Background and Aims: Liver is a tissue that is negatively affected by diabetes mellitus (DM). This is due to its central function in the regulation of carbohydrate metabolism. Vanadium salts, which have insulinomimetic effects, have been found to stimulate glycogenesis and glycolysis, as well as to inhibit glycogenolysis and lipolysis. The aim of this study is to evaluate the effect of vanadium sulfate (VS) on biochemical changes in liver tissue of diabetic rats. Methods: Randomly selected 6.0 -6.5 months Swiss Albino rats were separated into two diabetic and two control groups. Group I: non treated animals. Group II: non treated animals orally administered VS (100mg/kg/day for 60 days), group III: STZinduced diabetic animals (65 mg/kg with intraperitoneally) and group IV: STZ-induced diabetic animals administered VS (at same dose and time). Antioxidant enzymes such as catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase, glutathione-S-transferase, as well as alkaline phosphatase, glucose-6-phosphate dehydrogenase, carbonic anhydrase, myeloperoxidase, paraoxonase and lactate dehydrogenase were estimated in liver tissue homogenates of the groups. Results: Liver catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase, glutathione-S-transferase, lactate dehydrogenase, carbonic anhydrase and paraoxonase activities were increased, but alkaline phosphatase, glucose-6-phosphate dehydrogenase and myeloperoxidase activities were decreased in diabetic rats when compared to normal rats. Conclusion: Results show that VS restored altered parameters in the liver tissue and prevented oxidative stress in diabetic rats.
Valproic acid (VPA) is an efficient antiepileptic drug widely used for the treatment of epilepsy and other seizures in both children and adults. It is also reported to have side and toxic effects on many organs and tissues. Vitamin B 6 (Vit B 6 ) is a welldescribed water-soluble vitamin, which has an antioxidant effect. In this study, we aimed to investigate the protective effect of Vit B 6 on VPA-induced brain injury.Male Sprague-Dawley rats were divided into four groups. Group I, control animals;Group II, Vit B 6 (50 mg/kg/day) given rats; Group III, VPA (500 mg/kg/day) given rats; Group IV, VPA and Vit B 6 given rats at same dose and time. VPA and Vit B 6 were administered intraperitoneally and orally, respectively, for 7 days. At the end of the experiments, the rats were sacrificed and brain tissues were taken. Protein carbonyl and sialic acid levels, xanthine oxidase, adenosine deaminase, acetylcholine esterase, lactate dehydrogenase, myeloperoxidase activities, total oxidant status, and reactive oxygen species levels were found to be increased, while glutathione and total antioxidant capacity levels, catalase, superoxide dismutase, glutathione-Stransferase, paraoxonase, and glutathione reductase activities were found to be decreased in the VPA group. Administration of Vit B 6 reversed these defects in the VPA group. These findings indicate that Vit B 6 has a protective effect on VPAinduced brain damage.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.