Palíková M., J. Mare‰, J. Jirásek : Characteristics of Leukocytes and Thrombocytes of Selected Sturgeon Species from Intensive Breeding. Acta Vet. Brno, 1999, 68: 259-264. Analysis of leukocyte and thrombocyte characteristics in Acipenser baerii, Acipenser stellatus and Huso huso sturgeons at the age of about 200 days kept under intensive breeding conditions is presented. The differential leukocyte count of studied fish species was of lymphocytic character (68.0-73.5% of lymphocytes), and the neutrophilic and eosinophilic granulocytes amounted to 21.8-25.1% and 3.0-4.6%, respectively. Statistically significant (p< 0.05) differences were found between individual sturgeon species in morphometric characteristics of studied cells (i.e. thrombocytes, lymphocytes, neutrophilic and eosinophilic granulocytes). The highest values were found in Acipenser baerii. These results are in good agreement with data on the ploidy in sturgeon species under study. Acipenser baerii, Acipenser stellatus, Huso huso, differential leukocyte count, thrombocytes, lymphocytes, neutrophilic granulocytes, eosinophilic granulocytesThe family Acipenseridae may be divided into two groups with respect to the number of chromosomes and the amount of nuclear DNA. The first group comprises species having a large number of chromosomes (2n = 250). The species A. baerii and A. gueldenstaedti belong to this group. The second group includes, for example, H. huso, A. ruthenus, A. stellatus with lower numbers of chromosomes in their genomes (2n = 118-120) (Vasilyev et al. 1980;Gorshkova et al. 1996;Arefjev and Nikolaev 1991). Several studies have shown correlation between the ploidy in various fish species and the red blood cell size (Arefjev and Nikolaev 1991; Flaj‰hans 1997; Svobodová et al. 1998).The aim of our study was to evaluate interspecies differences in morphological characteristics of leukocytes and thrombocytes in selected sturgeon species (i.e. A. baerii, H. huso and A. stellatus) known for different numbers of chromosomes. Materials and MethodsA total of 10 Acipenser baerii, 10 Huso huso and 5 Acipenser stellatus sturgeons originating from the Fishery Hluboká, Mydlovary farm were sampled (A. baerii in September, A. stellatus a H. huso early in December). All the fish used in the study were clinically healthy without any signs of disease. The fish were kept under intensive breeding conditions in flow-through troughs with controlled environment (water temperature of 20 o C, water oxygen saturation over 80%). Other hydrochemical indicator values assessed in discharge water characterise the environmental conditions suitable for intensive sturgeon culture. The fish were fed complete feeding mixtures. Basic haematological and biochemical parameters have been already published (Jirásek and Mare‰ 1998). Proke‰ et al. (1995ab; studied morphometric characteristics of these sturgeon species with respect to plastic and meristematic signs. Using morphometric characteristics these authors determined the studied species to be pure species.Blood fr...

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The experimental rearing of tench (Tinca tinca) juveniles of two size classes with initial individual weight of 0.8 and 1.2 g was performed into flow-through aquaria connected to recirculation system during a period of 63 days. Three feeds (KARPICO Supreme 7 Ex, ASTA 2, L05/CH) with nutrient content of 37/8, 42/7 and 35/7 (% proteins/ fat) were used in the experiment. Achieved values of production indicators – feed conversion ratio (FCR) and specific growth rate (SGR) ranged from 1.84 to 4.15 and from 0.70 to 1.49 %.d–1, respectively in relationship to the size class and feed used. More favourable values were achieved with the bigger size class regardless to the diet used (FCR 1.84–3.53 and SGR 0.83–1.49 %.d–1) and the best results were achieved when using ASTA 2 feed (2.05 and 1.84 or more precisely 1.36 and 1.49%.d–1).

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The objective of the present study was to determine the values of basic blood indicators and of biochemical plasma indicators of we Is (Si/urlls glanis L.) intensively bred in warmed power station water without natural feeds. The study included 8 feeding experiments using 4 imported (ALMA. BioMar) and 4 domestic pelle ted feeds with different protein (40 -491ft) and fat 18-22'7c) content. The experiment involved 3265 one-year-old she at fish (Su I) of 209 and 295 g average individual weight (in 1996 and 1997. respectively). Analysed were 56 fish of 15 months (1997) and 17 months (1996) Significant changes IP < 0.05) in hematological indicators were found in fish fed the PDl feed mixture and very significant (P < 0.0 I) differences in the Chol a TL values in fish fed BioMar and W 97. These data characterise the adaptation reaction of the internal environment of wels to conditions of intensive aquaculture with warmed water and the nutritional value of used feeds. Intensive aijltacliiture. Sill/rus glanis. wels. pelleted feed. blood indicawl'S.A good adaptability of wels (Silltrlts gianis L.) to various ecological, food and technological conditions enables its farming diversity in various production systems. Besides pond and combined culture, the whole wels production cycle from fry to marketable fish can be run in special facilities with controlled water temperature regime and utilizing commercial feeds (J i n1 s e k et al. 1998).The aim of the experiments performed in 1996 and 1997 was to test the production of twoyear-old wels of lower jndividual body mass in intensive aquaCUlture, The achieved production results are presented in the study of linlsek and r--lareS (1998). i\latcrials and MethodsThe experiments were carried out in the fish farm TisO\'u ICeske f) b:irst\i Mari:inske Lazne PIC). Heated powerplant effluents were used. Water temperature was controlled using the water from the Ohre river. Partly shaded concrete raceways (production volume 3 m', int10w rate 2 l·s·1 ) were utilized for the experiments. The production and physiological effects of --1 imported pelleted feed produced by ALl\IA and BioMar. one domestic pelleted feed PD 2 and 3 experimental diets of own prescriptions I indicated A. Band \V97) were tested in two replicates. The feeds differred in protein (40 -46 '7r) and lipid content (8 -22 '7(). All feeds were produced by extrusion.

KUKAČKA VLADIMÍR, JIRÁSEK JIŘÍ, KOPP RADOVAN, MAREŠ JAN: The utilization of rapeseed expeller for carp (Cyprinus carpio) diets. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 2013, LXI, No. 5, pp. 1289-1295 Production eff ectiveness of rapeseed stamping in the common carp fodder was attested in the controlled conditions of recirculation system with the absence of natural food. 4 isonitrogenous (31.5% NS) and isoenergetic (17.3 MJ GE.kg ) diet with diff erent (35 and 40%) unmodifi ed (URS) and technologically modifi ed (MRS) rapeseed expeller ratio were tested. During the sixty-day feeding trial, benefi cial production eff ect of diet with modifi ed stamping was proved. Diet MRS fi sh were characterized by signifi cantly (P < 0.05) faster length growth. In comparison with fry of MRS variant, they have reached highly signifi cantly (P < 0.01) the higher values of broad -backedness index and signifi cantly (P < 0.05) higher values of body width. Signifi cant values were not detected by individual weight and HSI. Except the haematocrit value, there were no hematologic and biochemical indices infl uenced by feed type. Higher content of fat in the URS was shown at fi sh variant URS/40 by higher fat deposition in hepatopancreas (averagely 30.31%), without statistically signifi cant diff erence compared to other variants. carp fry, growth intensity, feed conversion, haematological parameters Climate changes in the global impact infl uence the sources of basic compound for fi sh feed production -the fi sh-fl our. Also, they infl uence a crop structure of agriculture production with extension of foretime marginally grown crops. Regard to the availability, high consumption and price, there are possibilities of partial reduction of fi sh-fl our in aquafeeds. Namely for the carnivore salmonids and fi sh of prey breeding, the nutrition identifi cation of non-traditional protein sources suitability has prior breeding and economical intent. The development of productively eff ective and aff ordable diet is necessary even for juvenile omnivorous fi sh breeding, including carp. According to TACON and JACKSON (1985), the level of fi sh-fl our substitution in aquafeed depends primarily on the essential fat acids balance and presence of antinutrition substances. In connection with biofuel using program under the EU, the signifi cant growth of oilseed rape (Brasicca napus L.) arisen. Products obtained by industrial manufacturing can be considered as perspective feed ingredients and protein sources for fi sh, as well as the modern technological methods in the processing and the technique of feed production (extrusion).For feeding purposes the oilseed rape is used mainly in extracted grout or stampings. THIELSSEN et al. (2004) added the rape protein concentrate (canola concentrate -CPC) into the feed for rainbow trout (Onchorhynchus mykiss). The best results were reached with 50% presence of CPC in dry matter of feed. The digestibility data of variously processed oilseed rape by see bass carried out i...

Conversion success of pond-reared zander (Sander lucioperca) fry was evaluated using three different converting methods: direct conversion to dry feed (Bio-Optimal Start 0.8 mm; variant A), three–day application of semimoist feeding mixture on the base of fish (variant B) and three-day application of semimoist mixture + three-day co-feeding of semimoist mixture and dry feed (variant C). Two replicates per treatment were applied. These three methods were evaluated regarding specific growth rate (SGR), condition coefficient (K), survival and cannibalism. Mean initial total length (TL) of fish was 35.22 ± 2.15 mm and mean individual weight (w) was 0.31 ± 0.05 g. There were 600 individuals in each circular tank. The initial stock density was 2 fish.l – 1. Fish survival during a 14-day period of rearing ranged from 34.33 % (variant C) to 50.33 % (variant A). Significantly (P < 0.05) better conversion performance was achieved with variants A and B than with the variant C regarding all parameters.

During the First World War, the attitude of Czech politicians to the idea of the existence of an independent state changed fundamentally. T. G. Masaryk, who became the main representative and agent of the efforts for independence, formed, together with his associates the Czech Foreign Committee, later transformed into the Czechoslovak National Council, which sought to win over the representatives of the Entente to the idea of an independent Czech state. The Czechoslovak National Council was gradually recognized in 1918 by the individual powers of the Entente as a provisional government. The fundamental document of the domestic policy was the Declaration of the Czech Deputies of the Imperial Council and Provincial Assemblies, also called the Epiphany Declaration, adopted on 6th January 1918, demanding the independence for its nation. T. G. Masaryk responded to the manifesto of Emperor Charles I on the federalization of the Austrian part of the monarchy, an attempt to save the empire, by solemnly declaring the Czechoslovak independence, which is also known as the Washington Declaration. It already presented the form of the Czechoslovak state –republic. Czechoslovakia was established as an independent state by a revolutionary act of the Czechoslovak National Committee on 28th October 1918, which on this day declared itself the government of the new state, the executor of state power and at the same time a legislative body. Already on 13th November 1918, the National Committee promulgated a Provisional Constitution. Given its provisional nature, the Revolutionary National Assembly had the most important role, to draft and approve the fundamental law of the state. The constitutional basis of the first pre-Munich republic became the Constitutional Charter of 1920. The approval of the constitution represents the culmination of the formation of the Czechoslovak state. The adoption of the Constitutional Charter of the Czechoslovak Republic in February 1920 meant the definitive break-up of the new republic with the constitutional-legal continuity and the tradition of Austrian constitutionality.