Photosynthesis and carbon metabolism in plantain (Musa AAB) plantlets growing in temporary immersion bioreactors and during ex vitro acclimatization

Aragón, C.; Escalona, Maritza; Capote, Iris; Pina, Danilo; Cejas, Inaudis; Rodrı́guez, Roberto; Cañal, María Jesús; Sandoval, Jorge; Roels, Sophie; Debergh, Pierre; González-Olmedo, J. L.

doi:10.1079/ivp2005640

Cited by 39 publications

(35 citation statements)

References 23 publications

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“…Furthermore, decreased PEPC activity per TSP caused by in vitro conditions was observed in both genotypes. Similar results have been observed in plantain (Aragón et al, 2005). Ex vitro P/R ratios for sea oats averaged 3.6, which was comparable to the P/R ratio we obtained with maize.…”

supporting

confidence: 91%

“…Rubisco activity in vitro has been reportedly reduced by medium supplementation with exogenous sucrose in C 3 species, such as strawberry (Hdider, 1994;Desjardins, 1995) and plantain (Musa AAB cv. CEMSA 3/4) (Aragón et al, 2005). This may, in turn, cause feedback inhibition of photosynthesis because of low phosphate or high sugar-phosphate concentration in the cytosol, both known to affect the regeneration of the rubisco substrate RuBP.…”

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confidence: 99%

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Photosynthetic and carbohydrate status of easy-and difficult-to-acclimatize sea oats (Uniola paniculata L.) genotypes during In vitro culture and Ex vitro acclimatization

Valero-Aracama

Kane

Wilson

et al. 2006

In Vitro Cell. Dev. Biol. - Plant

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SummaryThe photosynthetic and carbohydrate status of an easy-to-acclimatize (EK 16-3) and a difficult-to-acclimatize (EK 11-1) genotype of Uniola paniculata L. (sea oats), a native dune species of the southeastern US, were evaluated during in vitro culture and ex vitro acclimatization. Net photosynthetic rate was eight times greater for EK 16-3 than EK 11-1 plantlets after ex vitro transfer. In vitro-produced leaves were morphologically similar to ex vitro-produced leaves and exhibited similar photosynthetic competence. EK 11-1 plantlets exhibited greater transpiration rates at the time of ex vitro transfer than EK 16-3 plantlets. However, the small magnitude of this difference, although significant, indicated that control of water loss was probably not the main cause for poor acclimatization of EK 11-1 plantlets. Carbohydrate analysis in vitro revealed that EK 16-3 plantlets utilized leaf starch reserves more rapidly than EK 11-1 plantlets. Starch utilization correlated with the development of leaves with expanded leaf blades during in vitro rooting in EK 16-3 plantlets. After ex vitro transfer, both genotypes exhibited significant decreases of starch and soluble sugar content in shoots and roots. However, the higher photosynthetic ability of shoots in EK 16-3 resulted in greater accumulation of shoot soluble sugars than EK 11-1 after 2-wk ex vitro culture. After 6-wk in vitro rooting, there were significantly higher chlorophyll and soluble protein contents, ribulose 1,5-bisphosphate carboxylase (rubisco) and phosphoenolpyruvate carboxylase activities in EK 16-3 than EK 11-1 shoots. These differences also correlated with the development of anatomical and morphological leaf features in EK 16-3 similar to those of greenhouse-produced leaves.

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supporting

confidence: 91%

mentioning

confidence: 99%

Photosynthetic and carbohydrate status of easy-and difficult-to-acclimatize sea oats (Uniola paniculata L.) genotypes during In vitro culture and Ex vitro acclimatization

Valero-Aracama

Kane

Wilson

et al. 2006

In Vitro Cell. Dev. Biol. - Plant

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“…Larger leaves in TIS have been obtained with Ananas comosus (pineapple) and chrysanthemum (Escalona et al 2003;Hahn and Paek 2005). Leaves can function as storage organs during micropropagation, and the reserves can be used during the transition to autotrophic conditions during the rooting and acclimatization phases (Aragón et al 2005). Thus, the larger leaves obtained in TIS may help in the transition to soil conditions.…”

Section: Resultsmentioning

confidence: 99%

A temporary immersion system for micropropagation of axillary shoots of hybrid chestnut

Vidal

Blanco

Cuenca

2015

Plant Cell Tiss Organ Cult

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A protocol for culturing chestnut axillary shoots by temporary immersion in liquid medium was developed. The influence of type of explant, support material, bioreactor, and immersion was investigated for five artificial hybrids and five natural hybrids of Asian and European chestnut selected for resistance to ink disease. The type of explant influenced shoot quality and proliferation rates, and basal explants with callus produced more and longer shoots than apical and nodal segments. Use of rockwool cubes as support material prevented hyperhydricity and allowed proliferation of explants in Murashige and Skoog medium with half-strength nitrates supplemented with 0.22 lM BA and 3 % sucrose, cultured both in plantform TM and RITA Ò vessels with three or six immersions per day and additional aeration of 1 min per hour in the case of plantform TM bioreactors. Basal explants cultured in plantform TM for 5 weeks produced long shoots suitable for rooting, whereas apical and nodal explants cultured in plantform TM or RITA Ò produced shorter shoots that were suitable for maintenance of stock. For most of the clones, similar or higher proliferation rates were observed when cultured in liquid medium than in semisolid medium, with the additional benefit of cost-reduction of the former system. Shoots developed in liquid medium were submitted to ex vitro root induction by dipping in indole-3-butyric acid, and acclimatized under greenhouse conditions. This is the first demonstration of the production of chestnut plantlets from shoots cultured in liquid medium, and the protocol presented here shows good potential for application in large-scale propagation.

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“…Micropropagated plants from TIS soon increase photosynthesis, number of leaves and roots, and dry weight during ex vitro acclimatization (Rodríguze et al 2003;Aragón et al 2005). Micropropagated Calathea plants obtained from conventional semi-solid medium were susceptible to water stress after transfer (Ebrahim and Ibrahim 2000).…”

Section: Introductionmentioning

confidence: 99%

In vitro leaf anatomy, ex vitro photosynthetic behaviors and growth of Calathea orbifolia (Linden) Kennedy plants obtained from semi-solid medium and temporary immersion systems

Yang

Yeh

2008

Plant Cell Tiss Organ Cult

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In vitro bud clusters of Calathea orbifolia (Linden) Kennedy were obtained and subcultured in semi-solid (agar) medium and temporary immersion system (TIS) for 12 weeks. Uniform young plants were selected and transferred to soilless mix in a growth chamber for ex vitro acclimatization during 35 days, followed by growing in a shaded greenhouse for 65 days. Comparison of in vitro leaf anatomy, ex vitro photosynthetic behaviors and growth was made between two cultural systems. Plants in TIS produced thicker leaf chlorenchyma and aquiferous parenchyma, lower stomatal frequency and more epicuticular wax than did those in semi-solid medium. Plants from semi-solid medium had consistently lower leaf Fv/Fm values than plants from TIS. Leaf Fv/Fm value in plants from TIS decreased to 0.65 at day 7 after transfer and increased soon up to 0.76 thereafter. In contrast, leaf Fv/Fm value in plants from semi-solid medium reduced to 0.27 at day 7 after transfer and increased slowly up to 0.68 at day 35. During ex vitro acclimatization, plants in TIS had substantial higher photosynthetic rates than plants in semi-solid medium. Plants from TIS had subsequent higher leaf area, fresh and dry weights than plants from semi-solid medium.

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Photosynthesis and carbon metabolism in plantain (Musa AAB) plantlets growing in temporary immersion bioreactors and during ex vitro acclimatization

Cited by 39 publications

References 23 publications

Photosynthetic and carbohydrate status of easy-and difficult-to-acclimatize sea oats (Uniola paniculata L.) genotypes during In vitro culture and Ex vitro acclimatization

Photosynthetic and carbohydrate status of easy-and difficult-to-acclimatize sea oats (Uniola paniculata L.) genotypes during In vitro culture and Ex vitro acclimatization

A temporary immersion system for micropropagation of axillary shoots of hybrid chestnut

In vitro leaf anatomy, ex vitro photosynthetic behaviors and growth of Calathea orbifolia (Linden) Kennedy plants obtained from semi-solid medium and temporary immersion systems

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