Abstract. Ratnani DA, Junitha IK, Kriswiyanti E, Dhana IN. 2021. The ethnobotany of Ngusaba ceremonial plant utilization by Tenganan Pegringsingan community in Karangasem, Bali, Indonesia. Biodiversitas 22: 2078-2087. Tenganan Pegringsingan is an ancient village in Bali, Indonesia, which often performs several ceremonies with high intensity. One of them is the Ngusaba ceremony, where many plants are utilized both in species and quantity. Hence, this study aimed to identify the species, family, local names, sources, and parts of plants, used for Ngusaba ceremonies by the Tenganan Pegringsingan community including the Index of Cultural Significance (ICS). Data analysis was qualitative and quantitative. Furthermore, the qualitative method was used to obtain data on the plants’ local names, while snowball sampling was applied to select key informants through in-depth interviews and moderate participation. The results showed that the 130 species distributed in 56 families mostly belonging to the purchased source (34.61%). The Poaceae is the largest family, while the most widely used part of the plant is the leaf. Based on the ICS analysis results, a range of 2-114 values was obtained. The highest value is Base (Piper betle L.) and kangkung (Ipomoea batatas L.) as lowest.
This study aims to determine the morphological character of white, red and black rice as a differentiator ofrice varieties. This research uses 10 rice samples which are 6 local rice of Kupang, 2 varieties of local superior riceand 2 Italian rice. The method used is direct observation of both qualitative and quantitative characteristics of rice.Long grain size of rice is a character that can indicate the distinguishing of local rice, domestic superior and ricefrom abroad. Character of rice morphology is a character very easily observed in knowing the difference of ricevarieties. Character of rice morphology are easily characters observed in knowing the difference of rice varieties.Characters difference can be used to determine the adulteration of rice mixed.
Berdasarkan data sejarah dan arkeologis, masyarakat Bali sekarang ini merupakan hasil perkembangan sejarah zaman pra-sejarah. Masyarakat Bali kuno yang masih memiliki tradisi zaman pra-sejarah disebut masyarakat Bali Aga atau Bali Mula. Pada umumnya masyarakat Bali Aga menempati daerah pegunungan seperti desa Tenganan, Terunyan, Sembiran dan Sidatapa, sedangkan masarakat Bali lainnya disebut masyarakat bali Dataran yan tinggal di kota-kota dan daerah pantai. Sebanyak delapan penanda genetik mikrosatelit autosom (D2S1338, D3S1358, D5S818, D7S820, D11S1984, D13S317, D16S539 dan D21S11) digunakan untuk menentukan variasi alel yang tersebar pada masyarakat desa Bali Aga Sembiran kabupaten Buleleng Bali untuk kepentingan forensik. Dalam penelitian ini ditemukan sebanyak 46 ragam alel dari 8 lokus yang digunakan, ragam alel perlokus berkisar antara 3 pada lokus D5S818 sampai 9 alel pada lokus D11S1984. Nilai kemampuan pembeda (power of discrimination/PD) tertinggi ditemukan pada lokus D11S1984 (0,9394) diikuti oleh D21S11(0,8922), D16S539 (0,8915), D13S317 (0,8602), D7S820 (0,8398), D3S1358 (0,8014), D2S1338 (0,5518) dan D5S818 (0,0143). Hasil ini menunjukkan bahwa lokus D5S818 tidak baik digunakan dalam analisis DNA untuk kepentingan forensik pada masyarakat Bali Aga desa Sembiran.
This research was conducted to know alleles variation and power of discrimination of four pair of primer: DYS19, DYS390, DYS393 and DYS395 of male Pande clan. Epithelial cell mucus was collected from 59 male of Gainer, Klungkung, Badung regencies and Denpasar city. Phenol chloroform method was used to extraction DNA from epithel cells sample. Four primer pair was used to amplify DNA samples in PCR machine on 52-55oC annealing temperature. Amplicon were running on PAGE 10% and visualized with silver nitrate staining. The DNA typing were conducted to determinate of alleles size of amplicon with plotting migration distance of amplicon on semi-log pepper. Genetic diversity and power of discrimination was calculated used Microsoft Exell program. The result of this research showed that 29 alleles were found range of 6-9 with mean 7.25 per locus. The genetic diversity is high category (0.739 ± 0.003, the highest diversity on DYS 390 locus is 0.809 ± 0,004 followed by DYS395, DYS393 are 0,793 ± 0.004 and 0,720 ± 0.005 and the small one is 0.633 ± 0.003 on DYS19 locus. The power of discrimination (PD) of all loci are high category with average value is 0.892, because of / for this reason all of loci usfulnes for forensic purpose on pande clan in Bali.
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