Effect of dietary lupin (Lupinus albus) on the gastrointestinal microbiota composition in broiler chickens and ducks

Abstract

Geigerová M., Švejstil R., Skřivanová E., Straková E., Suchý P. (2017): Effect of dietary lupin (Lupinus albus) on the gastrointestinal microbiota composition in broiler chickens and ducks. Czech J. Anim. Sci., 62, 369-376.The purpose of the study was to evaluate the amount of raffinose-series oligosaccharides (RSO) in soybean meal (SBM), whole white lupin seed meal (WLM), sunflower meal (SFM), and rapeseed oil meal (ROM) and to determine whether partial or complete dietary WLM replacement affected the numbers of bacteria in selected groups in the microbiota of broiler chickens and ducks without inducing any weight loss. Total counts of anaerobes, lactobacilli, bifidobacteria, and Escherichia coli in caecal samples from both ducks and broiler chickens, as well as in a crop chyme, in broiler chickens, were determined. Live weights before slaughter were determined. Both broiler chickens and ducks were fed a control diet with SBM (L 0 ) or diet containing 50% or 100% WLM as a substitute for SBM (groups L 50 and L 100 , respectively). In comparison with SBM, WLM contained significantly higher amounts of RSO, and the amounts of oligosaccharides in SFM (1.73 ± 0.26 g/100 g) and ROM (1.79 ± 0.14 g/100 g) were negligible compared to those in WLM (8.26 ± 0.14 g/100 g) and SBM (6.96 ± 0.21 g/100 g). The inclusion of lupin in chicken diets did not significantly affect the monitored bacterial groups in crop chyme, but a complete replacement of SBM with WLM (L 100 group) in chicken diets significantly (P ≤ 0.05) increased the counts of lactobacilli in caecal samples. Partial (L 50 group) and complete (L 100 group) lupin supplementation in the duck diet significantly (P ≤ 0.05) increased counts of lactobacilli and bifidobacteria by at least one order of magnitude. E. coli counts in poultry were not affected by changes in diet. The results of our study indicate that partial dietary replacement of SBM with WLM did not significantly affect the live weight of broiler chickens and ducks, but that complete replacement of SBM with WLM may lead to weight loss.

Selected blood biochemical indicators of Cherry Valley ducks undergoing fattening in relation to their diet and sex

“…Dietary use of lupine seeds is currently studied for its role in human as well as animal nutrition. Due to its high amount of protein, ranging from 29 to 41% (Strakova et al 2006;Saastamoinen et al 2013), which is comparable to the level of protein in soy beans, sweet lupine varieties with low alkaloid levels are currently included as a vegetable protein source in human nutrition as well as in feeds for animals (Zraly et al 2008;Volek et al 2014;Geigerova et al 2017;Prusinski 2017). White lupine varieties are easier to grow under the soil and climatic conditions of the Czech Republic than yellow lupine varieties.…”

“…Pilvi et al (2006) reported a positive effect of lupine proteins on blood pressure and vessel endothelium function in rats with hypertension. Except for the protein content itself, especially the presence of arginine, which is often deficient in poultry feeds (Suchy et al 2006), seeds of certain varieties of white lupine are appreciated also as a considerable source of n-3 PUFA (Zapletal et al 2015) and also as a major source of oligosaccharides of the raffinose series, which exert positive prebiotic effects on poultry health (Geigerova et al 2017).…”

Beneficial effects of substituting soybean meal for white lupin (Lupinus albus, cv. Zulika) meal on the biochemical blood parameters of laying hens

“…Krawczyk et al (2015) as well as Rutkowski et al (2017) reported that it is possible to incorporate lupin into the diet for laying hens as a partial replacement for soya meal without any negative effect on the yield of laying hens or the physico-chemical or sensory properties of the eggs. Experiments using diets with lupin protein were also done in fattening chickens (Suchy et al 2010;Geigerova et al 2017;Al-Sagan et al 2020), where the influence of lupin protein on production parameters was observed. These, above mentioned authors who were focussing on the production parameters of poultry, found mostly benefits for substitution of soya meal for lupin meal.…”

An in Vivo Study for the Use of Lupinus Albus (Fabaceae) in Drosophila Melanogaster Diet

“…It is essential to know that the organism is not damaged when using substitute products in nutrition. For example, the addition of Lupin to the chicken diets for five weeks (20%) did not change the growth performance 15 ; it was digested by sheep 16 , it can be used up to 30% in rainbow trout diet 17 ; it was used for feeding chicken and ducks; it did not significantly affect 18 the blood parameters (50 and 100%); it did not cause a significant change in monocytes 19 ; the addition of up to 20% of the diet to the chicken health did not have any negative effects 20 . In human studies, it is known that Lupin meals taken daily with 12 mg (0.02%) oral diet as a food are digestible and did not cause a significant change in blood parameters 11 .…”