Current Czech schoolchildren showed a doubled prevalence of OW and OB during the last two decades and simultaneously during nearly three decades there were more than doubled prevalence of "poor" or "under normal" MF of children, with overall dramatic decrease of MF in current schoolchildren.

Objective: The marine n-3 fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) exert numerous beneficial effects on health, but their potency to defend against development of peripheral insulin resistance of healthy person with overweight remains poorly characterized. We aimed to evaluate the effect of a combination intervention using EPA + DHA and the lifestyle modification (LSM) in women with overweight. Method: In a parallel-group, three-arm, randomized trial (UMIN Clinical Trials Registry-R000031131), 34 women were assigned to a 12-weekintervention using corn oil (1.5 g/day; placebo); LSM and corn oil (1.5 g/day; LSM); or LSM and EPA + DHA concentrate (1.5 g/day, containing ~ 0.6 g EPA + DHA; LSM & n-3). At baseline and after intervention, anthropometric measurements including bioelectrical impedance analysis, spiroergometry, 24-hours dietary recall, and various metabolic markers, adiponectin and cytokines were evaluated in serum using standard procedures. Data from 29 women were used for the final evaluation. Wilcoxon two-sided rank-sum test was used to inspect the differences between LSM and LSM & n-3, and placebo groups, with a p-value of ≤ 0.05. All computations were performed with MATLAB Statistics Toolbox. Results: In comparison with placebo, LSM and LSM & n-3 decreased body weight, waist circumference, and body fat, and increased VO 2 max/kg. LSM & n-3 increased adiponectin levels in comparison to LSM. Fasting insulin, IL8, and cholesterol were decreased by LSM, but were unchanged by LSM & n-3. IL6 was not affected in LSM & n-3, while it was increased in LSM. Other inflammatory markers, as well as leptin, LIF, follistatin, BDNF, and fasting triacylglycerol were not significantly affected by any of the interventions. Conclusion: Besides preventing a modest negative effect of LSM on IL6 and adiponectin level, the combination of LSM and EPA + DHA supplementation could be probably used to improve the functional capacity of adipose tissue in women with overweight.

Emerging evidence indicates that polychlorinated biphenyls (PCBs) are involved in the development of diabetes mellitus in the obese. The purpose of this study was to determine mechanisms by which PCB 153 (2,2′,4,4′,5,5′-hexachloro-biphenyl) could influence diet-induced obesity and insulin resistance during adipogenesis. Lineage of h-ADMSCs was differentiated either as control (differentiation medium only), or with lipid vehicle modeling high fat nutrition (NuTRIflex) or lipid free vehicle (dimethylsulfoxide) for 28 days with or without PCB 153 daily co-exposure (in three concentrations 0.1, 1, and 10 µM). Gene expression analyses were performed using RT-qPCR at days 4, 10, 21, 24, 28; protein levels Akt and phosphorylated Akt (Phospho-Akt) by Western blot at days 4, and 21. PCB 153 treatment of h-ADMSCs only in lipid vehicle was associated with down regulation of key master genes of adipogenesis: PPARγ, SREBP-1, PPARGC1B, and PLIN2 during the whole process of differentiation; and with increased Akt and decreased Phospho-Akt protein level at day 21. We have shown that PCB 153, in concentration 0.1 µM, has a potential in lipid rich environment to modulate differentiation of adipocytes. Because European and U.S. adults have been exposed to PCB 153, this particular nutrient-toxicant interaction potentially impacts human obesity and insulin sensitivity.

Nowadays, a great attention is paid to the biological activity of plant polyphenols and their potential importance for the human health. Therefore knowledge regarding the dietary intake of polyphenols and their particular subclasses has gained interest. In this report, the results of a pilot study evaluating the average polyphenol content in the Czech diet have been presented.Knowledge of the average intake of plant polyphenols is an important contribution to the evaluation of the dietary pattern from the aspect of its health impact.An annual average consumption of the main foods of plant origin (a total of 80 commodities) was estimated, using data from the Czech annual statistical report, in the entire Czech population in 2013. These values (kg/y) were multiplied with the contents of plant polyphenols in the same items as presented in the in the database Phenol Explorer.The average intake of plant polyphenols was 426 mg/d. The prevailing polyphenols were chlorogenic acid, 82 mg/d (most important sources were potatoes, coffee, plums), followed by apigeninfl avone, 79 mg/d (wheat), heneicosylresorcinol, 38 mg/d (wheat), ferrulic acid, 17 mg/d (wheat) and anthocyanin malvidin, 13 mg/d (red wine). These values are below the intake of polyphenols in the most EU countries.These differences refl ect -inter alia -the fact that beer having low content of polyphenols is a dominant commodity in Czech dietary pattern while fruit and vegetables as well as teas and coffee consumption is relatively low.

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