Oxidative stress and differential antioxidant enzyme activity in glyphosate-resistant and -sensitive hairy fleabane in response to glyphosate treatment

Piasecki, Cristiano; Carvalho, Ivan Ricardo; Cechin, Joanei; Goulart, Francisco; Maia, Luciano Carlos da; Agostinetto, Dirceu; Caverzan, Andréia; Stewart, C. Neal; Várgas, Leandro

doi:10.1590/1678-4499.20180289

Cited by 18 publications

(34 citation statements)

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“…The ROS production after glyphosate treatment has been demonstrated in GR and GS biotypes of hairy fleabane (Piasecki et al, 2019) and the literature indicates that the ROS leads to RNA degradation in plants (Foyer and Noctor, 2005;Gill and Tuteja, 2010). Also, plants of hairy fleabane show damaged aspect at 192 HAT, which is more intense in GS plants than in GR (Figure 1).…”

Section: Resultsmentioning

confidence: 86%

“…Two time-course experiments after glyphosate treatment were performed at Universidade Federal de Pelotas (UFPel) in Pelotas, Rio Grande do Sul State, Brazil. The experiments were carried out using plants of two biotypes of hairy fleabane previously characterized as glyphosateresistant (B11R) and -sensitive (B17S), with a resistance factor of 18.2 (Piasecki et al, 2019). The plants were cultivated in a greenhouse until 60 days after emergence (DAE) at 25 o C/15 o C day/night (± 3 o C) and 12-hours photoperiod.…”

Section: Methodsmentioning

confidence: 99%

“…In E2, the time-course experiment was determined based on E1 to determine glyphosate rates. The evaluation time was determined based on results of another study which indicated that shikimic acid content in B11R treated plants did not differ from untreated at 288 HAT and that B17S died after 192 HAT (Piasecki et al, 2019).…”

Section: Methodsmentioning

confidence: 99%

“…A plausible hypothesis is that RNA-Seq analysis has been performed in a short time after glyphosate treatment, and do not capture all process involved in plant's herbicide detoxification. Studies have shown that shikimicacid accumulation is transient in GR hairy fleabane, reaching the peak at 96 h after glyphosate treatment and taking around 300 h until their complete detoxification (Piasecki et al, 2019). Thus, whether this process is transient, it could be the first indicator that how long time the 3 plant metabolism system takes to deal with the stress generated after glyphosate treatment.…”

Section: Introductionmentioning

confidence: 99%

“…In another hand, the glyphosate treatment leads to the reactive oxygen species (ROS) production (Cobb and Reade, 2010;Piasecki et al, 2019). The ROS are highly reactive toxic molecules which cause several damages to plant cell, including the degradation of nucleic acids (RNA and DNA) (Foyer and Noctor 2005;Gill and Tuteja 2010).…”

Section: Introductionmentioning

confidence: 99%

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Does the Glyphosate Treatment Interfere Negatively on RNA Integrity in Glyphosate-Resistant and -Sensitive Conyza bonariensis?

et al. 2019

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The hairy fleabane (Conyza bonariensis (L.) Cronq.) is among the most problematic glyphosate-resistant weeds to manage around the world. In weed science, molecular approaches such as RNA sequencing (RNA-Seq) and quantitative reverse-transcription polymerase chain reaction (RT-qPCR) have been employed to study molecular responses to glyphosate treatment in Conyza species. Glyphosate treatment leads to reactive oxygen species (ROS) production in plants which could damage the RNA. Degraded RNA is an issue and can compromise further molecular analysis. Thus, the objective of this study was to evaluate whether glyphosate treatment interferes negatively on RNA integrity of glyphosate-resistant andsensitive hairy fleabane biotypes. Two experiments were performed using glyphosate doses from 0 to 11,840 g a.e. ha -1 and evaluated in a time-course until 288 hours after treatment. The total of 86 RNA samples were evaluated. The RNA integrity was evaluated in a Bioanalyzer 2100 equipment according to RNA integrity number (RIN) scores and electrophoresis gel. The RIN scores ranged from 5.1 to 9.0. Glyphosate doses do not reduce the RIN scores in both glyphosate-resistant and -sensitive biotypes of hairy fleabane. Visual and automatic analysis of electrophoresis gel show suitable results for all RNA samples, with well-defined bands at 28S and 18S positions and no degradation. The results of the analysis indicate that glyphosate treatment does not affect the RNA integrity of glyphosate-resistant and -sensitive biotypes of hairy fleabane until 288 and 192 hours after glyphosate treatment, respectively. The RNA integrity analysis provides useful results to evaluate the RNA condition for further analysis. However, the costs were around US$ 14.25 per sample, considering only reagents. These results are useful for planning future timecourse experiments in Conyza spp. after glyphosate treatment.Keywords: hairy fleabane, molecular analysis, RNA integrity number (RIN). (Conyza bonariensis (L.) Cronq.) está entre as plantas daninhas resistentes ao glifosato mais difíceis de serem manejadas em todo o mundo. Na ciência das plantas daninhas, abordagens moleculares, como o sequenciamento de RNA (RNA-Seq) e a reação da transcriptase reversa da polimerase em tempo real (RT-qPCR), têm sido empregadas para estudar as respostas moleculares ao tratamento com glifosato em espécies de Conyza. No entanto, o tratamento com glifosato leva à produção de espécies reativas de oxigênio (ROS) em plantas, que podem danificar o RNA. A degradação do RNA é um problema e pode comprometer futuras análises moleculares. Assim, o objetivo deste estudo foi avaliar se o tratamento com glifosato interfere negativamente na integridade do RNA de biótipos de buva resistente e sensível ao glifosato. Dois experimentos foram realizados I D I D I D Planta Daninha 2019; v37:e019217909 PIASECKI, C. et al. Does the glyphosate treatment interfere negatively on RNA integrity in glyphosate-resistant and -sensitive ... 2 utilizando doses de glifosato de 0 a 11.840 g e.a. ha -1 e av...

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Section: Resultsmentioning

confidence: 86%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%