–miRNA–binding protein interactions. Using the pre-let-7d–Lin28 interaction as proof-of-concept, the results presented demonstrate how HTS using cat-ELCCA can enable the discovery of small molecules targeting RNA–protein interactions.

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The positive effect of ventilation of the culture container on in vitro shoot proliferation and quality was already proven for different species. Hereafter we report on the evolution of the headspace during in vitro culture of plantain in a Temporary Immersion Bioreactor (TIB) on the one hand, and culture on semi-solid medium on the other hand. The CO 2 and C 2 H 4 concentration reached a maximum of 12% and 0.45 ll l )1 , respectively in the control treatment on semi-solid medium, compared to 5.7% CO 2 and 0.06 ll l )1 C 2 H 4 in TIB. The minimal O 2 -concentration on semi-solid medium was 15.1%, compared to 19.3% in TIB. The multiplication rate was best in TIB, 6.4 compared to 4.3 in semi-solid conditions, and this was also the case for shoot height (4.3 cm compared to 3.3 cm), and leaf number (2.6 compared to 1.6). Moreover shoots produced on semi-solid medium showed distorted leaves. A typical day-night pattern in CO 2 and O 2 concentration was observed in TIB, as well as on semi-solid medium; this is illustrative for the photosynthetic capacity of the plant material produced in both systems.

In a high-throughput screening campaign, we recently discovered the rRNA-binding tetracyclines, methacycline and meclocycline, as inhibitors of Dicer-mediated processing of microRNAs. Herein, we describe our biophysical and biochemical characterization of these compounds. Interestingly, although direct, albeit weak, binding to the pre-microRNA hairpins was observed, the inhibitory activity of these compounds was not due to RNA binding. Through additional biochemical and chemical studies, we revealed that metal chelation likely plays a principle role in their mechanism of inhibition. By exploring the activity of other known RNA-binding scaffolds, we identified additional disconnections between direct RNA interaction and inhibition of Dicer processing. Thus, the results presented within provide a valuable case study in the complexities of targeting RNA with small molecules, particularly with weak binding and potentially promiscuous scaffolds.