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Resumo -As principais plantas daninhas encontradas na cultura do trigo são o nabo e o azevém, e seu controle em pós-emergência é baseado em herbicidas inibidores da enzima acetolactato sintase (ALS) e acetil coenzima A carboxilase (ACCase) que, em função da resistência, limita as opções de controle. Associações de herbicidas têm sido utilizadas em pós-emergência do trigo, porém, seus efeitos na cultura são pouco conhecidos. O objetivo deste trabalho foi avaliar a seletividade de associações de herbicidas e doses aplicados em pós-emergência do trigo. Foram realizados três experimentos a campo em sistema de semeadura direta, no delineamento de blocos ao acaso com três repetições. Os experimentos foram conduzidos nos municípios de Santa Bárbara do Sul-RS, Cruz Alta-RS e Tupanciretã-RS. Foram estudadas doses e associações entre os herbicidas 2,4-D, metsulfuron-methyl, iodosulfuron, piroxsulam e saflufenacil aplicados no perfilhamento do trigo. As variáveis avaliadas foram fitotoxicidade aos sete, 14, 21 e 35 dias após a aplicação (DAA), rendimento de grãos (kg ha -1 ) e peso hectolitro dos grãos de trigo (PH), sendo os dados dos três experimentos analisados de forma conjunta. Aos 35 DAA as maiores fitotoxicidades foram observadas para metsulfuron-methyl na dose de 9 g i.a. ha -1 e para saflufenacil associado ao metsulfuron-methyl. Os resultados de rendimento de grãos demonstraram que a associação entre 2,4-D e metsulfuron-methyl, saflufenacil isolado ou associado à metsulfuron-methyl e o iodosulfuron-methyl foram seletivos para o trigo. O aumento da dose de metsulfuron-methyl (6 e 9 g i.a. ha -1 ) e a dose de registro de piroxsulam causaram redução significativa na rendimento de grãos de grãos de trigo na média dos três experimentos. O PH dos grãos de trigo não foi influenciado pelos herbicidas estudados. Palavras-chave: Triticum aestivum, mistura de herbicidas, mistura em tanque, resistência Abstract -The main weeds in wheat are wild radish and ryegrass, and its post-emergence control is based on herbicides inhibiting the enzyme acetolactate synthase (ALS) and acetyl coenzyme A carboxase (ACCase), which as a function of resistance limits the control options. Herbicide combinations have been used for post-emergence of wheat; however, the effects of herbicidal combinations are poorly understood. The objective of this work was to evaluate the selectivity of Piasecki et al. Rev. Bras. Herb., v.16, n.4, p.286-295, out./dez. 2017 287

Conyza bonariensis (hairy fleabane) is one of the most problematic and widespread glyphosate-resistant weeds in the world. This highly competitive weed species significantly interferes with crop growth and substantially decreases crop yield. Despite its agricultural importance, the molecular mechanisms of glyphosate resistance are still unknown. The present RNA-Seq study was performed with the goal of identifying differentially expressed candidate transcripts (genes) related to metabolism-based non-target site glyphosate resistance in C. bonariensis. The whole-transcriptome was de novo assembled from glyphosate-resistant and -sensitive biotypes of C. bonariensis from Southern Brazil. The RNA was extracted from untreated and glyphosate-treated plants at several timepoints up to 288 h after treatment in both biotypes. The transcriptome assembly produced 90,124 contigs with an average length of 777 bp and N50 of 1118 bp. In response to glyphosate treatment, differential gene expression analysis was performed on glyphosate-resistant and -sensitive biotypes. A total of 9622 genes were differentially expressed as a response to glyphosate treatment in both biotypes, 4297 (44.6%) being up- and 5325 (55.4%) down-regulated. The resistant biotype presented 1770 up- and 2333 down-regulated genes while the sensitive biotype had 2335 and 2800 up- and down-regulated genes, respectively. Among them, 974 up- and 1290 down-regulated genes were co-expressed in both biotypes. In the present work, we identified 41 new candidate target genes from five families related to herbicide transport and metabolism: 19 ABC transporters, 10 CYP450s, one glutathione S-transferase (GST), five glycosyltransferases (GT), and six genes related to antioxidant enzyme catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD). The candidate genes may participate in metabolic-based glyphosate resistance via oxidation, conjugation, transport, and degradation, plus antioxidation. One or more of these genes might ‘rescue’ resistant plants from irreversible damage after glyphosate treatment. The 41 target genes we report in the present study may inform further functional genomics studies, including gene editing approaches to elucidate glyphosate-resistance mechanisms in C. bonariensis.

The goals of this study were to evaluate herbicide application rates at different timings for preharvest desiccation of wheat (Trial 1), as well as to evaluate the effect of the timing of herbicide desiccation at preharvest and harvest timing (Trial 2) on yield, germination, and herbicide residue in wheat seed. In Trial 1, treatments consisted of two application rates of glufosinate, glyphosate, paraquat, or paraquat+diuron and a control without application; application time periods were in the milk grain to early dough stage, soft dough to hard dough stage, and hard dough stage. In Trial 2, treatments consisted of different application time periods (milk grain to early dough stage, and soft dough to hard dough stage), different herbicides (glufosinate, 2,4-D+glyphosate, and untreated control), and different harvest times (5, 10 and 15 days after herbicide application). One thousand seeds weight, yield, first and final germination count, and herbicide residue on seeds were evaluated. Preharvest desiccation with paraquat, glufosinate, and 2,4-D+glyphosate at the milk grain to early dough stage reduces wheat yield. Regardless of the herbicide and application rate, application in the milk grain to early dough stage and soft dough to hard dough stage provides greater germination of wheat seeds, except at the lower dose of paraquat. Systemic herbicides accumulate more in wheat seeds.

-The repetitive use of iodosulfuron for the control of weeds in winter cereals in the south of Brazil has favored the emergence of resistant Raphanus sativus biotypes. The objective of this study was to evaluate: the response of Raphanus sativus biotypes susceptible and resistant to different dosages of iodosulfuron; the control of biotypes with alternative registered herbicides for the control of the species in crops of wheat, corn and soybean; and the existence of cross-resistance of the biotypes. Thus, four experiments were done in a greenhouse, with a completely randomized design and four replicates. The experimental units were composed of vases with a volumetric capacity of 0.75 L filled with substrate, containing a plant each. For the dose-response curve, three biotypes (factor A) and nine doses of the iodosulfuron herbicide (factor B) were used. For the alternative control, the recommendation was herbicides in pre or postemergence of the crops, and the crossedresistance was evaluated by using herbicides that inhibit the ALS enzyme of different chemical groups. The analyzed variables were control and shoot dry matter. GR 50 of the susceptible biotype (B 1 ) was 0.11 g a.i. ha -1 , whereas GR 50 of resistant biotypes (B 4 and B 13 ) was 102.9 and 86.8 g a.i. ha -1 of the iodosulfuron herbicide, respectively. The resistant biotypes presented crossed resistance to herbicides that inhibit the ALS enzyme, where the control can be efficient with the use of herbicides with different action mechanisms. Keywords:Raphanus sativus, dose-response, acetolactate synthase, weed. RESUMO -O uso repetido do herbicida iodosulfurom para controle de plantas daninhas em cereais de inverno no Sul do Brasil favoreceu o surgimento de biótipos de Palavras-chave:Raphanus sativus, dose-resposta, acetolactato sintase, planta daninha.

Italian ryegrass (Lolium multiflorum; LOLMU) is one of the most troublesome weeds in temperate regions in the world. This weed species interfere with wheat, corn, rye, and oat, causing significant crop yield losses. This species has evolved glyphosate resistance, making it difficult to control. The mechanisms of glyphosate resistance are still unknown, and an understanding thereof will favor the development of new strategies of management. The present study is the first transcriptome study in LOLMU using glyphosate-resistant and -sensitive biotypes, aiming to identify and to provide a list of the candidate target genes related to glyphosate resistance mechanism. The transcriptome was assembled de novo, producing 87,433 contigs with an N50 of 740 bp and an average length of 575 bp. There were 92 and 54 up- and down-regulated genes, respectively, in the resistant biotype, while a total of 1683 were differentially expressed in the sensitive biotype in response to glyphosate treatment. We selected 14 highly induced genes and seven with repressed expression in the resistant biotype in response to glyphosate. Of these genes, a significant proportion were related to the plasma membrane, indicating that there is a barrier making it difficult for glyphosate to enter the cell.

Radish (Raphanus sativus L.) and wild radish (R. raphanistrum L.) are troublesome agricultural weeds in several areas worldwide, with populations resistant to acetolactate synthase (ALS) inhibitor herbicide. Information on the mechanisms of resistance is important for management and developing novel solutions to control resistant populations. The objective of this work was to determine the ALS‐resistance mechanisms in radish and wild radish biotypes resistant to ALS‐inhibiting herbicides. Resistance levels were determined by dose‐response curve using several rates of metsulfuron‐methyl herbicide in susceptible (JUC1) and resistant (JUC2, CRA3) radish, and susceptible (CAF1) and resistant (CAF2, CAF3) wild radish biotypes. The mechanism was evaluated by the in vitro activity of the ALS enzyme with imazapic herbicide and ALS gene sequencing. All resistant biotypes were insensitive to metsulfuron‐methyl and imazapic herbicides. The rate of metsulfuron‐methyl to reduce 50% growth of the plants (GR50) were 17‐ to 60‐fold (JUC2, CRA3) and 9‐ to 21‐fold (CAF2 and CAF3) higher than the susceptible biotypes of radish and wild radish, respectively. One‐point mutation in the ALS gene, Trp‐574‐Leu, was found in all resistant radish biotypes, whereas a second point mutation, Ala‐360‐Ser, was found in CAF2, CAF3, and JUC2 resistant biotypes. Changes in kinetic parameters of the ALS enzyme were found in all resistant biotypes evaluated, causing reduction of the herbicide affinity in the target‐site of action. Trp‐574‐Leu amino acid substitution resulted in resistance to sulfonylurea, imidazolinone, and triazolopyrimidine groups, with the survival greater than 75% of the plants, depending on the herbicide applied at labeled rate.

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