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In order to evaluate the exposure of the northern India rural population to polyaromatic hydrocarbon (PAH) inhalation, indoor pollution was assessed by collecting and analyzing the respirable particulate matter PM2.5 and PM10 in several homes of the village Bhithauli near Lucknow, UP. The home selection was determined by a survey. Given the nature of biomass used for cooking, homes were divided into two groups, one using all kinds of biomass and the second type using plant materials only. Indoor mean concentrations of PM2.5 and associated PAHs during cooking ranged from 1.19±0.29 to 2.38±0.35 and 6.21±1.54 to 12.43±1.15 μg/m3, respectively. Similarly, PM10 and total PAHs were in the range of 3.95±1.21 to 8.81±0.78 and 7.75±1.42 to 15.77±1.05 μg/m3, respectively. The pollutant levels during cooking were significantly higher compared to the noncooking period. The study confirmed that indoor pollution depends on the kind of biomass fuel used for cooking.

A large variety of ornamental and decorative items are manufactured from bone waste by various unorganized sectors in India. An initial survey indicated that workers were exposed at various phases of final product. The subjects (12 industrial units) were tested for total suspended particulate matter (TSPM), particulate matter <10 microm (PM(10)), and particulate matter <2.5 microm (PM(2.5)). Prevalent levels of TSPM ranged between 2.90 and 5.89 mg m(-3). Respirable fractions of occupational dust as PM(10) and PM(2.5) were found in the range of 0.30-2.08 and 0.26-0.50 mg m(-3), respectively. Cytotoxicity study was conducted using hemolysis as a sensitive marker. In an in vitro study, rat RBCs were exposed to the concentration of 25-1,000 microg/ml for 15-120 min. A considerable variation was observed in the hemolytic activity of samples collected from different areas. At 500 microg/ml concentration, the hemolytic activity (12 h) was found to be in the range of 18-25%. Due to limitation in sample mass of respirable fractions, only one concentration (100 microg/ml/2 h) was used for comparative study on hemolysis of RBCs caused by PM(10) and PM(2.5). Interestingly, the hemolytic activity was more at PM(2.5) than PM(10) and TSPM. These results suggest that the respirable particles are capable of reaching deep into the respiratory system. The finding is significant notably when there are no standards available in occupationally exposed populations. This is the first such study. Data could be of importance to policy makers and regulators.

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