Metabolism of canine kidneys in anaerobic ischemia and in aerobic ischemia by persufflation with gaseous oxygen

Isselhard, W.; Berger, M.; Denecke, H. J.; Witte, J; Fischer, J.; Molzberger, H; Freiberg, Christoph; Ammermann, D.; Brunke, M.

doi:10.1007/bf00587833

Cited by 44 publications

(10 citation statements)

References 33 publications

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“…In the first set of experiments, hearts from medium-sized dogs were cannulated at the coronary ostia, flushed with pre-oxygenated normal saline, then persufflated with humidified gaseous carbogen (a mixture of 95% O 2 and 5% CO 2 ). This approach, generally termed anterograde PSF (A-PSF), contrasts with retrograde PSF (R-PSF) which would be tried in the heart [8; 87] and subsequently in the kidney [28; 32]. Hearts maintained at 37°C continued to beat for an average duration of 5.1 hours (2.5–8 hours, range).…”

Section: Whole Organ Persufflationmentioning

confidence: 99%

“…Isselhard and his collaborators published a series of studies that examined the differences between A-PSF and R-PSF [28; 29; 30; 31; 32; 77], which is PSF by delivering the gas in the direction opposite to physiologic flow (starting at the venous end). Historically, the technique of R-PSF also involved the introduction of small, pin-pricks into the surface of the organ – to facilitate gas efflux as illustrated in Figure 1.…”

Section: Whole Organ Persufflationmentioning

confidence: 99%

“…The relative merits of R-PSF versus A-PSF were not clearly established by earlier studies and it was not until the early 1970s that studies were undertaken to directly compare the two methods in a single system (canine kidney) [28; 29; 32]. Isselhard et al developed an in situ ischemia model to study the effects of SCS, A-PSF or R-PSF on canine kidney preservation.…”

Section: Comparison Between Anterograde and Retrograde Persufflationmentioning

confidence: 99%

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Persufflation (or gaseous oxygen perfusion) as a method of organ preservation

et al. 2012

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Improved preservation techniques have the potential to improve transplant outcomes by better maintaining donor organ quality and by making more organs available for allotransplantation. Persufflation, (PSF, gaseous oxygen perfusion) is potentially one such technique that has been studied for over a century in a variety of tissues, but has yet to gain wide acceptance for a number of reasons. A principal barrier is the perception that ex vivo PSF will cause in vivo embolization post-transplant. This review summarizes the extensive published work on heart, liver, kidney, small intestine and pancreas PSF, discusses the differences between anterograde and retrograde PSF and between PSF and other conventional methods of organ preservation (static cold storage, hypothermic machine perfusion). Prospective implications of PSF within the broader field of organ transplantation, and in the specific application with pancreatic islet isolation and transplant are also discussed. Finally, key issues that need to be addressed before PSF becomes a more widely utilized preservation strategy are summarized and discussed.

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Section: Whole Organ Persufflationmentioning

confidence: 99%

Section: Whole Organ Persufflationmentioning

confidence: 99%

Section: Comparison Between Anterograde and Retrograde Persufflationmentioning

confidence: 99%

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Persufflation (or gaseous oxygen perfusion) as a method of organ preservation

et al. 2012

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“…Currently, CS with University of Wisconsin or HTK solution is the clinical gold standard. It is well established that VSOP, when it is applied to kidney and liver grafts, can improve the preservation quality and thereby increase graft viability 27‐32…”

Section: Discussionmentioning

confidence: 99%

Impact of venous systemic oxygen persufflation supplemented with nitric oxide gas on cold-stored, warm ischemia-damaged experimental liver grafts

et al. 2012

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The increasing shortage of donor organs has led to the increasing use of organs from non-heart-beating donors. We aimed to assess the impact of venous systemic oxygen persufflation (VSOP) supplemented with nitric oxide (NO) gas during the cold storage (CS) of warm ischemia (WI)-damaged experimental liver grafts. Rat livers (n ¼ 5 per group) were retrieved after 30 minutes of WI induced by cardiac arrest (the WI group) and were thereafter preserved for 24 hours by CS in histidine tryptophan ketoglutarate solution. During CS, gaseous oxygen was insufflated via the caval vein with 40 ppm NO (the VSOP-NO group) or without NO (the VSOP group). Cold-stored livers without WI served as controls. Liver viability was assessed after the preservation period by normothermic isolated reperfusion for 45 minutes with oxygenated Krebs-Henseleit buffer. After 45 minutes of reperfusion, the VSOP-NO-treated livers showed significantly lower alanine aminotransferase values than the WI-damaged livers (10.2 6 0.2 versus 78.2 6 14.6 IU/L), whereas the control livers showed no differences from the VSOP-NO-treated livers. The mitochondrial enzyme release was lower in the VSOP-NO group (4.0 6 0.7 IU/L) versus the WI group (18.2 6 4.9 IU/L). An increased portal vein pressure was observed throughout reperfusion (45 minutes) in the WI group (21.7 6 0.2 mm Hg) versus the VSOP-NO group (12.2 6 0.8 mm Hg) and the control group (19.9 6 0.4 mm Hg). Furthermore, the NO concentration in the perfusate after 5 minutes of reperfusion was highest in the VSOP-NO group. The release of malondialdehyde into the perfusate was significantly reduced in the VSOP-NO group (0.9 6 0.1 nmol/mL) versus the WI group (31.3 6 5.3 nmol/mL). In conclusion, the resuscitation of livers after 30 minutes of WI to a level comparable to that of nonischemically damaged livers is possible with VSOP supplemented with NO gas. Moreover, the application of VSOP with NO minimizes the extent of injuries caused by oxygen free radicals during preservation.

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“…One alternative for oxygenation that involves retrograde perfusion of gaseous oxygen through the organ is well worth mentioning 71,72 . Persufflation has yielded surprisingly successful results in oxygenating and replenishing ATP supplies, particularly in ischemically injured organs 73– 75 .…”

Section: The Organomatics Platformmentioning

confidence: 99%

Organomatics and organometrics: Novel platforms for long-term whole-organ culture

2014

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Organ culture systems are instrumental as experimental whole-organ models of physiology and disease, as well as preservation modalities facilitating organ replacement therapies such as transplantation. Nevertheless, a coordinated system of machine perfusion components and integrated regulatory control has yet to be fully developed to achieve long-term maintenance of organ function ex vivo. Here we outline current strategies for organ culture, or organomatics, and how these systems can be regulated by means of computational algorithms, or organometrics, to achieve the organ culture platforms anticipated in modern-day biomedicine.

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Metabolism of canine kidneys in anaerobic ischemia and in aerobic ischemia by persufflation with gaseous oxygen

Cited by 44 publications

References 33 publications

Persufflation (or gaseous oxygen perfusion) as a method of organ preservation

Persufflation (or gaseous oxygen perfusion) as a method of organ preservation

Impact of venous systemic oxygen persufflation supplemented with nitric oxide gas on cold-stored, warm ischemia-damaged experimental liver grafts

Organomatics and organometrics: Novel platforms for long-term whole-organ culture

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