A comparative toxic effect of Cedrus deodara oil on larval protein contents and its behavioral effect on larvae of mealworm beetle (Tenebrio molitor) (Coleoptera: Tenebrionidae)

Abstract

Cedrus deodara (deodar) is practically used, as insect repellent, in the northern areas of Pakistan but no data available therefore this study was conducted to evaluate the effectiveness of deodar oil as an alternate of conventional insecticides against the larval pest stage of mealworm beetle ( Tenebrio molitor ), by feeding method. The aim of the study was to investigate the effectiveness of deodar oil as an alternate of conventional insecticides against the larval pest stage of mealworm beetle ( Tenebrio molitor ), by feeding method. All tested chemicals showed efficacy against the pests. The LC 50 was determined by probit analysis and was found to be 3.41, 0.086 and 0.023% of larvae treated with deodar oil, Carbosulfan and Imidacloprid respectively The LC 50 treated larvae were subjected to the evaluation of protein activity, qualitatively and quantitatively. The protein level in tested insects was enhanced when treated with Imidacloprid, Carbosulfan and deodar oil. The electrophoretic profile of treated insects showed more bands in insects treated with Cedrus deodara oil. This electrophoretic profile appeared in 4, 5, 7 and 8 bands for tested chemicals including control. Antifeedant activity was observed for C. deodara as larvae were deterred to feed on the food found in the container.

Solanum nigrum Fruit Extract Increases Toxicity of Fenitrothion—A Synthetic Insecticide, in the Mealworm Beetle Tenebrio molitor Larvae

“…Protein level in the fat body increased notably after feeding the larvae with FN (300 μg/mL). Other research reports decrease of protein level after application of FN to B. mori [ 39 ] and increase when insecticides from other groups and Deodar oil were applied to T. molitor [ 10 ]. These insects may use different compensatory mechanisms in response to stress, also, different used substances and strategies of their application may develop different reaction.…”

“…The insects can cause losses up to 15% of grains and flour production worldwide [ 3 , 4 , 5 ] and may act as vectors of other pests, e.g., fungi [ 6 ]. For these reasons, the yellow mealworm beetle is a model organism in many studies concerning its susceptibility to plant derivatives [ 7 , 8 , 9 , 10 , 11 ]. In previous reports, we checked the sensitivity of yellow mealworm beetle to Solanum nigrum fruit extract (EXT) and its main glycoalkaloids, given in the diet and in in vitro tests.…”

Review on essential oils, chemical composition, extraction, and utilization of some conifers in Northwestern Himalayas

“…C. deodara EO also exhibited larvicidal (Tenebrio molitor) activity as well as insecticidal properties (Buneri et al, 2017). Other species of C. deodara (C. libani) wood EO and organic extract are also evident to show larvicidal effects (Loizzo et al, 2008).…”

“…On the other hand, himachalene present in the wood EO of C. deodara showed maximum larvicidal activities (LC 50 : 362 μg/ml) against Plutella xylostella (Chaudhary et al, 2011). C. deodara EO also exhibited larvicidal ( Tenebrio molitor ) activity as well as insecticidal properties (Buneri et al, 2017). Other species of C. deodara ( C. libani ) wood EO and organic extract are also evident to show larvicidal effects (Loizzo et al, 2008).…”

Impact of essential oil from plants on migratory activity of Sitophilus granarius and Tenebrio molitor

“…Level of mortality of insects was never higher than 76.0% (essential oil from L. angustifolia in dose of 0.75 µL during more than 96 hours of exposure). Buneri et al (2019) studied efficiency of essential oil from Cedrus deodara (Roxburghe x D. Don) G. Don, 1830 in concentrations of 0.8%, 1.5%, 3.0%, 6.0% and 12.0% as insecticide against larvae of Т. molitor. Death rate of insects after 48 h equaled 17%, 33%, 49%, 64% and 87%, respectively.…”