Metabolism of canine kidneys in anaerobic ischemia and in aerobic ischemia by persufflation with gaseous oxygen

Isselhard, W.; Berger, M.; Denecke, H. J.; Witte, J; Fischer, J.; Molzberger, H; Freiberg, Christoph; Ammermann, D.; Brunke, M.

doi:10.1007/bf00587393

Cited by 20 publications

(12 citation statements)

References 24 publications

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“…0.001 atm). After tissue water was evaporated during at least 5 days of freeze-drying, the tissue concentrations of energy-rich adenine nucleotides and lactate were determined by standard enzymatic tests, as described previously [10,11]. The energy charge potential (ECP) was calculated according to Atkinson [12] as: ECP = (ATP + ½ ADP)/(ATP + ADP + AMP) (total adenine nucleotides, TAN = ATP + ADP + AMP).…”

Section: Metabolic Analysismentioning

confidence: 99%

Experimental Liver Preservation with Celsior:A Novel Alternative to University of Wisconsin and Histidine-Tryptophan-α-Ketoglutarate Solutions?

Tolba

Akbar²,

Müller³

et al. 2000

Eur Surg Res

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Celsior, a low viscosity and low potassium preservation solution, has recently been tested successfully in the cold preservation of heart, lung, kidney and small intestine. The purpose of the present study was to evaluate the potential of Celsior in the cold preservation of the liver. Livers were harvested from male Wistar rats and then flushed with either Celsior (CE), University of Wisconsin solution (UW) or histidine-tryptophan-α-ketoglutarate solution (HTK) and stored for 24 h at 4°C in the respective solution. The reperfusion was performed in vitro using a recirculating model with oxygenated (95% O₂, 5% CO₂) Krebs-Henseleit buffer at 37°C. To simulate the slow rewarming during the surgical implantation in vivo, all livers were stored for 30 min at room temperature prior to reperfusion. After ischemic storage and also after reperfusion some samples were freeze-clamped for analysis of tissue metabolites while others were tested for structural and functional integrity by the isolated perfusion. CE vs. UW vs. HTK: Metabolic preservation of tissue ATP (μmol/g dry weight) during cold storage was best with Celsior (0.46 ± 0.17 vs. 0.26 ± 0.03 vs. 0.35 ± 0.07; p < 0.05 CE vs. UW), but upon reperfusion energetic recovery was comparable in the three groups (3.45 ± 0.66 vs. 4.27 ± 0.41 vs. 3.63 ± 0.64 μmol/g/dry weight). There appeared to be structural integrity during reoxygenation irrespective of the used preservation solution with comparable values of parenchymal enzyme release (ALT: 575 ± 82 vs. 547 ± 106 vs. 593 ± 38 mU/g/l), bile production (18.0 ± 1.0 vs. 18.5 ± 2.5 vs. 18.7 ± 1.4 μl/g/ min), and the release of acid phosphatase, an indicator for activated Kupffer cells (89 ± 13 vs. 90 ± 5 vs. 123 ± 21 mU/g/l) in this in vitro model. Vascular flow characteristics were approximated by the portal perfusion pressure, which tended to be elevated upon initial reperfusion in the UW group (8.4 ± 0.6 mm Hg) compared to 6.6 ± 1.0 and 7.3 ± 0.4 mm Hg in Celsior and HTK, respectively. However, the pressure values decreased to the normal range even in the UW group with ongoing perfusion. The sensitivity of our model in detecting protective effects of the tested solution was confirmed by a negative control group of livers stored in Ringer’s solution at 4°C, yielding an impaired recovery which differed by one magnitude from the three other groups. Within the limits of an in vitro study it is concluded from these results that Celsior may become a suitable alternative for liver preservation and further studies including a transplantation in vivo are strongly encouraged.

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Section: Metabolic Analysismentioning

confidence: 99%

Experimental Liver Preservation with Celsior:A Novel Alternative to University of Wisconsin and Histidine-Tryptophan-α-Ketoglutarate Solutions?

Tolba

Akbar²,

Müller³

et al. 2000

Eur Surg Res

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“…For the first time probably in clinical practice, the ROP was applied to kidneys by Rolles in 1989 (12). Studies comparing the quality of grafts preserved by ROP with those routinely subjected to cold storage or intra-arterial perfusion have been performed on a repetitive basis (12,13). It has been shown that ROP, from many aspects, can be even more beneficial than the customary preservation both for kidneys and for liver.…”

Section: Discussionmentioning

confidence: 99%

Retrograde Oxygen Persufflation of Kidney – Experiment on an Animal

Moláček

Opatrný

Matějka

et al. 2016

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“…It is known that hypothermic perfu sion for 24 h at a high pressure produces histologic changes and reduces renal function after transplantation [5], Experimental preservation of dog kidneys with 0 2-persufflation have shown good biochemical preservation of the renal tissue [7], but such kidneys did not function after retransplan tation [8], Contrary to this, Talbert et al [ 11J demonstrated good func tion of kidneys perfused in situ with oxygen during 3-4 h of complete is chaemia.…”

Section: Discussionmentioning

confidence: 98%

Gaseous Persufflation during Machine Perfusion of Human Kidneys before Transplantation

Flatmark¹,

Slaattelid²,

Woxholt³

1975

Eur Surg Res

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Massive air embolisation (persufflation) of four machine-perfused human kidneys occurred during preservation. This lasted for 60–120 min and caused increasing perfusion resistance. Pressure and flow returned to normal within 2 h after reperfusion. All kidneys showed immediate function after transplantation. Experiments with dog kidneys revealed closure of cortical vessels and increased kidney temperature during persufflation. Cortical flow became normal after 1 h of reperfusion.

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Metabolism of canine kidneys in anaerobic ischemia and in aerobic ischemia by persufflation with gaseous oxygen

Cited by 20 publications

References 24 publications

Experimental Liver Preservation with Celsior:A Novel Alternative to University of Wisconsin and Histidine-Tryptophan-α-Ketoglutarate Solutions?

Experimental Liver Preservation with Celsior:A Novel Alternative to University of Wisconsin and Histidine-Tryptophan-α-Ketoglutarate Solutions?

Retrograde Oxygen Persufflation of Kidney – Experiment on an Animal

Gaseous Persufflation during Machine Perfusion of Human Kidneys before Transplantation

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