DNA barcoding based on mitochondrial DNA COI gene is very useful in identifying of Indonesian hornbill. We sequenced the DNA barcode of seven hornbill species using the mitochondrial DNA COI gene to explore their genetic variation, identity, distance, and phylogenetic. Thirty-one blood samples from seven hornbill species were isolated and analyzed. Slight variation was observed within the nucleotide of the hornbill species. In contrary, fairly significant difference was shown within the genus and family level. COI gene sequences generated from this study, showed unmatched result with BoLD System database. These seven Indonesian hornbill species were then divided into two groups, namely Group I consisting of Aceros cassidix, Rhyticeros plicatus, R. undulatus, Buceros rhinoceros, and B. bicornis, while Group II occupied by Anthracoceros albirostris and A. malayanus; both groups with genetic distance 5.90%. Overall in-group had 9.40% distances to the hornbill used as the out group. COI sequence gene from these seven hornbill species are novel for identifying Indonesian hornbills. We encourage its use as quick species identification, applied to prevent illegal poaching conservation management.

Genetic information of Indonesian hornbills (Julang) is still limited. We sequenced the cytochrome b gene of three hornbill species (Rhyticeros undulatus, R. plicatus, and Aceros cassidix) to explore their genetic variation, distance, and phylogenetic. Nine blood samples for Cyt b from three hornbill species were isolated with Dneasy® Blood and Tissue Kit base on Spin-Column Protocol, Qiagen. SNP, genetic distance, and phylogeny were calculated by MEGA 6.0 software. This study was found variations in the Cyt b gene sequence (849 bp) between three species Indonesian Julang. Rhyticeros undulatus had the most SNP compared to R. plicatus and Aceros cassidix. Substitution of synonyms is more common than substitution of synonyms and transition substitution frequency tends to be much higher than transversion substitution. The three species of Indonesian Julang were markedly separate with an average genetic distance of 5.25%. and separated with the outgroup species at a genetic distance of 9.43%. Cytochrome b sequence genes from these three hornbill species are novel for identifying and classifying hornbills, and could be applied to prevent illegal poaching and conservation management in Indonesia.

This study aims to inventory the types of plants used as food and resting place by Macaca fascicularis in the mixed garden area of ​​Chief Curup Village, Rejang Lebong Regency, Bengkulu. The method used is the Continuous-time recording method and focal animal sampling which is focused on four target individuals in the observed group. The results showed that the plants used by M. fascicularis as a food source were Arenga pinnata, Schizostachyum brachycladum, Capsicum frutescens, Durio zibethinus, Archidendron pauciflorum, Terminalia catappa, Garcinia mangostana, Musa paradisiaca, Parkia speciosa, ferns from the order Cyatheales and Hibiscus tiliaceus. While the plants used as resting places were Durio zibethinus, Terminalia catappa and Schizostachyum brachycladum. In conclusion, there are 11 types of plants that are used as food sources by M. fascicularis (fruit, flowers, leaves, petioles), and 6 of them are community cultivated plants. In addition, there are two types of plants that are used as a place to rest during the day and one type of plant as a place to sleep at night. Keywords: Macaca fascicularis, Long Tailed Monkey, Forage Plants

Golongan darah bersifat herediter dan sangat penting untuk diketahui karena terkait dengan keberhasilan transfusi darah, transplantasi organ, maupun penyakit herediter. Pada sistem golongan darah yang sering digunakan adalah sistem ABO, berdasarkan keberadaan antigen A dan B yang terdapat di permukaan sel darah merah. Serta sistem golongan darah rhesus merupakan terbesar kedua setelah sistem ABO, yang ditentukan berdasarkan keberadaan antigen D. Karena pentingnya mengetahui golongan darah dan rhesus sehingga perlu adanya pemeriksaan golongan darah dan rhesus siswa kelas X di SMA Negeri 11 Bengkulu Utara. Metode yang digunakan dalam kegiatan pengabdian ini meliputi persiapan alat bahan yang digunakan untuk pemeriksaan golongan darah serta pelaksanaan kegiatan sehingga didapatkan hasil golongan darah setiap siswa. Pemeriksaan golongan darah diikuti oleh siswa sebanyak 34 orang, 13 laki-laki dan 21 perempuan. Berdasarkan hasil pemeriksaan, persentase siswa yang memiliki golongan darah A 29,4%, golongan darah B sebanyak 29,4%, golongan darah O sebanyak 29,4%, serta golongan darah AB yang paling sedikit yaitu 11,8%. Berdasarkan hasil pemeriksaan rhesus, menunjukkan bahwa semua siswa memiliki rhesus positif (Rh+).

Guild composition and niche breadth are important point on avian studies. This paper describes the guild composition and niche breadth of understorey bird in Sipisang, West Sumatra. The study was conducted since May 2002 until October 2004 for approximately 10 days each month (totally 284 days or 51.120 net.hours). Fifteen mist nets were operated on ground level separately on three locations, which made 60 m line each. Mist nets were operated from 6.00 AM until 18.00 PM, and checked every two hours. The captured birds were identified, ringed, measured, weighted, photographed and released. In total 1061 individuals were captured during the study; those birds belong to 103 Species and 28 families. Based on guild, study area was dominated by insectivore-frugivore birds (IF) both in species and individual level. Meanwhile terrestrial frugivorous (TF) became the lowest one. Insectivore-frugivore also recorded has the broadest niche breadth. The bird communities dominated by small birds, which have weight lower than 30 g.

ABSTRAKMedia pembelajaran interaktif sangat diperlukan untuk membantu proses belajar mengajar, khususnya pada saat era pandemi COVID-19 yang lebih banyak menggunakan daring. Salah satu jenis media pembelajaran interaktif yang dapat digunakan secara efektif, efisien dan mudah yaitu aplikasi iSpring Suite 9 yang dapat digunakan terintegrasi dengan power point. Masih banyaknya guru yang belum mengetahui dan memahami penggunaan media pembelajaran interaktif dengan baik, sehingga tujuan dari kegiatan pengabdian masyarakat ini adalah melakukan sosialisasi kepada guru mengenai media pembelajaran iSpring Suite 9 di Pondok Pesantren Modern Darussalam Kabupaten Kepahiang, Provinsi Bengkulu. Kegiatan sosialisasi ini dilakukan dengan cara demo, yaitu pemberian penjelasan penggunaan iSpring serta dilanjutkan dengan praktek penggunaan media iSpring. Kegiatan sosialisai ini diikuti oleh guru dari berbagai bidang studi di Pondok Pesantren, sehingga ada banyak model atau beragam bentuk media yang dapat dihasilkan oleh guru. Pelaksanaan kegiatan berjalan dengan baik dan lancar serta sesuai dengan tahapan yang direncanakan. Adanya sosialisasi ini mampu membantu meningkatkan ketrampilan dan pengetahuan guru mengenai penggunaan salah satu aplikasi dalam penyusunan bahan ajar maupun dalam membuat media pembelajaran Kata kunci: iSpring, media pembelajaran interaktif, pondok pesantren

Jeruk Kalamansi (Citrofortunella microcarpa) merupakan komoditi khas Provinsi Bengkulu yang sekarang banyak dimanfaatkan untuk berbagai pengobatan, makanan dan kosmetika. Minyak atsiri dari limbah Kulit Jeruk Kalamanansi memiliki kandungan senyawa kimia diantaranya yaitu phenyl ethyl alcohol, geraniol, eugenol, dan beberapa senyawa lainnya yang berpotensi memiliki aktivitas antibakteri. Aktivitas antibakteri minyak atsiri kulit Jeruk Kalamansi dapat berpotensi sebagai antibakteri alami pada berbagai macam sediaan kosmetika salah satunya adalah deodorant spray. Tujuan penelitian ini adalah mendapatkan formula deodorant spray mengandung minyak atsiri kulit Jeruk Kalamansi yang memiliki aktivitas antibakteri terhadap bakteri penyebab bau badan yaitu Staphylococcus epidermidis. Dalam penelitian ini dilakukan formulasi deodorant spray dengan 3 formula F1, F2, F3 mengandung minyak atsiri 15%, 20% dan 25% serta F0 basis yang tidak mengandung minyak atsiri dan dibandingkan dengan F4 yaitu deodorant spray merek X yang beredar di pasaran. Pada setiap formula dilakukan uji aktivitas aktibakteri terhadap bakteri Staphylococcus epidermidis pada cawan petri menggunakan metode difusi cakram. Daerah diameter hambat pada masing-masing formula dihitung dan dibandingkan dengan menggunakan metode kualitatif dan kuantitatif. Hasil uji aktivitas antibakteri memperlihatkan bahwa F1 memiliki aktivitas antibakteri paling kuat.

The rhinoceros hornbill (Buceros rhinoceros) genetic characteristics consist of nucleotide polymorphisms, haplotypes, genetic distances, and relationships which are important for their conservation effort in Indonesia. We sequenced mitochondrial DNA D-loop hypervariable III fragments from five rhinoceros hornbill individuals at Safari Park Indonesia I and Ragunan Zoo, which were isolated using Dneasy® Blood and Tissue Kit Spin-Column Protocol, Qiagen. D-loop fragment replication was done by PCR technique using DLBuce_F (5'-TGGCCTTTCTCCAAGGTCTA-3') and DLBuce_R (5'-TGAAGG AGT TCATGGGCTTAG-3') primer. Thirty SNP sites were found in 788 bp D-loop sequences of five rhinoceros hornbill individuals and each individual had a different haplotype. The average genetic distance between individuals was 3.09% and all individuals were categorized into two groups (Group I: EC6TS, EC1RG, EC2TS and Group II: EC9TS, EC10TS) with a genetic distance of 3.99%. This result indicated that the two groups were distinct subspecies. The genetic distance between Indonesian and Thai rhinoceros hornbills was 10.76%. Five Indonesian rhinoceros hornbill individuals at Safari Park Indonesia I and Ragunan Zoo probably came from different populations, ancestors, and two different islands. This study can be of use for management consideration in captive breeding effort at both zoos. The D-loop sequence obtained is a useful character to distinguish three rhinoceros hornbill subspecies in Indonesia.