Eleutherine bulbosa (Mill.) Urb come from the Iridaceae family, this species contains secondary metabolites in the form of flavonoids and quinones. This research method includes Brine Shrimp Lethality Test (BSLT) toxicity using Artemia salina Leach larvae to determine the LC50 value. This study used experimental methods pharmacologically using a completely randomized design in the same direction pattern in the selection of test animals, 25 animals used were white mice divided into 5 groups with 5 mice per group, with 4 hours observed to find out toxic symptoms and continued observation every 24 hours to see death. Testing of BSLT results showed that ethanol extract had LC50 toxicity = 66.68 ppm (very toxic category), n-hexane fraction had LC50 toxicity = 47.64 ppm (very toxic category), chloroform fraction had LC50 toxicity = 295.1 ppm ( toxic category), and the water fraction has a toxicity of LC50 = 194.54 ppm (very toxic category). The chloroform fraction is an active fraction. Acute toxicity test LD50 value according to the calculation method of Miller Tainter Probit (187,499 mg/KgBB), the method of calculation of Thompson Weil (182,810 mg/KgBB), how to calculate Indonesian Pharmacopoeia (187,068 mg/KgBB). Based on the three calculations, the LD50 value is in the medium category (50-500 mg/kgBB).
Tujuan penelitian ini untuk mengetahui: 1) bagaiamana bentuk pengetahuan seksual anak remaja; 2) bagaimana dampak pengetahuan seksual terhadap perilaku seksual remaja. Jenis penelitian ini kualitatif deskriptif, informan dipilih melalui teknik purposive sampling dengan kriteria yaitu anak remaja Kecamatan Manggala Kota Makassar yang pernah mengikuti sosialisasi seks bebas, berusia 15-18 tahun. Teknik pengumpulan data yang digunakan yaitu wawancara, dan observasi. Data yang diperoleh dianalisis dengan tahapan mereduksi data, menyajikan data dan penarikan kesimpulan. Teknik pengabsahan data menggunakan triangulasi sumber. Hasil penelitian menunjukkan bahwa: 1) Bentuk pengetahuan seksual anak renaja di Kecamatan Manggala Kota Makassar, yaitu a) meliputi aspek pengetahuan siklus biologis (organ reproduksi), b) pengetahuan aspek norma dan batasan perilaku seksual, c) pengetahuan tentang peran dan fungsi secara seksual, dan d) Pengetahuan aspek kesehatan organ reproduksi. 2) Dampak pengetahuan seksual anak terhadap perilaku seks remaja di Kecamatan Manggala Kota Makassar, yaitu a) dampak positif: membantu menghindari tindakan penyimpangan dan abnormalitas seks, membantu mengidentifikasi baligh pada diri remaja dan memberikan pemahaman peran dari jenis jender; b) dampak negative: munculnya rasa penasaran yang tidak sehat dan munculnya perilaku menyimpang seksual akibat sosialisasi tidak sempurna.
Antioxidant and anticancer activity test on Leukemia cells L1210 were carried out on four fractions (ethanol extract, n-hexane, chloroform, and water fraction) of Eleutherine bulbosa [Mill.] Urb. The purpose of this research is to obtain IC50 antioxidant and anticancer activity of the active fraction of Eleutherine. bulbosa [Mill.] Urb on Leukemia cells L1210. The research method includes antioxidant activity test with DPPH (2,2-diphenyl-1-picrylhydrazyl) method, anticancer activity test of active fraction on Leukemia cell L1210, and GC-MS analysis to determine the active compound. Chloroform fraction has the best antioxidant activity with IC50 value 19.694 ppm (very strong category). The IC50 value of the chloroform fraction on L1210 Leukemia cells was 9.56 ppm (very strong category). Based on GC-MS analysis of the chloroform fraction, some compounds such as hexadecanoic acid, 9,12-octadecadienoic acid, linolenic acid, octadecanoic acid, androstan-17-one, and 1-(2,3,5,6-tetramethylphenyl)- ethanone were detected.
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