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MAASSAB, H. F. (University of Michigan, Ann Arbor), AND J. A. VERONELLI. Characteristics of serially propagated monkey kidney cell cultures with persistent rubella infection. J. Bacteriol. 91:436-441. 1966.-A persistent infection of LLC-MK2 cells with rubella virus has been established and maintained for over 3 years. This "carrier culture," designated as LLC-MK2-RAL, possesses distinct morphological and biological characteristics when compared with the original uninfected LLC-MK2 line. The mechanism of viral persistence has not been entirely elucidated, but available data suggest a regulated infection with transmission of the virus directly from cell to cell or through cell division. Interferon was isolated from RAL (rubella-associated line) culture, which explains partly the wide spectrum of resistance to superinfecting viruses. Amantadine, although inhibiting cultures of LLC-MK2 cells infected with rubella virus, failed to cure the "carrier culture." 100 TCID5o per milliliter of echovirus 11 was added in

Antibodies for rubella virus were detected in human serum and titrated by the indirect method of immunofluorescence; a chronically infected, continuous line of monkey kidney cells was used as antigen. Positive reactions were obtained with serums from convalescent patients or persons who had been exposed to the virus while serums from patients in the acute stage of the disease and those from unexposed individuals were negative

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