Effect of vitamin B₆on brain damage in valproic acid induced toxicity

Abstract

Valproic acid (VPA) is an efficient antiepileptic drug widely used for the treatment of epilepsy and other seizures in both children and adults. It is also reported to have side and toxic effects on many organs and tissues. Vitamin B 6 (Vit B 6 ) is a welldescribed water-soluble vitamin, which has an antioxidant effect. In this study, we aimed to investigate the protective effect of Vit B 6 on VPA-induced brain injury.Male Sprague-Dawley rats were divided into four groups. Group I, control animals;Group II, Vit B 6 (50 mg/kg/day) given rats; Group III, VPA (500 mg/kg/day) given rats; Group IV, VPA and Vit B 6 given rats at same dose and time. VPA and Vit B 6 were administered intraperitoneally and orally, respectively, for 7 days. At the end of the experiments, the rats were sacrificed and brain tissues were taken. Protein carbonyl and sialic acid levels, xanthine oxidase, adenosine deaminase, acetylcholine esterase, lactate dehydrogenase, myeloperoxidase activities, total oxidant status, and reactive oxygen species levels were found to be increased, while glutathione and total antioxidant capacity levels, catalase, superoxide dismutase, glutathione-Stransferase, paraoxonase, and glutathione reductase activities were found to be decreased in the VPA group. Administration of Vit B 6 reversed these defects in the VPA group. These findings indicate that Vit B 6 has a protective effect on VPAinduced brain damage.

Reversal of Valproate‐Induced Major Salivary Gland Changes By Moringa Oleifera Extract in Rats

“…Higher levels of SA in tissues are often associated with increased ROS levels [43,44] . Previous research has shown that valproate administration increases SA levels in the brain tissue of rats [27] . In this study, the administration of moringa extract significantly reduced the SA levels that had been elevated by VA.…”

“…[43,44] Previous research has shown that valproate administration increases SA levels in the brain tissue of rats. [27] In this study, the administration of moringa extract significantly reduced the SA levels that had been elevated by VA. This reduction is likely due to the antioxidant properties of the compounds in moringa, which helped scavenge ROS species and mitigate the increase in SA levels induced by VA.…”

“…The decrease in CAT activity was not significant. In previous studies, the SOD and CAT activities of various rat tissues such as lung, [25] brain, [26,27] lens, [23] liver [28] and pancreatic tissues [22] have been reported to be significantly lower in VA‐treated groups compared to control groups. In parallel with the present findings, VA‐induced liver toxicity caused decreased SOD activity in the VA group, no significant change was detected in CAT activity [29] .…”

Purification and characterization of thioredoxin reductase enzyme from commercial Spirulina platensis tablets by affinity chromatography and investigation of the effects of some chemicals and drugs on enzyme activity

“…Turkyılmaz et al observed that vitamin B 6 has a protective effect on valproic acid-induced brain damage. 36 Vitamin C is used for the prevention and treatment of the common cold, mental illnesses, infertility, cancers, and AIDS. It is a cofactor that has an important role in many biological processes.…”

The protective effect of vitamin U on pentylenetetrazole‐induced brain damage in rats

“…In addition to that, Raukas et al [ 58 ] reported that they found elevated mitochondrial OSI in brain tissues of Alzheimer's disease patients. Vit U, whose antioxidant activity has been proven on many toxicity models, [ 59,60 ] showed reversing effect on the distorted activities of antioxidants and oxidant levels of the brain tissues of the PTZ administered group.…”