Characterization of growth of rubella virus in LLC-MK2 cells

Veronelli, J. A.; Maassab, Hunein F.

doi:10.1007/bf01253851

Cited by 12 publications

(8 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…9, the anniulate lamellae are seen in cross section. X 54,000. the observation that specific immunofluorescence (2,31) is restricted to the cytoplasm and that cytoplasmic but not nuclear inclusions (28,30) are seen in rubella virus-infected cells in culture. The two most prominent changes observed in the infected cells were the appearance of disorganized arrays of "unit" membranes ( Fig.…”

Section: Discussionmentioning

confidence: 94%

“…The course of events following rubella virus infection of cell cultures (22,29,30) and the occurrence of naturally infected carrier cultures (9, 23) have been described. Light microscopy of rubella virus-infected tissue culture cells by use of fixed and stained preparations (28,30) or by techniques employing immunofluorescence (2, 31) indicate the presence of virus material in the cytoplasm without involvement of the nuclei of the cells. Cytological investigation by electron microscopy has been restricted to examination of cell-free extracts and partially purified preparations of virus (16, 21).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Electron Microscopy of Monkey Kidney Cell Cultures Infected with Rubella Virus

Kim

Boatman

1967

J Virol

View full text Add to dashboard Cite

Two rubella virus strains isolated in this laboratory were investigated in terms of their growth in LLC-MK2 cell cultures and their effect on cell morphology. Rubella virus grew readily in LLC-MK2 cells, but cytopathic effects of the virus were not observed in infected cultures. Such infected cultures can be subcultured indefinitely and continue to shed virus. Examination of rubella-infected cell cultures by electron microscopy showed the presence of annulate lamellae in the cytoplasm of 15% of the cells. No changes were evident in the nuclei. These membranous inclusions varied in complexity from parallel arrays of annulate lamellae to large lamellar structures of complex morphology. An occasional cell contained a crystal lattice structure in association with the lamellae. Larger inclusions, consisting of disorganized arrays of "unit" membranes, were also found. Uninfected cells were devoid of annulate lamellae, crystals, and complex membranous inclusions. No viruslike particles were observed in any part of the cells from infected cultures. The significance of the structures observed has not been determined.

show abstract

Section: Discussionmentioning

confidence: 94%

mentioning

confidence: 99%

Electron Microscopy of Monkey Kidney Cell Cultures Infected with Rubella Virus

Kim

Boatman

1967

J Virol

View full text Add to dashboard Cite

show abstract

“…One characteristic of earlier work on rubella grown on tissue cultures was the absence of any noticeable cytopathic effect, although more recent work (e.g., Veronelli and Maassab, 1965) appears to show that cytopathic effects can be produced if a suitable cell line is chosen. The above authors describe eosinophilic cytoplasmic inclusions in their experiments.…”

Section: Discussionmentioning

confidence: 99%

Further observations on inclusion-bearing cells in urinary sediment in infectious diseases

Boyd¹,

Nedelkoska²

1967

Journal of Clinical Pathology

View full text Add to dashboard Cite

SYNOPSIS A study of the cytology of the urinary sediment in 43 patients with known viral diseases has revealed a variety of inclusion-bearing cells in 28.The morphology of the cells suggest that the changes recorded may be due to the viral infections, at least in some instances, bearing in mind the findings of workers quoted in our 1964 report that cellular changes very similar to those induced by virus infections can be initiated by non-viral stimuli. Multinucleate giant cells are occasionally found in chickenpox, measles, herpes simplex infection, and in mumps.The work of Bolande (1959 and1961) stimulated us to examine the cytology of urinary sediment, and some of our preliminary observations have been recorded (Boyd andNedelkoska, 1963 and. These showed that inclusion-bearing cells were noted in 25 of 100 patients consecutively admitted to Ruchill Hospital, the 'positive' cases including five with varicella, two with herpes zoster, the other cases including central nervous system, respiratory, alimentary, and cutaneous conditions for which no viral aetiology was proved. Among the 75 'negative' cases, there were patients with illnesses of accepted viral aetiology, for example, rubella, primary herpes simplex, mumps, infective hepatitis, homologous serum jaundice, poliomyelitis (type 3 strain), and enteritis associated with ECHO virus type 2 infection. Although consideration ought to be given to the study of urinary sediment from normal (i.e. non-infected) children and adults, this investigation has not been pursued in view of the difficulty of defining normality. Appreciation of the existence of latent virus infections and of prolonged excretion of virus in the urine after some infections might make this line of pursuit less appropriate.Our next approach therefore has been to examine a consecutive series of admissions with illnesses of accepted viral aetiology, and the results are presented in this article.'British Council scholar. Present Address: The Clinic for Infectious Diseases, Skopje, Yugoslavia. Received for publication 6 April 1967. MATERIAL AND METHODSA fresh specimen of urine was obtained from the patient on the first morning after admission to hospital and was treated in the same fashion as described in our earlier reports (Boyd andNedelkoska, 1963 and. Since these specimens were obtained usually about 7 a.m. and were processed between 10 and 11 a.m., the routine was established that the specimens were stored in Universal containers (30 ml., 1 fl. oz.) at ward temperature rather than in a refrigerator. The heavy precipitation of urates and phosphates which tended to occur if the latter method was adopted seriously obscured the study of cells. Specimens which showed heavy bacterial growth during this period were discarded as being unsatisfactory; those showing only a few bacteria were retained. None of the patients included in this report belongs to the earlier series.The criteria for 'positive' and 'negative' cells and for 'positive' and 'negative' urine samples are unchanged. RESULTSThe results of...

show abstract

“…On the other hand, exclusion chromatography of rubella virus on Sephadex G-200 has been reported (Veronelli and Maassab, 1965;Schmidt and Lennette, 1966) and…”

Section: Introductionmentioning

confidence: 99%

Purification of infectious rubella virus by gel filtration on sepharose 2B compared to gradient centrifugation in sucrose, sodium metrizoate and metrizamide

Trudel

Marchessault

Payment

1981

Journal of Virological Methods

View full text Add to dashboard Cite

Rubella virus was purified by chromatography on Sepharose 2B after concentration by ultrafiltration on hollow fibers and hydroextraction with PEG 20,000. Yields of 40% infectivity and 70% hemagglutinating activity were routinely obtained. Chromatographic purification was compared to ultracentrifugation in sucrose, metrizamide and sodium metrizoate. Yields were lower in sucrose and metrizamide, while sodium metrizoate reduced the infectivity of the virus below detectable levels. These results demonstrate the advantage of Sepharose 2B for the purification of infectious rubella virus.

show abstract

Characterization of growth of rubella virus in LLC-MK2 cells

Cited by 12 publications

References 10 publications

Electron Microscopy of Monkey Kidney Cell Cultures Infected with Rubella Virus

Electron Microscopy of Monkey Kidney Cell Cultures Infected with Rubella Virus

Further observations on inclusion-bearing cells in urinary sediment in infectious diseases

Purification of infectious rubella virus by gel filtration on sepharose 2B compared to gradient centrifugation in sucrose, sodium metrizoate and metrizamide

Contact Info

Product

Resources

About