Sucrose and Raffinose Family Oligosaccharides (RFOs) in Soybean Seeds As Influenced by Genotype and Growing Location

Kumar, Vineet; Rani, Anita; Goyal, Lokesh; Dixit, Amit Kumar; Manjaya, J. G.; Dev, Jai; Swamy, M.S.

doi:10.1021/jf903141s

Cited by 112 publications

(121 citation statements)

References 14 publications

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“…In our experiment, WLM contained comparatively high levels of RSO (8.26 ± 0.14 g/100 g). High amounts of RSOs were also found in SBM, but these levels were lower than those in lupin, which corroborates the findings of other authors (Kumar et al 2010;Svejstil et al 2015). According to Zdunczyk et al (2014), the inclusion of blue lupin seeds (20%) to a layer diet can increase the RSO content (from 0.77 to 2.08% DM).…”

Section: Discussionsupporting

confidence: 89%

Effect of dietary lupin (Lupinus albus) on the gastrointestinal microbiota composition in broiler chickens and ducks

Geigerová¹,

Švejstil²,

Skřivanová³

et al. 2017

CZECH J ANIM SCI

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Geigerová M., Švejstil R., Skřivanová E., Straková E., Suchý P. (2017): Effect of dietary lupin (Lupinus albus) on the gastrointestinal microbiota composition in broiler chickens and ducks. Czech J. Anim. Sci., 62, 369-376.The purpose of the study was to evaluate the amount of raffinose-series oligosaccharides (RSO) in soybean meal (SBM), whole white lupin seed meal (WLM), sunflower meal (SFM), and rapeseed oil meal (ROM) and to determine whether partial or complete dietary WLM replacement affected the numbers of bacteria in selected groups in the microbiota of broiler chickens and ducks without inducing any weight loss. Total counts of anaerobes, lactobacilli, bifidobacteria, and Escherichia coli in caecal samples from both ducks and broiler chickens, as well as in a crop chyme, in broiler chickens, were determined. Live weights before slaughter were determined. Both broiler chickens and ducks were fed a control diet with SBM (L 0 ) or diet containing 50% or 100% WLM as a substitute for SBM (groups L 50 and L 100 , respectively). In comparison with SBM, WLM contained significantly higher amounts of RSO, and the amounts of oligosaccharides in SFM (1.73 ± 0.26 g/100 g) and ROM (1.79 ± 0.14 g/100 g) were negligible compared to those in WLM (8.26 ± 0.14 g/100 g) and SBM (6.96 ± 0.21 g/100 g). The inclusion of lupin in chicken diets did not significantly affect the monitored bacterial groups in crop chyme, but a complete replacement of SBM with WLM (L 100 group) in chicken diets significantly (P ≤ 0.05) increased the counts of lactobacilli in caecal samples. Partial (L 50 group) and complete (L 100 group) lupin supplementation in the duck diet significantly (P ≤ 0.05) increased counts of lactobacilli and bifidobacteria by at least one order of magnitude. E. coli counts in poultry were not affected by changes in diet. The results of our study indicate that partial dietary replacement of SBM with WLM did not significantly affect the live weight of broiler chickens and ducks, but that complete replacement of SBM with WLM may lead to weight loss.

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Section: Discussionsupporting

confidence: 89%

Effect of dietary lupin (Lupinus albus) on the gastrointestinal microbiota composition in broiler chickens and ducks

Geigerová¹,

Švejstil²,

Skřivanová³

et al. 2017

CZECH J ANIM SCI

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“…The specific availability of raffinose in mucosal secretions has not been determined. Raffinose and stachyose are common galactooligosaccharides, found in large amounts in plants and plant products used as staple human foods, such as beans and soy (11,26). They are not fermented by our bodies but are thought to be substrates for the intestinal microflora (50).…”

Section: Discussionmentioning

confidence: 99%

Role of Dihydrolipoamide Dehydrogenase in Regulation of Raffinose Transport in Streptococcus pneumoniae

Tyx

Håkansson

2011

J Bacteriol

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Streptococcus pneumoniae strains lacking the enzyme dihydrolipoamide dehydrogenase (DLDH) show markedly reduced ability to grow on raffinose and stachyose as sole carbon sources. Import of these sugars occurs through the previously characterized raffinose ATP-binding cassette (ABC) transport system, encoded by the raf operon, that lacks the necessary ATP-binding protein. In this study, we identified the raffinose ATP-binding protein RafK and showed that it was directly involved in raffinose and stachyose import. RafK carries a C-terminal regulatory domain present in a subset of ATP-binding proteins that has been involved in both direct regulation of transporter activity (inducer exclusion) and transcription of transporter genes. Pneumococci lacking RafK showed a 50-to 80-fold reduction in expression of the raf operon genes aga (alpha-galactosidase) and rafEFG (raffinose substrate binding and permease genes), and both glucose and sucrose inhibited raffinose uptake through inducer exclusion. Like RafK, the presence of DLDH also activated the expression of raf operon genes, as DLDH-negative pneumococci showed a significantly decreased expression of aga and rafEFG, but DLDH did not regulate rafK or the putative regulatory genes rafR and rafS. DLDH also bound directly to RafK both in vitro and in vivo, indicating the possibility that DLDH regulates raffinose transport by a direct interaction with the regulatory domain of the transporter. Finally, although not as attenuated as DLDHnegative bacteria, pneumococci lacking RafK were significantly outcompeted by wild-type bacteria in colonization experiments of murine lung and nasopharynx, indicating a role for raffinose and stachyose transport in vivo.Dihydrolipoamide dehydrogenases (DLDH) are enzymes classically involved in the reoxidation of dihydrolipoamide during conversion of 2-oxo acids (such as pyruvate) in several multienzyme complexes in the central metabolism (34, 52). However, previous work from our and another laboratory have shown that DLDH is not involved in metabolizing 2-oxo acids in the pneumococcus, indicating that DLDH must serve other functions (41,42). This is supported by evidence that organisms that lack 2-oxo acid dehydrogenases still express a DLDH protein (9, 10). One such function was suggested by Richarme in the 1980s when he showed that Escherichia coli strains that fail to make lipoic acid or strains treated with an inhibitor that mainly inhibits DLDH function resulted in reduced import of galactose, maltose, and ribose through ATP-binding cassette (ABC) transporters (35). We then showed that inactivation of DLDH in the pneumococcus results in an inability of the bacteria to import and utilize galactose and the alpha-galactoside sugars raffinose and stachyose and that a lack of DLDH is associated with an almost complete attenuation of this strain in animal infection experiments (41).Transport of carbohydrates and other energy sources is important for many aspects of bacterial life and therefore highly regulated. Fitness in the bacterial host ...

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“…Similarly, KarrLilienthal et al (2004) reported that pigs fed diets containing SBM from India had lower CP digestibility. It has been reported that different growing conditions and the use of various soybean genotypes in different regions can affect nutrient composition, including contents of oligosaccharides, protease inhibitors and fiber (Kumar et al, 2010;Baker and Stein, 2009). As a result, nutrient digestibility in different batches of SBM fed to pigs may differ (Karr-Lilienthal et al, 2005).…”

Section: Nutrient Digestibilitymentioning

confidence: 99%

Effects of Origins of Soybean Meal on Growth Performance, Nutrient Digestibility and Fecal Microflora of Growing Pigs

Yun¹,

Ingale²,

Lee³

et al. 2013

Journal of Animal Science and Technology

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This experiment was conducted to investigate the effects of soybean meal (SBM) of different origin (Korea, India or Brazil) on performance, fecal microflora and economics of grower pigs. A total of 144 grower pigs (initial BW, 10.4 kg) were randomly allotted to six treatments on the basis of BW. Pig's diets containing SBM were formulated based on the total amino acid (TAA) or true ileal digestible amino acid (TIDAA) levels. Pigs fed domestic SBM diets had greater (p < 0.05) overall ADG, ADFI and final body weight and apparent total tract digestibility (ATTD) of DM, GE and CP (phases I, II and III) than those fed diets containing SBM originated from India or Brazil. Moreover, greater (p < 0.05) ADG, ADFI, feed /gain (F/G), and ATTD of DM, GE and CP were observed in response to diets formulated on a TIDDA basis when compared with those formulated on a TAA basis. The fecal microflora was not affected (p > 0.05) by dietary treatments. The overall feed cost per kg body weight gain was less (p < 0.05) for diets formulated using domestic SBM than for those containing SBM from India, while the feed cost per kg body weight gain was less (p < 0.05) for diets formulated prepared on a TIDAA than a TAA basis. These results indicate that domestic SBM has better quality than SBM imported from Brazil or India and better performance was obtained when diets were formulated based on the true ileal digestible amino acid (TIDAA) content.

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Sucrose and Raffinose Family Oligosaccharides (RFOs) in Soybean Seeds As Influenced by Genotype and Growing Location

Cited by 112 publications

References 14 publications

Effect of dietary lupin (Lupinus albus) on the gastrointestinal microbiota composition in broiler chickens and ducks

Effect of dietary lupin (Lupinus albus) on the gastrointestinal microbiota composition in broiler chickens and ducks

Role of Dihydrolipoamide Dehydrogenase in Regulation of Raffinose Transport in Streptococcus pneumoniae

Effects of Origins of Soybean Meal on Growth Performance, Nutrient Digestibility and Fecal Microflora of Growing Pigs

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