Bacterial wilt (BW), caused by Pseudomonas solanacearum E.F. Smith, is one of the most destructive disease of tomato (Lycopersicon esculentum Mill.) in the tropics. Twenty tomato lines/accessions previously identified as BW-resistant were evaluated for BW reaction in fields providing high disease pressure at Subang, Indonesia; Los Baños, Philippines; Malaysian Agricultural Research and Development Institute (MARDI), Kuala Lumpur; Asian Vegetable Research and Development Center (AVRDC), Taiwan; and Taiwan Seed Improvement and Propagation Service (TSIPS). Entries also were tested in the greenhouse at the AVRDC with a P. solanacearum strain from Taiwan (Pss4) using a drench inoculation method. Objectives of the study were to identify stable sources of BW resistance for southeast Asian tomato breeding programs, and to determine the correlation between field and greenhouse reactions. Mean entry survival was 21.6% at Subang, 31.9% at Los Baños, 76.7% at the AVRDC, 93.6% at Malaysia, and 93.3% at TSIPS, indicating that most entries were resistant at MARDI and the Taiwan locations but susceptible at Subang and Los Baños. L285 (mean survival = 83.8%) and CRA 84-58-1 (mean survival = 79.4%) were the most resistant entries in the field trials. Mean survival (70.1%) of CRA 66-derived entries was significantly better than the mean of entries with resistance derived from UPCA 1169 or UPCA 1169 plus `Venus' or `Saturn'. Mean survival of AVRDC entries bred in the 1980s (59.4%) was significantly greater than mean survival of AVRDC lines bred in the 1970s (45.7%). The correlation between entry BW percent survival averaged over the five field trials and entry means from drench inoculation in the greenhouse was highly significant (r = 0.70), suggesting that the drench inoculation method is effective in selection for BW resistance.

Bacterial wilt, caused by Ralstonia solanacearum, is a major constraint to tomato production in the tropics and subtropics. Most bacterial wilt-resistant tomato cultivars have not shown consistently high resistance levels over locations. The objective of this study was to determine whether combining resistance derived from different sources would result in F1 progenies with resistance greater than that of the parents. Five bacterial wilt-resistant tomato lines or accessions (CL5915, L285, CRA84, H7997, and GA219), each derived from different resistance sources, and a susceptible processing tomato line (UC204A) were crossed in all combinations without reciprocals. Parents, F1 progenies, and F2 progenies were evaluated in greenhouses at three locations (Taiwan, Philippines, and Indonesia) for percent survival 6 weeks after drench inoculation with virulent local strains of R. solanacearum. Percent survival means over locations were 17.4 to 83.0 for parents and F1 progeny and 16.2 to 75.0 for parents and F2 progeny. The percent survival means over locations of L285 × H7997 were highest among crosses in the F1 (83.0) and F2 (75.0) generations but were not significantly greater than that of H7997. Highly significant mean squares were found in the F1 and F2 progenies for general combining ability (GCA) and GCA × locations. Positive GCA effects over locations were detected for H7997, CRA84, and L285, indicating that progeny with those lines as parents showed bacterial wilt resistance that was greater than the average of all crosses. Only H7997, however, had positive GCA effects estimates at each location for each generation, and its GCA effects estimates over locations were significantly greater than those of the other parents in the F1 and F2 progenies. Among this set of parents, H7997 is the best source to develop bacterial wilt-resistant progeny. We did not observe statistically significant increases in resistance by combining different resistance sources. However, the presence of large GCA variances suggests that hybridization of parents that have high GCA for bacterial wilt resistance, such as H7997, CRA84, or L285, followed by selection in segregating populations might yield inbred progeny with resistance greater than that of the parents.

Plant growth promoting rhizobacteria (PGPR) application in combination with other antagonist microbes as biopesticide have been considered in many crops. Our research was conducted to evaluate the efficacy of these useful combinations with the carrying agent for growth promotion, thus lowering white rust incidence in chrysanthemum production. The experiment was carried out at three cooperative farmer sites located in Cipanas, Cianjur, West Java, Indonesia from January to December 2016. The production process was arranged in a paired treatment; a combination of PGPR and antagonist fungi (without supplemental chemical fertilizers and fungicide), furtherly called biofungicide and common farmer practices. The results showed that the application of biofungicide promoted equal plant growth quality as common practices. White rust incidency was lower at biofungicide treatment sites, thus increased the markertable flowers quantity. The production cost was considered more efficient in biofungicide sites, due to cheaper price of biofungicide than chemical fertilizers and fungicide. The increase of marketable stalks and cost efficiency led to an increase of net income of biofungicide based production as also viewed from higher Revenue Cost Ratio (R/C) than common farmer practices.

<p>Consumer demands on safe agricultural products have made the shifting of the production system to be more environmental friendly. An attempt to reduce or totally substitute chemical fertilizers and pesticides on agricultural production process was through the utilization of potential microbes. The purpose of the study was to provide information on potential microbial species that can be used as active ingredients of biofertilizers and biopesticides. The mechanisms of action have been studied, both directly and indirectly, in protecting the plant from pest and disease attacks. Several of these microbes also functioned as decomposer that might improve soil characteristic and nutrient availability for the crops. The Indonesian Agency for Agricultural Research and Development for has released formulated biopesticides and bio fertilizers with the active ingredients isolated from agricultural production centers. The application of these biopesticides and biofertilizers have been effectively controlled important diseases in horticultural crops, i.e. Bio Nutri-V could suppress white rust disease (Puccinia horina Henn) 32.15% in chrysanthemum and increased 25% and 34% harvestable products in chrysanthemum and potato, respectively, compared with synthetic fungicide. The utilization of biopesticides and biofertilizers is expected to improve the competitiveness of national agricultural commodities by utilizing natural resources to support highly competitive and sustainable agricultural industries.</p><p>Keywords: Microbes, biofertilizer, biopesticide, vegetable, ornamentals, horticulture.</p><p> </p><p><strong>Abstrak</strong></p><p>Tuntutan konsumen terhadap keamanan produk pertanian menuntut pula perlunya proses produksi dilakukan secara ramah lingkungan. Salah satu upaya untuk mengurangi atau mensubstitusi penggunaan pupuk dan pestisida kimia sintetik ialah memanfaatkan mikroba. Makalah ini membahas spesies mikroba yang berpotensi dan dapat dijadikan sebagai bahan aktif pupuk dan pestisida hayati. Berbagai spesies mikroba dari kelompok cendawan dan bakteri telah berhasil diisolasi dan dievaluasi keefektifannya sebagai bahan aktif pupuk dan pestisida hayati yang efektif. Mikroba pemacu pertumbuhan tanaman dengan mekanisme langsung maupun tidak langsung mampu menginduksi pertumbuhan tanaman dan beberapa mikroba juga berfungsi sebagai dekomposer, sehingga membantu penyediaan unsur hara bagi tanaman. Badan Litbang Pertanian telah menghasilkan beberapa formulasi pupuk hayati dan biopestisida dengan bahan aktif mikrobe yang diisolasi dari sentra produksi pertanian. Aplikasi pupuk dan pestisida hayati tersebut efektif mengendalikan penyakit penting tanaman hias, seperti Bio Nutri- V dapat menekan perkembangan penyakit karat putih (Puccinia horina Henn) pada krisan 32,2% dan mempertahankan hasil panen kentang dan krisan masing-masing 25% dan 34% dibandingkan dengan aplikasi fungisida kimia sintetik. Pengembangan pupuk dan pestisida hayati yang dihasilkan diharapkan dapat meningkatkan daya saing komoditas pertanian melalui sistem produksi ramah lingkungan dengan memanfaatkan sumber daya alam secara optimal guna mendukung industri pertanian berdaya saing dan berkelanjutan.</p><p>Kata kunci: Mikroba, pupuk hayati, biopestisida, sayuran, tanaman hias, hortikultura.</p>

Gladiol (Gladiolus hybridus L.) merupakan komoditas tanaman hias yang mempunyai prospek pengembangan yang cukup cerah untuk memenuhi kebutuhan dalam negeri maupun ekspor. Salah satu penyakit utama yang menyerang tanaman tersebut ialah layu yang disebabkan oleh Fusarium oxysporum f. sp. gladioli. Di Indonesia, kehilangan  hasil akibat serangan patogen tersebut hampir mencapai 100%. Tujuan penelitian ialah mengendalikan F. oxysporum f. sp. gladioli serta mendorong pertumbuhan tunas subang gladiol melalui  pengasapan dan aplikasi biopestisida. Penelitian dilaksanakan di Rumah Kaca Balai Penelitian Tanaman Hias Segunung (1.100 m dpl.) dari bulan Januari sampai Desember 2009. Penelitian menggunakan rancangan acak kelompok dengan  sembilan perlakuan dan tiga ulangan. Hasil penelitian menunjukkan bahwa  pengasapan dari hasil pembakaran tempurung kelapa yang ditambah dengan Prima BAPF dapat merangsang pertumbuhan tunas pada subang gladiol, tetapi tidak mampu menekan jumlah subang terinfeksi dan intensitas penyakit busuk Fusarium di gudang penyimpanan. Untuk percobaan yang dilakukan di lapangan, perlakuan gabungan antara pengasapan dari hasil pembakaran tempurung kelapa yang ditambah dengan belerang dan Prima BAPF merupakan perlakuan terbaik untuk mengendalikan penyakit layu Fusarium. Aplikasi perlakuan tersebut  menurunkan jumlah tanaman layu, menurunkan nilai AUDPC  perlakuan, dan dapat meningkatkan produksi  bunga gladiol. Hasil penelitian ini diharapkan dapat diadopsi oleh petani guna mengendalikan F. oxysporum f. sp. gladioli secara luas.<br /><br /><br />Gladiolus (Gladiolus hybridus L) is one of the most economically important cut flowers in Indonesia.  The crops is commonly cultivated in the highland. Cultivations of the crops in the production centers have faced various problems especially wilt disease caused by Fusarium oxysporum  f. sp. gladioli  as the most important one. Based on the field observations, it was known that the disease can reduce plant production and its yield quality up to 100%. The experiment was aimed to determine the effect of fumigation by using smoke produced by burned up coconut shell and biopesticide  on gladioulus bud growth and fusarial wilt  incidence. The expereiment was carried out at the Laboratory, Glasshouse and the field of Indonesian Ornamental Crops Research Institute (1,100 m asl.)  since January to December 2009.  A randomized block design with nine treatments and three replications was used.  The results  indicated that fumigation by using smoke of  burned up coconut shell combined with biopesticide Prima BAPF stimulated gladiolus bud growth, but did not suppress infection of the bulb and fusarial disease intensity at the storage.  Base on the field trial, fumigation by smoke of burned up coconut shell combined with sulphur and Prima BAPF was proven to be the best treatment. Application of the treatment significantly reduced disease intensity, AUDPC value, and increased flower production. This research result is expected to be adopted by farmers in order to widely control the F. oxysporum f. sp. gladioli.<br /><br />

Anyelir (Dianthus caryophillus  L.) merupakan salah satu jenis tanaman hias yang mempunyai nilai ekonomi tinggi. Penyakit utama yang menyerang tanaman ini ialah layu Fusarium yang disebabkan oleh Fusarium oxysporum f. sp. dianthi dapat menurunkan kualitas dan kuantitas produksi tanaman sekitar 20-60%.  Pengendalian yang selama ini dilakukan oleh petani bertumpu pada penggunaan  pestisida kimia sintetik. Namun penggunaan bahan kimia tersebut tidak mampu mengeradikasi patogen secara sempurna, terutama pada lapisan tanah yang agak dalam. Salah satu cara pengendalian berwawasan lingkungan ialah menggunakan musuh alami.  Penelitian ini dilaksanakan di Laboratorium dan Rumah Kaca Balai Penelitian Tanaman Hias (1.100 m dpl.), sejak Mei sampai Desember 2009. Penelitian bertujuan mengetahui pengaruh Bacillus subtilis dan Pseudomonas  fluorescens yang diformulasi dalam bentuk biopestisida organik cair dalam pengendalian layu Fusarium pada tanaman anyelir. Rancangan yang digunakan ialah acak  kelompok dengan 10 perlakuan, yaitu 10% ekstrak kascing + 10% molase + B. subtilils + P. fluarescens (BP) dan 10% ekstrak pupuk kandang kuda + 10% molase + BP masing-masing konsentrasi 0,1, 0,3, 0,5, dan 0,7%, dazomet 0,2%, serta kontrol (air ledeng), dengan empat ulangan. Hasil penelitian menunjukkan bahwa populasi bakteri antagonis setelah dilakukan proses fermentasi selama 3 minggu,  meningkat dibandingkan sebelum fermentasi.  Rerata populasi awal sebelum fermentasi bakteri antagonis  107-109 cfu/ml meningkat menjadi 1010-1012 cfu/ml pada 3 minggu setelah fermentasi. Populasi kedua agens biokontrol tersebut setelah penyimpanan selama 2 bulan cenderung stabil berkisar antara 1010-1011 cfu/ml.  Perlakuan B. subtilis dan P. fluorescens  yang disuspensikan ke dalam ekstrak kascing + molase pada taraf konsentrasi 0,5% kemudian difermentasikan dalam biofermentor selama 3 minggu secara konsisten dapat menekan serangan F. oxysporum f. sp. dianthi pada anyelir. Implikasi hasil penelitian ini dapat meningkatkan daya saing komoditas tanaman hias melalui pemanfaatan sumber daya alam nasional secara optimal berkelanjutan untuk mendukung industri tanaman hias yang berdaya saing tinggi.<br /><br />Carnation (Dianthus caryophillus  L.) is one of the most economically important cut flowers in Indonesia. The crops is commonly cultivated in the highland areas of the country. Cultivations of the crops in the production center areas have faced various problems, especially wilt disease caused by Fusarium oxysporum  f. sp. dianthi as the most important one. Based on the field observation it is known that the disease could reduce plant production and its yield quality up to 20-60%. To control the disease, farmers usually use a synthetic chemical pesticides.  However the control measures are not sufficiently effective to overcome the diseases problems.  Therefore, an alternative control measures which are more environmentally friendly is necessary. The use of biocontrol agents is nowdays bring popular to be recommended to  control the disease.  A study on the control of fusarial wilt disease on carnation was carried out  in the Laboratory and Glasshouse of Indonesian Ornamental Crops Research Institute (1,100 m asl.) from May to December 2009, using Bacillus subtilis and Pseudomonas  fluorescens  formulated in the liquid organic pesticide.  The study was arranged in a randomized block design, with 10 treatments i.e. 10% vermi compost + 10% molase + BP and 10% horse manure + 10% molase + BP consentration 0.1, 0.3, 0.5, 0.7% resfectively, dazomet 0,2% and control with four replications. The results showed that population of antagonistic bacterial was increased from  107-109  to 1010-1012 cfu/ml  after 3 weeks fermentation in the organic carrier.  The population of two antagonistic bacteria  was likely stable on 1010-1011 cfu/ml after storing 2 months. The treatments of B. subtilis and P.  fluorescens suspended in the vermi compost extract and molases on the concentration level of  0.5% and formulated in the biofermentor for 3 weeks  were consistenly effective in reducing Fusarium wilt on carnation. The implication of research results could be increase commodity competitive ability of ornamental plants by using national nature resource on a continuity for support the ornamental plants industry with high competitiveness.<br /><br />

<p>Puccinia horiana Henn. merupakan patogen penting penyebab penyakit karat putih yang menimbulkan kerugian signifikan dalam budidaya krisan, baik bunga potong atau tanaman pot. Aplikasi fungisida sintetik yang sering diandalkan oleh petani dan pengusaha tidak hanya memerlukan biaya yang lebih mahal, namun juga berdampak pada kerusakan lingkungan. Oleh karena itu pemanfaatan fungisida nabati yang lebih murah dan ramah terhadap lingkungan dapat menjadi alternatif pemecahannya. Beberapa fungisida nabati seperti Cees EC, Neem-plus, Cekam EC, dan Sitron-E berbahan aktif minyak atsiri cengkih, nimba, kayu manis, serai wangi, dan asam salisilat telah diproduksi dan dikomersialisasikan oleh Balai Penelitian Tanaman Rempah dan Obat. Aplikasi fungisida tersebut diduga berpengaruh positif dalam menekan penyakit karat putih. Penelitian bertujuan untuk memperoleh informasi keefektifan empat produk fungisida nabati tersebut dalam  mengendalikan penyakit karat putih dan meningkatkan kualitas pertumbuhan  krisan. Penelitian dilakukan di Rumah Plastik di Poncokusumo, Malang, Jawa Timur sejak Bulan Januari hingga Desember 2010. Bahan tanaman yang digunakan ialah Dendranthema grandiflora cv. Swarna Kencana. Perlakuan yang diuji ialah 3 ml/l untuk Cees EC, Neem-plus, Cekam EC, dan Sitron-E, serta 1,5 ml/l Amistartop 35 EC sebagai kontrol positif dan air sebagai kontrol negatif. Percobaan disusun menggunakan rancangan acak kelompok dengan enam perlakuan dan empat ulangan. Hasil penelitian menunjukkan bahwa semua fungisida nabati yang diuji efektif mengendalikan penyakit karat pada krisan. Perlakuan tersebut menurut uji statistik memiliki kemampuan yang sebanding dengan Amistartop. Penurunan intensitas karat putih oleh  perlakuan Cees EC, Neem-plus, Cekam EC, Sitron-E, dan Amistartop berturut-turut  sebesar  49; 37,74; 32,43; 29,78; dan 48,33%.  Aplikasi  Cees EC, Neem-plus, Cekam EC, dan Sitron-E tidak berpengaruh nyata terhadap tinggi tanaman, diameter batang, diameter bunga, dan vaselife bunga. Aplikasi hasil penelitian ini dapat memberi manfaat  untuk petani dan pengusaha dalam menurunkan biaya produksi serta meningkatkan pendapatan petani dan pengusaha karena harga keempat biofungisida murah dan tanpa dampak negatif terhadap kelestarian lingkungan.</p><p> </p><p>Controlling white rust disease (Puccinia horiana Henn.) on chrysanthemum with some biofungicides P. horiana Henn. is important pathogen causing white rust disease  that may  lead to a significant lost in chrysanthemum cultivation (both for cut flower and pot plant). Synthetic fungicide commonly applied by farmers are causing not only high production costs, but also endangering the environment.  Confronting to this situation, the use of biofungicide that are considered cheaper and more environmental friendly has become  a relevant and promising alternative. Several biofungicides such as Cees EC, Neem-plus, Cekam EC, and Sitron-E with active ingredient of clove oil, neem, cinnamon, citronella, and salicylic acid have been commersialized by the Indonesian Medical and Spice Crops Research Institute. In this study those  biofungicides were hypothesized to have great potential in control the white rust  disease. The main objective of this study was to obtain information regarding  the efficacy of  four  biofungicides in controlling white rust disease on  chrysanthemum. The  experiment was conducted at Plastichouse in Poncokusumo, Malang, East Java from January to December 2010 by using Dendranthema grandiflora cv. Swarna Kencana as planting materials. The treatments were consisted of 3 ml/l application of Cees EC, Neem-plus, Cekam EC, and Sitron-E 1.5 ml/l application of difenokonazol + azoxistrobin (Amistartop 35 EC) as a positive control  and water as negative control. The experiment was set up  using a randomized block design with six treatments and four replications. The results showed that all tested biofungicides  were quite effective in  controlling white rust disease on chrysanthemum and had similar effectiveness in  reducing  disease intensity compared to  Amistartop. Cees EC, Neem-plus, Cekam EC, Sitron-E, and Amistartop had been able to reduce the white rust disease intensity by 49; 37.74; 32.43; 29.78; and 48.33% respectively. In the meantime, those biofungicides did not show significant effect on plant height, stem diameter, flower diameter, and flower vaselife. The use of biofungicides seems potentially promising to increase farmers income because the price of biofungicides were cheap and maintain environmental sustainability.</p>

<p>Abstrak. Pada beberapa jenis cendawan karat dilaporkan terdapat mikoparasit diantaranya <em>Cladosporium</em> sp. Mikoparasit asal Kebun Percobaan Segunung Balai Penelitian Tanaman Hias (Balithi) yang diduga sebagai <em>Cladosporium</em> menghasilkan penekanan yang tinggi terhadap karat putih asal krisan dengan penekanan mencapai 100%. Penelitian bertujuan  mengidentifikasi dan mempelajari karakter morfologi dan molekuler cendawan mikoparasit yang terdapat pada pustul karat krisan asal kebun percobaan Balithi.   Penelitian dilakukan di Laboratorium Penyakit Balithi Segunung dan Laboratorium Biologi Lembaga Ilmu Pengetahuan Indonesia (LIPI) Cibinong pada Bulan Januari sampai dengan Desember 2012. Identifikasi secara morfologi dilakukan dengan melihat  ciri makroskopis dan mikroskopis cendawan. Identifikasi secara molekuler dilakukan berdasarkan analisis pada sekuen <em>Internal Transcribed Spacer</em> (ITS) <em>ribosomal DNA</em>. Amplifikasi PCR pada ITS menggunakan Primer ITS 4:  5`-- TCC TCC GCT TAT TGA TAT GC – 3` dan ITS 5: 5`--GGA AGT AAA AGT CGT AAC AAG G –3`. Hasil  pengamatan makroskopik  dan mikroskopik menunjukkan bahwa Isolat  567 yang menginfeksi karat putih pada krisan asal Segunung termasuk ke dalam genus <em>Cladosporium. </em>Hasil analisis secara molekuler <em>Cladosporium </em>itu memiliki homologi 100% dengan <em>Cladosporium cladosporioides<strong> </strong></em>strain<strong> </strong>1-09 <strong><em> </em></strong>(aksesi no: JF502459) yang berada di <em>genbank</em>.</p>