Optimization of plantain (Musa AAB) micropropagation by temporary immersion system

Roels, Sophie; Escalona, Maritza; Cejas, Inaudis; Noceda, Carlos; Rodrı́guez, Roberto; Cañal, M.J.; Sandoval, Jorge; Debergh, Pierre

doi:10.1007/s11240-004-6746-y

Cited by 97 publications

(70 citation statements)

References 16 publications

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“…Por su parte, mediante el uso de este sistema, McAlisteret al (2005) reprodujeron distintos clones de Eucalyptus spp., duplicando la tasa de multiplicación en la mitad del tiempo, comparado con el método tradicional en medio semisólido. En este contexto, la tasa de multiplicación está influenciada por el tipo y concentración de citoquinina utilizada, como es el caso reportado por Roels et al (2005) en Musa spp.…”

Section: Introductionunclassified

Multiplicación in vitro de Eucalyptus globulus mediante sistema de inmersión temporal

González¹,

Ríos²,

Avilés³

et al. 2011

Bosque (Valdivia)

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SUMMARYWith the object of establishing mass production methods of Eucalyptus globulus, factors affecting its in vitro multiplication stage by temporary immersion were studied. For this, five microshoots per flask containing 250 mL of Murashige and Skoog medium were disposed. Factors such as immersion frequency (6, 12, 24, 48 h), immersion time (1, 2 and 3 min) and macronutrients concentration (25, 50 and 100 %) were studied. In a second stage, the addition into medium of 6-bencylaminopurine (0, 2.2 and 4.4 µM), polyvinylpyrrolidone (0, 250 y 500 mg L -1 ) and sucrose concentration (43.8 and 87.6 mM) was analyzed. A completely random design with factorial arrangement was used in each stage. Variables evaluated were number of new shoots per flask, dry and fresh weight rate and final pH of culture medium. The best rate of multiplication and less hyperhydration was obtained with immersion time of 2 min, a frequency of 12hours, macronutrients at 50 %, 2.2 µmol of 6-bencylaminopurine, 43.8 mM of sucrose and 250 mg L -1 of polyvinylpyrrolidone. Time and frequency of immersion interaction affects multiplication rate and microshoot hyperhydration. Use of 6-bencylaminopurine (2,2 µM) and polyvinylpyrrolidone (250 mg L -1 ) increases multiplication rate without influence on microshoot hyperhydration. A reduction on sucrose concentration decreases hyperhydration without affecting formation of new shoots.

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Section: Introductionunclassified

Multiplicación in vitro de Eucalyptus globulus mediante sistema de inmersión temporal

González¹,

Ríos²,

Avilés³

et al. 2011

Bosque (Valdivia)

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“…Um dos principais aspectos que limitam a expansão da bananicultura no Brasil é a utilização de mudas produzidas por meio de métodos convencionais que, além de apresentarem baixa taxa de multiplicação, podem-se constituir num mecanismo de disseminação de pragas e doenças (Roels et al, 2005), ocasionando perdas que podem alcançar 100% na produtividade, no caso da sigatoka-negra (Silva et al, 2003).…”

Section: Introductionunclassified

Micropropagação da bananeira 'Maçã', cultivada in vitro em diferentes volumes de meio líquido

Siqueira¹,

Santos²,

Salomão³

et al. 2013

Rev. Ceres

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Micropropagation of 'Maçã' banana tree, cultivated in vitro in different volumes of liquid mediumThis study aimed to evaluate the effect of different volumes of stationary MS liquid medium on the in vitro multiplication rate and development of banana 'Maçã' explants. During the multiplication phase, were used microrhizome of the cultivar 'Maçã' (AAB), derived from pre-established plants on solid culture medium, which were standardized for size. The experimental design was a completely randomized with seven treatments and ten replicates, and the plot consisted of four explants. The treatments were different volumes of the liquid stationary medium (5, 10, 15, 20, 25, 30 mL) and 30 mL of semi-solid medium (standard). Microplants produced at the end of the third subculture were transferred to a growth and rooting medium. The experiment consisted of the same treatments and design, and each plot comprises four explants. The different liquid volume and the semi-solid did not alter the explants multiplication rate. It is possible to produce microplants of banana 'Maçã' in vitro in liquid stationary medium, being the optimal volume of the 25 mL per flask.

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“…A utilização de mudas convencionais, por exemplo mudas de rizoma, contribui significativamente para a disseminação de pragas e doenças (Souza et al, 2000;Roels et al, 2005).…”

Section: Introductionunclassified

Enraizamento in vitro de mudas micropropagadas de bananeira (Musa sp.) em diferentes meios de cultivo

et al. 2010

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RESUMOObjetivou-se, neste trabalho, avaliar o processo de enraizamento in vitro de mudas micropropagadas das cultivares de bananeira (Musa sp.) 'Nanicão Jangada' (AAA), 'Nanicão Grande Naine' (AAA) e 'Maçã' (AAB) utilizando-se os meios de cultivo semissólido e líquido. Foram utilizadas mudas micropropagadas oriundas de meristemas retirados de mudas do tipo chifrinho coletadas em bananais comerciais na Região do Médio Paranapanema, estado de São Paulo. Foram constituídos dois tratamentos sendo: meio líquido, sem a adição de agente gelificante e meio semissólido, gelificado com 7 g L -1 de ágar. O delineamento estatístico utilizado foi o inteiramente casualizado, sendo que cada tratamento foi constituído por 30 frascos, contendo 6 mudas/frasco. Após 30 dias, as plântulas foram retiradas dos frascos e foram avaliados os seguintes parâmetros: comprimento da maior raiz, número total de raízes, número total de folhas e altura da parte aérea. Foram avaliadas 50 plântulas por tratamento. Os resultados obtidos demonstraram que as 3 cultivares avaliadas apresentaram melhor desenvolvimento radicular em meio semissólido, entretanto, o número de raízes foi maior em meio líquido, principalmente para a cultivar 'Maçã'. O número total de folhas por plântula foi maior, de modo geral, no meio semissólido, enquanto que a altura das plântulas foi maior no meio líquido. A cultivar 'Maçã' apresentou as maiores diferenças entre os meios semissólido e líquido em todos os parâmetros avaliados. Termos para indexação:Micropropagação, cultivares, sistema radicular. ABSTRACTThe objective of this study was to evaluate the process of in vitro rooting of micropropagated seedlings of 'Nanicão Jangada' (AAA), 'Nanicão Grande Naine' (AAA) and 'Maçã' (AAB) banana cultivars (Musa sp.) using semi-solid and liquid media. Plantlets originated from micropropagated meristems removed from seedlings collected in banana trade in the Médio Paranapanema region, State of São Paulo were used. Two treatments were tested: liquid medium without the addition of jelling agent and a semi-solid medium with 7 g L -1 agar. The statistical design was completely randomized, each treatment was set for 30 flasks containing 6 seedlings per flask. After 30 days, the seedlings were removed from flask, and the following parameters were assessed: length of the longest root, the total number of roots, the total number of leaves and plant height. Fifty seedlings were evaluated per treatment. The results demonstrate that the 3 cultivars evaluated showed better root development in semi-solid medium, however, the number of roots was higher in liquid medium, mostly for 'Maçã' cultivar. The total number of leaves per seedling was higher, generally in semisolid medium, while plant height was higher in liquid medium. The 'Maçã' cultivar presented the largest differences between the semisolid and liquid media in all parameters evaluated.

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Optimization of plantain (Musa AAB) micropropagation by temporary immersion system

Cited by 97 publications

References 16 publications

Multiplicación in vitro de Eucalyptus globulus mediante sistema de inmersión temporal

Multiplicación in vitro de Eucalyptus globulus mediante sistema de inmersión temporal

Micropropagação da bananeira 'Maçã', cultivada in vitro em diferentes volumes de meio líquido

Enraizamento in vitro de mudas micropropagadas de bananeira (Musa sp.) em diferentes meios de cultivo

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