Metabolic and Structural Recovery of Left Ventricular Canine Myocardium from Regional Complete Ischemia

Abstract

The capacity for recovery of the normothermic left ventricular myocardium from a regional complete ischemia (RCI) was investigated using changes in the myocardial metabolic status (ATP, ADP, AMP, creatine phosphate (CrP), free creatine, glycogen, glucose, lactate) and alterations of the morphology as parameters. In dogs, an area of the anterior wall of the left ventricular myocardium was temporarily deprived completely of its blood supply by 5–7 overlapping ligatures extending into the heart cavity. The metabolites of the adenylic acid-CrP system returned to normal tissue levels after 30 and 60 min of RCI within 14 and 35 days of recovery, respectively; restoration averaged 82% after 100 min, 74% after 140 min, and 38% after 180 min of RCI after 5 weeks of recovery. At the same time glycogen amounted to 163% after 100 min, 114% after 140 min, and 65% after 180 min of RCI. The biochemical data correlated well with the structural changes in the affected myocardium, especially with the amount of de- and regenerating heart muscle cells. These obviously were functionally defect and were not comparable with normal structured and functioning heart muscle cells.

Adenine nucleotide content and regional function during ischemia and reperfusion in canine ventricular myocardium

“…These observations are consistent with results obtained with different models by several investigators (Isselhard et al, 1975;Schaper et al, 1979). These observations are consistent with results obtained with different models by several investigators (Isselhard et al, 1975;Schaper et al, 1979).…”

“…These observations are consistent with results obtained with different models by several investigators (Isselhard et al, 1975;Schaper et al, 1979). The overshoot of CP level observed by us and others (Isselhard et al, 1975;Vial et al, 1978;Schaper et al, 1979) may reflect a disturbance in the mass action ratio of creatine kinase or the reduction of the mechanical work in the post-ischemic myocardium. Furthermore the rapid synthesis of CP observed after reperfusion despite the low cellular level of ATP, proves, that mitochondrial energy production by oxidative phosphorylation was still effective.…”

“…The most prominent and prolonged metabolic effect of transient myocardial ischemia is the significant depletion of ATP and total adenine nucleotides (Isselhard et al, 1975;Vial et al, 1978;Schaper et al, 1979). This loss of intracellular adenine nucleotides (AdN) is known to be due to the formation of adenosine from AMP by 5'-nucleotidase and to the subsequent diffusion of adenosine out of the myocardial cells (Rubio et al, 1973).…”

Επίδραση Αλοθανίου Και Ισοφλουρανίου Στο Μεταβολισμό Του Μυοκαρδίου Σε Ασθενείς Με Στεφανιαία Νόσο

“…Η αντίδραση αυτή απαιτεί την 41 . Έτσι, στον σκύλο παρατηρείται ότι μετά από απόφραξη του προσθίου κατιόντος για 30 ή 60 λεπτά, η επάνοδος των τιμών του ΑΤΡ και των αδενινικών νουκλεοτιδίων στις φυσιολογικές τιμές απαιτεί 14 ημέρες 87 .…”