Background: Ammoides pusilla plant is a species of therapeutic interest which used in traditional medicines. This work aims to valorize this plant by the characterization of their bioactive components and the evaluation of the in vivo anti-inflammatory potential against a severe disease affecting the bone structure and the stability of the articular cartilage. Methods: First, the phytochemical screening of the polyphenolic extracts of A. pusilla was carried out. The second part of our study is devoted to the evaluation of the in vivo anti-inflammatory activity of the aqueous extract of A. pusilla based on the method of Freund's adjuvant-induced rheumatoid arthritis. Results: Phytochemical tests demonstrated the richness of extract with flavonoids, tannins, coumarins, anthocyanins and triterpenes. Whereas, the quantitative determination reveals that the aqueous extract of A. pusilla is the richest with bioactive components with contents of total polyphenols, flavonoids and tannins equal to 9.52 ± 0.11 mg gallic acid/g, 4.75 ± 0.05 mg quercetin/g and 8.64 ± 0.02 mg catechin/g respectively. The results of the anti-inflammatory activity showed that the aqueous infused extract of A. pusilla has an interesting antiarthritic potential on Freund's adjuvant-induced rheumatoid arthritis in mice. It is manifested by a weight gain; a normal arthritic index and biochemical parameters close to those of Diclofenac®. Conclusion: The aqueous infused extract of A. pusilla is therefore of considerable therapeutic interest as an alternative compound for the prevention of inflammation and for the improvement in bone structure.
Objective / Purpose: Essential oils (EOs) distelled form aromatic plants have a wide range of uses because of their rich pharmacological activities including, antiinflammatory, antitumor, antimicrobial, antioxidant, antidiabetic and hepatoprotective. The present research aimed to investigate in-vivo anti-inflammatory effects of EOs from Rosmarinus officinalis and Populus alba as biomarker levels in well-defined acute and chronic inflammation models. Material and Methods: The anti-inflammatory activity of EOs of R. officinalis and P. alba was carried out using two model, one of them is acute inflammation that induction by dextran 1% at the rat paw 1 and another is chronic inflammation that induction by monoiodoacetic solution.2 To evaluate the anti-inflammatory properties of EOs, a measure of paw thickness was carried out with the calculation of percent inhibition. Thus, monitoring of some biomarkers will be paramount. Results / Discussion: The results indicate the absence of severe clinical signs or dead in rats during the observation period. Therefore, the EOs of R. officinalis and P. alba are devoid of acute toxicity in rats. For treatment with the EOs of R. officinalis, P. alba and Diclofenac®, the results showed a significant reduction for responses induced by dextran. Treatments produced reductions in inflammation ranging from 2.19 to 15.15%. Treatment with EOs showed a recovery of values of biochemical markers during the experimental period. Conclusion: The EOs of R. officinalis and P. alba demonstrate anti-inflammatory effect on both acute and chronic inflammation models, thus they could be used for pharmacological purposes as anti-inflammatory agents.
Abstract-Anacyclus valentinus L. is a common annual plant in
There are many plants which were the subject of recent research in the fields pharmaceutical, cosmetic and agroalimentary because of their chemical composition and their several therapeutic activities. Among these plants, the species of Rosmarinus officinalis (rosemary) and Populus alba (white poplar).The present study aims to examine the yield and chemical properties of essential oil of rosemary and white poplar. Then, the test of skin tolerance of essential oils was conducted by the measuring of primary irritation index. The results of chemical analysis reveal that the essential oils of the both plants are of acceptable quality. In other hand, the essential oil of Rosmarinus officinalis is non-irritating to the skin while the essential oil of Populus albais slightly irritating. This study is essential to ensure the safety of people in contact with substances in pharmaceutical applications. Keywords: Study of Chemical Properties and Skin Tolerance Activity of Essential Oils Extracted from rosmarinus Officinalis and Populusalba 2/4Copyright: ©2017 Hamza et al.was dried with a little an hydroussodium sulfate. Then we make a concentration by rotary evaporator to obtain the essential oil. The essential oil obtained was stored at +4 °C after the calculation of the yield of extraction. Organoleptic charactersAccording to AFNOR NF ISO 280: 1999, essential oils must respond to analytical characteristics that are established by international committees of experts. To know the quality of the EO of R. officinalis (Rosemary) and P. alba (white poplar), standard organoleptic tests like color and odor were performed. a. Chemical indexes: [8] b. pH: This measurement was taken using a pH meter.Acid index: (NF T 75 103, 1982) The acid expresses the number of milligrams of potassium hydroxide (KOH) required to neutralize the free acids contained in one gram of essential oil. 2 g of essential oil was added to 5 ml of ethanol 95% and 5 drops of phenolphthalein at 0.2%. Ester index: (NFT 75 104, 1982) The ester value is the number of milligrams of KOH needed to neutralize the free acids by hydrolysis of esters contained in one gram of essential oil. 2 g of essential oil was added to 25ml of ethanol solution of KOH (0.5 mol / l). It adapts the condenser and placed the ball on the heating mantle and allowed to heat for one hour. Then, the solution was added to 20ml of distilled water and 5 drops of 0.2% PP. The excess of KOH solution was titrated with hydrochloric acid 0.5 mol / l. A blank test was carried out under the same conditions and with the same reagents. The calculation of EI was given by the formula (2) Skin tolerance test (PI):The skin tolerance test aims to predict from the knowledge of the toxicity on a living organism, the toxicological risk in humans. The index of primary skin irritation (PI)of our extracts was determined by official method published in the Official Journal of the French Republicon 21February 1982. The method was based on the observation of skin reactions caused by the applying of principle...
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