Evaluation of simultaneous measurement of lutropin and follitropin with the SimulTROPIN radioimmunoassay kit.

Wians, Frank H.; Dev, Jai; Powell, Mackenzie; Heald, J I

doi:10.1093/clinchem/32.5.887

Cited by 31 publications

(8 citation statements)

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“…Various multi-analyte methods using enzymes, radiolabels, uorescent or quantum dots as labels and a number of nonlabeled methods have been developed in molecular biology, food safety, and clinical research. [4][5][6][7][8] Despite progress, none of these assays has achieved widespread use, probably because they are technically complex, costly, labor-intensive and timeconsuming. Thus, exploring new strategies for sensitive and convenient multi-analyte detection with high throughput in clinical diagnosis is of great interest.…”

Section: Introductionmentioning

confidence: 99%

Magnetic particle-based time-resolved fluoroimmunoassay for the simultaneous determination of α-fetoprotein and the free β-subunit of human chorionic gonadotropin

Hou

Liu

Ren

et al. 2013

Analyst

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In this paper, a novel time-resolved fluoroimmunoassay (TRFIA) protocol using magnetic particles for the simultaneous determination of α-fetoprotein (AFP) and the free β-subunit of human chorionic gonadotropin (free β-hCG) in human serum is described. The new approach uses magnetic particles as an immobilization matrix and means of separation, while the luminescent europium and samarium chelates are used as probes. The proposed method was evaluated via a single-step, sandwich-type TRFIA immunoassay of AFP and free β-hCG as model analytes in serum. With the advantages of magnetic particles, the TRFIA immunoassay exhibited a wide dynamic range for AFP of 0.1-750 ng mL(-1), with a lower detection limit of 0.05 ng mL(-1). The dynamic range for free β-hCG was 0.16-450 ng mL(-1), with a lower detection limit of 0.08 ng mL(-1). Satisfactory specificity, reproducibility, and recovery of the immunoassay were demonstrated. Good correlations were obtained in the analysis of 446 human serum samples between the proposed method and a commercial TRFIA kit. These results demonstrate the feasibility and potential of the new method as a rapid and highly sensitive immunoassay that could be developed into a platform for multi-analyte determinations in clinical practice.

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Section: Introductionmentioning

confidence: 99%

Magnetic particle-based time-resolved fluoroimmunoassay for the simultaneous determination of α-fetoprotein and the free β-subunit of human chorionic gonadotropin

Hou

Liu

Ren

et al. 2013

Analyst

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“…One approach is to use dual labels for two analyte analysis, thereby performing two independent assays on the same solid phase. It can be based on the use of two different enzymes [1], radioactive markers [2], fluorophores [3,4], metal-labels [5] or others. If only a single label is used but no cross-reactivity occurs, spatial resolution allows more complex multianalyte analysis as was described for the multispot immunoassay based on fluorescence detection {6~8J.…”

Section: Introductionmentioning

confidence: 99%

An immunoarray for the simultaneous determination of multiple triazine herbicides

Wortberg

Kreissig

Jones

et al. 1995

Analytica Chimica Acta

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An immunochemical method for simultaneous analysis of cross-reacting analytes is presented. We demonstrate the general principle using triazine herbicides as the model system. The analysis is based on a combination of individual enzyme immunoassays (immunoarray) for triazine herbicides using antibodies with different cross-reactivity patterns towards the selected analytes. The assay signals obtained can be mathematically evaluated to estimate concentrations of each analyte out of a ternary or quaternary mixture. The mathematical model utilizes an extension of the empirical four parameter log-logistic fit. Using mono-and polyclonal antibodies it was possible to quantify the four analytes atrazine, simazine, cyanazine, and prometon in the low to sub-ppb range simultaneously.

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“…Orangeburg, NY) was used for method comparison. The Simultrac assay is identical to the Becton-Dickinson Simultropin assay described by Beinlich et al (1) and Wians et al (2), except that the Simultropin kits uses FSH standards calibrated against the WHO 2nd International Reference Preparation (IRP) 7 1 /333, whereas the Simultrac kit calibrates against the WHO 2nd IRP 781549; both assays use LH standards calibrated against the WHO 1st IRP 68/40.…”

Section: Introductionmentioning

confidence: 99%

Assay of follitropin and lutropin by fluorescence enzyme immunoassay

Armbruster¹,

Armbruster²,

Hawes³

1990

Clinical Laboratory Analysis

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Recently developed fluorescent enzyme immunoassays (FEIs) for follitropin (FSH) and lutropin (LH) designed for the Stratus analyzer were compared with a typical dual FSH-LH radioimmunoassay (RIA) procedure. The FEIs use monoclonal antibodies in a sandwich reaction scheme similar to that of immunoradiometric procedures but with filter paper tabs rather than polystyrene beads and alkaline phosphatase as a tag instead of a radioisotope. Precision of the FEI was comparable with that of the RIA tests with CVs typically under 10%. The detection limit of the FSH test is 0.4 IU/L and that of the LH test is 0.6 IU/L. Measurable carryover exists but is clinically inconsequential. Neither assay exhibits total specificity, but the degree of cross-reactivity is unlikely to be problematic in most situations. Recovery with the FSH assay was 103%, with the LH assay, it averaged 108%. Moderate amounts of bilirubin and triglycerides and gross hemolysis caused no significant interferences. Method comparison studies yielded the following: FEI for FSH, 1.16 RIA + 3.1, r = 0.97, n = 114; FEI for LH, 0.77 RIA - 0.23, r = 0.96, n = 112. Difference analysis of the method comparison data revealed relatively poor agreement between the FEI tests and the dual RIA procedure. With the adoption of method-specific normal reference ranges, the FEI tests offer attractive alternatives to radioisotopic FSH and LH assays.

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Evaluation of simultaneous measurement of lutropin and follitropin with the SimulTROPIN radioimmunoassay kit.

Cited by 31 publications

References 0 publications

Magnetic particle-based time-resolved fluoroimmunoassay for the simultaneous determination of α-fetoprotein and the free β-subunit of human chorionic gonadotropin

Magnetic particle-based time-resolved fluoroimmunoassay for the simultaneous determination of α-fetoprotein and the free β-subunit of human chorionic gonadotropin

An immunoarray for the simultaneous determination of multiple triazine herbicides

Assay of follitropin and lutropin by fluorescence enzyme immunoassay

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