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GRain is freely available software intended to enable and promote testing of hypotheses with respect to purging and heterogeneity of inbreeding depression. The program is based on a stochastic approach, the gene dropping method, and calculates various coefficients from large and complex pedigrees. GRain calculates, together with the 'classical' inbreeding coefficient, ancestral inbreeding coefficients proposed by Ballou, (1997) J. Hered., 88, 169 and Kalinowski et al., (2000) Conserv. Biol., 14, 1375 as well as an ancestral history coefficient (AHC ), defined here for the first time. AHC is defined as the number that tells how many times during pedigree segregation (gene dropping) a randomly taken allele has been in IBD status. Furthermore, GRain enables testing of heterogeneity and/or purging of inbreeding depression with respect to different founders/ancestors by calculating partial coefficients for all previously obtained coefficients.

Demographic history, current status, and efficiency of the mating strategy were analyzed using the pedigree of Pannon White (PW) rabbits born between 1992 and 2007. Potential accumulation of detrimental effects and loss of genetic diversity were also considered. Calculations and estimates were done most often for rabbits born in 2007, whereas other reference populations (REFPOPXXXX) were specified explicitly. The pedigree contained 4,749 individuals and 580 founders, and its completeness was 82.1% up to 10 and 94.5% up to 5 generations, respectively. Generation intervals through different pathways averaged 1.2 yr. When adjusted to the pedigree completeness, the amount of inbreeding (F(i)) of rabbits was comparable (5.54%) with that of other livestock populations, whereas the 10 (30) founders contributing the most to inbreeding explained a large part of the population inbreeding [i.e., 42.24% (73.18%)]. The ancestral inbreeding coefficient of REFPOP2004 (10.67%) was one-half that of REFPOP2007 (20.66%), showing its strong dependence on pedigree length. Family variance, inbreeding, and realized effective population size were 84.18 (REFPOP2006; this variable could not be calculated for the last year examined), 37.19, and 91.08, respectively. The effective numbers of ancestors, founders, and founder genomes were 48, 26, and 7.33, respectively. Although the circular mating scheme applied was generally effective, the large accumulated reduction in genetic variability indicates the need to revise and improve the current breeding strategy.

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IntroductionA high performance 0.20pm logic technology has been developed with six levels of planarized copper interconnects. 0.15pm transistors (Lg,,,=0.15+0.04pm) are optimized for 1.8V operation to provide high performance with low power-delay products and excellent reliability. Copper has been integrated into the back-end to provide low resistance interconnects. Critical layer pitches for the technology are summarized in Table 1 and enable fabrication of 7.6pm2 6T SRAM cells.Isolation and Transistors CMP planarized shallow trenches with good electrical isolation down to n+/p+ spacings of 0.5pm were fabricated (Fig. 1). Dual gate 0.15pm transistors with 35A physical gate oxides (accumulation t,,=39A measured at Vg=+l .SV) were formed using super steep retrograde channels, shallow extensions and halos, relatively deep source/drain regions and 1 OOnm nitride spacers. CoSi, was selectively formed on the polysilicon gates and source/drain regions with a nominal sheet resistance of 9Wsq. Rapid thermal processing was utilized as much as possible throughout the flow to minimize transient enhanced dopant diffusion.Fig. 2 shows a typical SEM cross-section of a NMOS transistor with a gate length of 0.15pm. Well delineated shallow S/D extensions and the deeper S/D junctions are clearly observed. The saturation drive currents for nominal gate length NMOS and PMOS devices are shown in Fig. 3 . The nominal drive currents are 630pNpm for NMOS and 230pA/ym for PMOS at 1.8V. The off-state leakage currents of these devices are well below the worst case leakage specification of 2nA/pm. The drain induced barrier lowering (DIBL) measured on NMOS and PMOS devices is plotted as a function of Leff in Fig. 4. Good short channel characteristics are maintained down to effective channel lengths of O.1ym. The Vt roll-off for N and P devices in the linear and saturation regions are shown in Fig. 5. The Vt's are 0.44V and -0.46V for Nch and Pch respectively, at a gate length of 0.15pm and the associated subthreshold slopes are less than 90mv/dec. The use of nitrided gate oxides was investigated due to their superior hot carrier reliability. Fig. 6 compares the degradation under hot carrier stress of nitrided oxides to thermal oxides and highlights the improved reliability of NO-annealed oxides. Peak Gms comparable to those from thermal oxides were obtained (Fig. 7). A further advantage afforded by nitrided gate dielectrics is its superior boron blocking properties, Increasing the poly silicon doping in the P+ gate to reduce poly depletion resulted in only a 88mV Vt shift in nitrided oxides (Fig. 8) compared to a 300mV Vt shift in thermal oxides. A significant reduction in the inversion to, is achieved with the higher gate doping, resulting in improved device characteristics. NMOS transistor design focused on minimizing defect enhanced dopant re-distribution such as TED. To this end, the effect of different source/drain implant energies on NMOS transistor performance is shown in Fig. 9. The lower energy implant results in a significantl...

Introduction: Airway management is an integral part of general anaesthesia, which may lead to severe short- and long-term complications. Aim: We assessed whether the application of a checklist for the steps of airway management reduces the number of complications in our institute. Method: In our observational, prospective, controlled study we made a checklist and a data collection sheet. Each airway management was performed for one month without the checklist and then for one month in the possession of the checklist. We evaluated the outcome of airway maneuvers and the occurrence of related early complications before and after the introduction of the checklist. The primary endpoint was the incidence of unexpected difficult airway. The secondary endpoints were difficult intubation, successful first intubation, aspiration, cardiac arrest, post-induction hypotension and desaturation, soft tissues/teeth injuries. Our results were also corrected for factors that affect the risk of complications (urgency of interventions, medical experience). Results: We did not find any difference in the frequency of acute complications before the introduction of the checklist (n = 439) and during the subsequent period (n = 423). At the primary endpoint (7.29% and 6.14%), there was no substantive difference (1.15%, 95% CI: –2.26%–4.56%, p = 0.5). No differences were found regarding the secondary and other endpoints. Following the correction of risk factors, there was no impact of the checklist on the incidence of complications. Conclusion: The introduction of the checklist in itself did not result in a significant change in the risk of short-term complications of airway management in our institution. Orv Hetil. 2019; 160(26): 1025–1035.

In a synthetic closed population of Pannon White rabbits, additive (VA ), dominance (VD ) and permanent environmental (VPe ) variance components as well as doe (bF d ) and litter (bF l ) inbreeding depression were estimated for the number of kits born alive (NBA), number of kits born dead (NBD) and total number of kits born (TNB). The data set consisted of 18,398 kindling records of 3883 does collected from 1992 to 2009. Six models were used to estimate dominance and inbreeding effects. The most complete model estimated VA and VD to contribute 5.5 ± 1.1% and 4.8 ± 2.4%, respectively, to total phenotypic variance (VP ) for NBA; the corresponding values for NBD were 1.9 ± 0.6% and 5.3 ± 2.4%, for TNB, 6.2 ± 1.0% and 8.1 ± 3.2% respectively. These results indicate the presence of considerable VD . Including dominance in the model generally reduced VA and VPe estimates, and had only a very small effect on inbreeding depression estimates. Including inbreeding covariates did not affect estimates of any variance component. A 10% increase in doe inbreeding significantly increased NBD (bF d  = 0.18 ± 0.07), while a 10% increase in litter inbreeding significantly reduced NBA (bF l  = -0.41 ± 0.11) and TNB (bF l  = -0.34 ± 0.10). These findings argue for including dominance effects in models of litter size traits in populations that exhibit significant dominance relationships.

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