Sum~nary. In search for new techniques of renal preservation, kidneys were persuf~ated in situ with gaseous 02 at a pressure of 90--100 mm Hg via their vascular system (orthograde aerobic ischemia). The results on post-isehemie function and blood flow, and on the changes in the renal tissue levels of the adenine nucleotides were compared to those obtained from kidneys ischemically stored without additional measures (anaerobic ischemia). The kidneys were stored for 4 hrs at 6°C. A contralateral nephrectomy was performed before the preservation period.All dogs subjected to renal anaerobic ischemia survived. After orthograde aerobic ischemia, 3 out of 4 dogs died from uremia within 8 days, 1 animal survived. Although the aerobic isehemic preservation resulted in the maintenance of a metabolic status characteristic for aerobiosis, post-ischemic renal function recovered too slowly and insufficiently on the average. Renal blood flow was within normal ranges after anaerobic ischemia, it was reduced to about one third of this after orthograde aerobic isehemia.
with the cooperation of C. Freiberg, D. A m m e r m a n n , and M. BrunkePfliigers Archiv 337, 87--106 (1972) Page 88, second line from the top. The sentence reads as follow: It must be attributed to a sufficient aerobiosis in the aerobically ischemic kidney, during which organogenic substrates and substrates brought in by the initial perfusion are utilized.
Summary. I~etrograde persufflation of isehemie kidneys of dogs via the renal vein with gaseous 02 was evaluated as a method of kidney preservation (retrograde aerobic isehemia) and compared to conventional ischemic storage without additional measures (anaerobic isehemia). Renal preservation in situ lasted 4 hrs at 6°C. A eontralateral nephreetomy was performed before the preservation period. For retrograde aerobic isehemia the persufflation pressure was 30 mm Hg, the gas flowed off via opened capsular vessels.While dogs which had undergone renal anaerobic ischemia showed a marked temporary post-ischemic increase in renal blood flow and in the systemic blood pressure, dogs submitted to renal retrograde aerobic ischemia did not exhibit a storage induced loss of the renal vascular autoregulation. During and after retrograde aerobic isehemia the renal tissue levels of ATP, ADP, and AMP remained within normal ranges; ATP and the sum of adenine nucleotides decreased markedly during anaerobic isehemia. In both the groups a normal renal function was resumed again; but the recovery time of various parameters of renal function (clearance from inulin and PAH, urea excretion, serum concentrations of urea and creatinine) was shorter after retrograde aerobic isehemia than after anaerobic isehemia. Kidneys subjected to retrograde aerobic ischemia exhibited a better functional adaption to the contralateral nephreetomy during the 3 weeks of post-ischemic observation than kidneys stored in anaerobic isehemia.
Key words: Renal preservation --Anaerobic ischemia --Aerobic ischemia --Retrograde renal 03 persufflation --Post-ischemic renal function.In search for new approaches to renal preservation, ischemie kidneys were persuffluted with humidified gaseous oxygen. According to biochemical parameters an O3 persufflation via the arteria or vena renalis * Supported by a grant from the Deutsche Forschungsgemeinschaft in the ,,Sonderforschungsbereieh 68". 3*
The capacity for recovery of the normothermic left ventricular myocardium from a regional complete ischemia (RCI) was investigated using changes in the myocardial metabolic status (ATP, ADP, AMP, creatine phosphate (CrP), free creatine, glycogen, glucose, lactate) and alterations of the morphology as parameters. In dogs, an area of the anterior wall of the left ventricular myocardium was temporarily deprived completely of its blood supply by 5–7 overlapping ligatures extending into the heart cavity. The metabolites of the adenylic acid-CrP system returned to normal tissue levels after 30 and 60 min of RCI within 14 and 35 days of recovery, respectively; restoration averaged 82% after 100 min, 74% after 140 min, and 38% after 180 min of RCI after 5 weeks of recovery. At the same time glycogen amounted to 163% after 100 min, 114% after 140 min, and 65% after 180 min of RCI. The biochemical data correlated well with the structural changes in the affected myocardium, especially with the amount of de- and regenerating heart muscle cells. These obviously were functionally defect and were not comparable with normal structured and functioning heart muscle cells.
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