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A flat electrophoretic migration velocity profile Is assumed to be characteristic of current capillary zone electrophoresis (CZE) separations. However, this electrophoretic velocity profile may not actually exist In some experiments due to heat generated within the capillary. The actual migration velocity stream within capillary electrophoresis tubes probably has a parabolic profile. Using a parabolic model, we have developed an expression that quantitatively relates the plate height to experimental parameters. The plate height In CZE is a strong function of the capillary radius, the strength of the applied field, and the buffer concentration. The newly derived plate height equation suggests the following: (a) large operating voltages may broaden solute peaks; (b) capillaries should be carefully thermostated; (c) thermostating at elevated temperatures may lead to Improved plate heights; and (d) decreasing the electrolyte concentration In the buffer allows the use of wider capillaries. Wider capillaries, In turn, would permit more convenient operation and large sample volumes.