How the biochemical machinery evolved from simple precursors is an open question. Here we show that ribonucleotides and amino acids condense to peptidyl RNAs in the absence of enzymes under conditions established for genetic copying. Untemplated formation of RNA strands that can encode genetic information, formation of peptidyl chains linked to RNA, and formation of the cofactors NAD+, FAD, and ATP all occur under the same conditions. In the peptidyl RNAs, the peptide chains are phosphoramidate-linked to a ribonucleotide. Peptidyl RNAs with long peptide chains were selected from an initial pool when a lipophilic phase simulating the interior of membranes was offered, and free peptides were released upon acidification. Our results show that key molecules of genetics, catalysis, and metabolism can emerge under the same conditions, without a mineral surface, without an enzyme, and without the need for chemical pre-activation.
Template-directed incorporation of nucleotides at the terminus of a growing complementary strand is the basis of replication. For RNA, this process can occur in the absence of enzymes, if the ribonucleotides are first converted to an active species with a leaving group. Thus far, the activation required a separate chemical step, complicating prebiotically plausible scenarios. Here we show that a combination of a carbodiimide and an organocatalyst induces near-quantitative incorporation of any of the four ribonucleotides. Upon in situ activation, adenosine monophosphate was found to also form oligomers in aqueous solution. So, both de novo strand formation and sequence-specific copying can occur without an artificial synthetic step.
In this paper, we present the design and analysis of an adaptive cost-effective discrete multitone transponder (DMT) using direct detection (DD) suitable for data center interconnections. Levin Campello margin adaptive (LC-MA) algorithm is applied to the transponder digital signal processing modules to enhance fiber chromatic dispersion (CD) resilience, while achieving highdata rate transmission. The bit error rate (BER) performance and the rate/distance adaptive capabilities of the proposed transponder have been numerically analyzed and compared to bandwidth variable uniform loading, taking into account the transmission impairments at the varying of the fiber length. Specifically, the performance of the designed transponder has been assessed from 20 to 112 Gb/s, extending the achievable reach at 50 Gb/s beyond 80 km of standard single mode fiber (SSMF). The numerical simulations have been compared with experimental results, evidencing good agreement in presence of transmission impairments.
All known forms of life use RNA-mediated polypeptide synthesis to produce the proteins encoded in their genes. Because the principal parts of the translational machinery consist of RNA, it is likely that peptide synthesis was achieved early in the prebiotic evolution of an RNA-dominated molecular world. How RNA attracted amino acids and then induced peptide formation in the absence of enzymes has been unclear. Herein, we show that covalent capture of an amino acid as a phosphoramidate favors peptide formation. Peptide coupling is a robust process that occurs with different condensation agents. Kinetics show that covalent capture can accelerate chain growth over oligomerization of the free amino acid by at least one order of magnitude, so that there is no need for enzymatic catalysis for peptide synthesis to begin. Peptide chain growth was also observed on phosphate-terminated RNA strands. Peptide coupling promoted by ribonucleotides or ribonucleotide residues may have been an important transitional form of peptide synthesis that set in when amino acids were first captured by RNA.
A method for the solution-phase synthesis of branched oligonucleotides with tetrahedral or pseudo-octahedral geometry is described that involves the coupling of 3'-H-phosphonates of protected dinucleoside phosphates and organic core molecules. The dimer building blocks are produced by a synthesis that requires no chromatographic purification and that produces the dimer H-phosphonates in up to 44% yield in less than three days of laboratory work. A total of seven different branched hybrids were prepared, including a new hybrid of the sequence (CG)(4)TBA, where TBA stands for tetrakis(p-hydroxybiphenyl)adamantane that assembles into a material from micromolar aqueous solution upon addition of MgCl(2).
We present results from the first field-trial of a quantum-secured DWDM transmission system, in which quantum key distribution (QKD) is combined with 4 × 10 Gb/s encrypted data and transmitted simultaneously over 26 km of field installed fiber. QKD is used to frequently refresh the key for AES-256 encryption of the 10 Gb/s data traffic. Scalability to over 40 DWDM channels is analyzed.
In order to realize probabilistically shaped signaling within the probabilistic amplitude shaping (PAS) framework, a shaping device outputs sequences that follow a certain nonuniform distribution. In case of constant-composition (CC) distribution matching (CCDM), the sequences differ only in the ordering of their constituent symbols, whereas the number of occurrences of each symbol is constant in every output block. Recent results by Amari et al. have shown that the CCDM block length can have a considerable impact on the effective signal-to-noise ratio (SNR) after fiber transmission. So far, no explanation for this behavior has been presented. Furthermore, the block-length dependence of the SNR seems not to be fully aligned with previous results in the literature. This paper is devoted to a detailed analysis of the nonlinear fiber interactions for CC sequences. We confirm in fiber simulations the inverse proportionality of SNR with CCDM block length and present two explanations. The first one, which only holds in the short-length regime, is based on how two-dimensional symbols are generated from shaped amplitudes in the PAS framework. The second, more general explanation relates to an induced shuffling within a sequence, or equivalently a limited concentration of identical symbols, that is an inherent property for short CC blocks, yet not necessarily present in case of long blocks. This temporal property results in weaker nonlinear interactions, and thus higher SNR, for short CC sequences. For a typical multi-span fiber setup, the SNR difference is numerically demonstrated to be up to 0.7 dB. Finally, we evaluate a heuristic figure of merit that captures the number of runs of identical symbols in a concatenation of several CC sequences. For moderate block lengths up to approximately 100 symbols, this metric suggests that limiting the number identicalsymbol runs can be beneficial for reducing fiber nonlinearities and thus, for increasing SNR.
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