Selective precipitation of plasmid with a water‐soluble cationic surfactant

Ataci, Nese; Correia, Isabell; Arisan, Inci; Marcos, João Carlos

doi:10.1002/pat.1245

Cited by 2 publications

(3 citation statements)

References 12 publications

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“…This topic has gained more and more interest because of its involvement either for plasmid purification 12 or in gene therapy processes. 13 Indeed, gene delivery has been developed to modify or regulate gene sequences and to treat various diseases.…”

Section: ■ Introductionmentioning

confidence: 99%

Histidine-Based Lipopeptides Enhance Cleavage of Nucleic Acids: Interactions with DNA and Hydrolytic Properties

2015

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Interaction studies and cleavage activity experiments were carried out between plasmid DNA and a series of histidine-based lipopeptides. Specific fluorescent probes (ethidium bromide, Hoechst 33342, and pyrene) were used to monitor intercalation, minor groove binding, and self-assembly of lipopeptides, respectively. Association between DNA and lipopeptides was thus evidenced, highlighting the importance of both histidine and hydrophobic tail in the interaction process. DNA cleavage in the presence of lipopeptides was then detected by gel electrophoresis and quantified, showing the importance of histidine and the involvement of its side-chain imidazole in the hydrolysis mechanism. These systems could then be developed as synthetic nucleases while raising concern of introducing histidine in the design of lipopeptide-based transfection vectors.

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Section: ■ Introductionmentioning

confidence: 99%

Histidine-Based Lipopeptides Enhance Cleavage of Nucleic Acids: Interactions with DNA and Hydrolytic Properties

2015

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“…In our previous paper [15], it was found that N, N', N'-Polyethylene (10)-N tallow-1,3 di amine propane (PEG-10 tallow) cationic surfactant is usable for the selective precipitation of plasmid DNA directly from alkaline lysate. Cationic PEG-10 tallow has long-chain (C 12 -C 18 ) tertiary amines combined with 10 moles poly (oxyethylene) (POE) [15,16].…”

Section: Introductionmentioning

confidence: 99%

“…Cationic PEG-10 tallow has long-chain (C 12 -C 18 ) tertiary amines combined with 10 moles poly (oxyethylene) (POE) [15,16]. However, no reports appear available on the CMC of PEG-10 tallow under different conditions.…”

Section: Introductionmentioning

confidence: 99%

Determination of Critical Micel Concentration of PEG-10 Tallow Propane Amine: Effects of Salt and pH

Ataci¹,

Saraç²

2014

AJAC

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Most applications of surfactants depend on formation and characteristics of micelle. In this study we measured critical micelle concentration (CMC) of cationic surfactant N, N', N'-polyethylene (10)-N tallow-1, 3-di amine propane (PEG-10 tallow) in aqua solution by using electrical conductivity and surface tension methods. The CMC value of PEG-10 tallow with two different methods was found very close to 1.40 × 10 −3 M with electrical conductivity, and 1.41 × 10 −3 M with surface tension. It also investigated the effect of inorganic salt (NaCl) and pH on micellization of cationic surfactant PEG-10 tallow. With increasing addition of NaCl, formation of micelle structure resulted in a higher CMC. However, at low salt concentrations smaller CMC value was observed comparing the critical micelle concentration of individual PEG-10 tallow. When it comes to the effect of changing pH in CMC of the PEG-10 tallow , it was clearly found that micelle formation or CMC of surfactant was independent on alkaline pH (between 6 and 10), but it was dependent on acidic pH (below 5).

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Selective precipitation of plasmid with a water‐soluble cationic surfactant

Cited by 2 publications

References 12 publications

Histidine-Based Lipopeptides Enhance Cleavage of Nucleic Acids: Interactions with DNA and Hydrolytic Properties

Histidine-Based Lipopeptides Enhance Cleavage of Nucleic Acids: Interactions with DNA and Hydrolytic Properties

Determination of Critical Micel Concentration of PEG-10 Tallow Propane Amine: Effects of Salt and pH

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