Identifying the sex of a bird is important to ensure successful breeding strategies and effective conservation programs. Sex may be identified from the intron size of the CHD1 gene located on the avian sex chromosomes Z and W. However, because of the great nucleotide diversity across different avian species, no given intron is in widespread use without ambiguous results. Complicated modifications of the reaction condition are required to suit different species. Two CHD1 introns were used with a unified reaction condition in this study to simplify the procedure. Consequently, genders of 73 avian species covering 19 families were successfully identified based on this two-intron approach. This means the ability to sex a wider range of avian species using a simplified procedure, greatly assisting in population management at zoos. Zoo Biol 26:425-431, 2007. (c) 2007 Wiley-Liss, Inc.
Molecular sexing of the diversified avian family Strigidae is difficult. Sex identification using the intron length difference between W and Z chromosomal CHD1 genes, as visualized by agarose gel electrophoreses, often produces ambiguous results. Here we describe a simple method for sexing a variety of Strigidae species using oligonucleotide microarrays, on which several sex-specific probes operated complementarily or in concert. The sex of 8 owl species was identified clearly on the microarrays through sequence recognition. This sequence-directed method can be easily applied to a wider range of Strigidae species.
Sex determination of birds is important to ensure successful breeding strategies, especially for endangered species. Most birds are vulnerable to stress during handling, however, so obtaining a sufficient amount of genomic DNA (gDNA) while causing the least amount of harm is a critical issue. Avian gender can be determined based on different CHD1 gene intron sizes in W and Z sex chromosomes. We have compared various specimen sources and have found that the rachis segment of a feather is a good DNA source for determining sex. This indicates that plucking the whole feather is not necessary; a cut feather including the rachis is a superior method because it decreases stress on the examined birds and is accomplished easily.
The aim of this study was to investigate the immediate effect of wearing the functional insoles with different slopes of forefoot wedges on postural stability in young adults during quiet stance. In this study, the functional insole was composed of a forefoot wedge and a medial arch support. Twelve healthy young adults (six males and six females) participated. Each subject wore sneakers with and without functional insole and stood as still as possible on a force plate with feet together, arms by side and head facing ahead for 60[Formula: see text]s, while eyes open and eyes closed, respectively. The functional insole was applied in the random sequence of no insole, wearing insole with a medial arch and a four-degree forefoot wedge, as well as wearing insole with a medial arch and an eight-degree forefoot wedge. The sway areas as well as the maximal excursions of the center of pressure (COP) in anterior–posterior (AP) and medial–lateral (ML) directions were used to evaluate the static postural stability. During stance with feet together and eyes closed, the sway area and maximal excursion of the COP in the AP direction were significantly decreased when wearing an eight-degree forefoot wedge functional insole. Since the reduced displacements of the COP indicated better postural control, it was suggested that the functional insole with an eight-degree forefoot wedge and a medial arch support might be beneficial to improve the postural stability in patients with impaired balance control, especially for whom having high risk of forward falls.
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