Organochlorine pesticides (OCPs) are frequently used worldwide as insecticides, fungicides, herbicides and termiticides and have been associated with a variety of cancers in animal and human studies. In the present study, we examined residues of fourteen OCPs in the serum samples of diagnosed cancer patients and healthy residents of Karachi, Pakistan. A random collection of fasting blood samples was carried out from the donors with informed consent. Serum was separated within 2 h of blood collection and was then subjected to extraction with organic solvents followed by purification with florisil column. The final organic extract of each serum sample was processed with Gas Chromatograph coupled with Electron Capture Detector (GC-ECD). OCPs were detected in 97.59% of the cancer cases and 93.75% of the healthy subjects. Mean concentrations of total OCPs (ΣOCPs) was found elevated in the cancer group (0.606 mg/kg) compared with the control group (0.322 mg/kg). Endosulfan was the highest prevalent OCP with a mean concentration of 0.214 mg/kg in the cancer group and 0.166 mg/kg in the control group. The second most prevalent OCP was 4,4-DDE with a mean concentration of 0.131 mg/kg in the cancer group and 0.019 mg/kg in the control group. Highest level of ΣOCPs was detected in the breast cancer cases (20.411 mg/kg) with a mean level of (2.041 mg/kg). In light of the obtained results and available literature on the subject, it has been concluded that OCPs are positively associated with the risk of various cancers in humans.
Cedrus deodara
(deodar) is practically used, as insect repellent, in the northern areas of Pakistan but no data available therefore this study was conducted to evaluate the effectiveness of deodar oil as an alternate of conventional insecticides against the larval pest stage of mealworm beetle (
Tenebrio molitor
), by feeding method. The aim of the study was to investigate the effectiveness of deodar oil as an alternate of conventional insecticides against the larval pest stage of mealworm beetle (
Tenebrio molitor
), by feeding method. All tested chemicals showed efficacy against the pests. The LC
50
was determined by probit analysis and was found to be 3.41, 0.086 and 0.023% of larvae treated with deodar oil, Carbosulfan and Imidacloprid respectively The LC
50
treated larvae were subjected to the evaluation of protein activity, qualitatively and quantitatively. The protein level in tested insects was enhanced when treated with Imidacloprid, Carbosulfan and deodar oil. The electrophoretic profile of treated insects showed more bands in insects treated with
Cedrus deodara
oil. This electrophoretic profile appeared in 4, 5, 7 and 8 bands for tested chemicals including control. Antifeedant activity was observed for
C. deodara
as larvae were deterred to feed on the food found in the container.
The present study was conducted for the taxonomic, morphometric and limnological assessment of the commercially important ichthyofauna of Sakhakot Stream, Malakand, Pakistan. The study area was divided into three sites along the water course namely Dargai Stream, Sakhakot Stream and Shergarh Stream. Fish samples were collected randomly during January to August 2017 with the help of fish gears. A taxonomic key was designed for the collected fish specimens. Ten fish species were identified belonging to 3 orders and 4 families. Family Cyprinidae was the dominant family with seven representative species while families Siluridae, Nemacheilidae and Mastacembelidae were represented by one species each. Lower mean total length and standard length was recorded in Puntius conchonius (Hamilton, 1822) as 9.2 ± 0.6 cm and 7.3 ± 0.6 cm respectively, while highest mean total length and standard length was recorded in Mastacembelus armatus (Lacepede, 1800) as 28.1 ± 1.7 cm and 15.9 ± 2.4 cm respectively. Mean pH of the water ranged from 6.1 at Shergarh Stream in August to 8.7 at Sakhakot Stream in January. Average temperature range was recorded from 10.9 °C in January at Dargai Stream to 18.7 °C in August at Shergarh Stream. No statistically significant difference was found for temperature (p = 0.96) and pH (p = 0.14) in the three water streams. The present study will provide a baseline for the rearing and enhancement of wild stock of the commercially important ichthyofauna in the field of aquaculture and fisheries.
Escalating pesticide deposition in the aquatic environment is becoming a potential threat to aquatic organisms. Present study was designed to investigate acute toxicity in O. niloticus. the fish were exposed to five different concentrations of malathion, chlorpyrifos and lambda-cyhalothrin (synthetic pyrethroid) pesticides in the laboratory using separate aquaria for each chemical. Mortality ranged from 100 to 0%. The 24 h LC50 (median lethal concentration) for O. niloticus was 5.70, 0.5 and 0.015 ppm for malathion, chlorpyrifos and Lambda-cyhalothrin, respectively. LC50 values obtained after 24 h of exposure indicated that the fish were sensitive to all the three tested pesticides. The mortality rate was directly proportional to the pesticides concentration. The results showed that the lambda- cyhalothrin was found more toxic followed by chlorpyrifos and malathion. The study results suggested that the runoff of organophosphates and synthetic pyrethorid pesticides from nearby agricultural fields to aquatic environment posed health hazards on fish.
Organophosphates and synthetic pyrethroid pesticides are used in residential areas and agriculture, discharge into environment, may cause unpredictable toxicity to non-targeted aquatic organisms. The current study aims to assess the effect of these pesticides on the transaminases, glutamate oxaloacetate transaminase and glutamate pyruvate transaminase enzymes activity in brain and gills of Oreochromis niloticus. Fish were exposed for 24 and 48 h (hours) to the LC0 (non-lethal concentrations) of the malathion, chlorpyrifos and λ-cyhalothrin, respectively. For biochemical assay, a spectrophotometer was used. Glutamate oxaloacetate transaminase activity in brain and gill was significantly (p <0.05) elevated and inhibited, respectively, except in 48 h treated samples. The highest (+258%) and lowest (+8.9%) enhancement in this activity was noted in chlorpyrifos and malathion treated gill samples, respectively. The glutamate pyruvate transaminase activity was significantly (p <0.05) enhanced in all treated samples except in 24 h malathion treated brain. The highest (+340.7%) and lowest (+27%) enhancement in this level was recorded in malathion and chlorpyrifos exposed brain and gills samples. Therefore, it is suggested that both these assessments in fish different organs could be used as a useful biomarker to detect environmental pollution. Thus, the alterations in transaminases activity indicated that the tested pesticides are highly toxic to the health of aquatic organisms and should be periodically and regularly monitored in the environment to reduce pollutant stress on non-target biota, particularly fish.
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