Sucrose content in soybean seeds is desired to be high because as a sweetness-imparting component, it helps in wider acceptance of soy-derived food products. Conversely, galactosyl derivatives of sucrose, that is, raffinose and stachyose, which are flatulence-inducing components, need to be in low concentration in soybean seeds not only for augmenting utilization of the crop in food uses but also for delivering soy meal with improved metabolizable energy for monogastric animals. In the present study, analysis of 148 soybean genotypes for sucrose and total raffinose family oligosaccharides (RFOs) contents revealed a higher variation (4.80-fold) for sucrose than for RFOs content (2.63-fold). High-performance liquid chromatography analyses revealed ranges of 0.64-2.53 and 2.09-7.1 mmol/100 g for raffinose and stachyose contents, respectively. As information concerning the environmental effects on the sucrose and RFOs content in soybean seeds is not available, we also investigated a set of seven genotypes raised at widely different geographical locations for these quality traits. Sucrose content was found to be significantly higher at cooler location (Palampur); however, differences observed for raffinose and stachyose contents across the growing locations were genotype-dependent. The results suggest that soybean genotypes grown at cooler locations may be better suited for processing soy food products with improved taste and flavor.

Aims:To Study on the Effect of nitrogen levels on growth and yield of mustard (Brassica juncea Curzen and Cross.) varieties under late sown condition Study Design: The Field experiment was laid out in a Factorial randomized block design used in this study.

We evaluated the analytical performance of the "SimulTROPIN" (Becton-Dickinson Inc.) radioimmunoassay for the simultaneous measurement of lutropin (LH) and follitropin (FSH) in human serum. Dose response, linearity, analytical recovery, sensitivity, and reagent stability were all acceptable. Cross reactivity with other structurally related hormones [choriogonadotropin (CG) and thyrotropin (TSH)] was minimal. A normal reference interval was established for nonmidcycle, ovulatory women. We confirm the low degree of CG and TSH cross reactivity with LH and FSH reported by Becton Dickinson and conclude that this assay is an acceptable method for laboratory use in the simultaneous quantification of these analytes.

No abstract

No abstract

No abstract

We evaluated the analytical and clinical performance of three "sensitive" immunoradiometric assay (IRMA) kits (Tandem-R TSH HS, Hybritech, Inc.; EchoClonal TSH, Bio-Rad; Coat-A-Count TSH IRMA, Diagnostic Products Corp.) for measurement of thyrotropin (TSH) and compared their performance against a "regular" IRMA (ARIA-HT, Becton-Dickinson) to determine whether these assays might eliminate the need to perform the thyroliberin (TRF) stimulation test. We concluded that the Tandem and EchoClonal kits may obviate the need to perform TRF stimulation tests in some patients. Using only the basal TSH concentration to predict the TSH response to TRF, we found all three sensitive TSH assays to be useful for detecting abnormal thyrotroph function. Dose-response, linearity, analytical recovery, and specificity were acceptable for all kits, but intra-assay precision at very low TSH concentrations and analytical sensitivity differed considerably among the kits. Using the EchoClonal assay, we established a normal reference interval for TSH of 0.4-4.6 milli-int. units/L.

No abstract