Abstract. The chemical composition of honey varies according to the quality of the harvested nectar. The aim of the study was to determine the qualitative and quantitative composition of honey from different regions in Algeria. A qualitative and quantitative analysis was carried out on honey polyphenol extract with the characterization of the majors groups and the determination of the amount of total polyphenols. The results indicate that the honey polyphenol extracts have different rates of phenolic compounds (Flavonoids, Tannins and Cardenolides). The polyphenol extracts present a concentration of 0.32, 0.4, 0.76 and 0.39 mg GAE/100 g for the extract of Mascara, Oran, Jijel and Tlemcen respectively. The different types of tested honey present a variable composition on phenolic compounds, which allowed us to determine the different therapeutic properties of honey.
The adsorption of Cu(2+), Zn(2+), Cd(2+) and Pb(2+) ions from aqueous solution by Algerian raw diatomite was studied. The influences of different sorption parameters such as contact pH solution, contact time and initial metal ions concentration were studied to optimize the reaction conditions. The metals ions adsorption was strictly pH dependent. The maximum adsorption capacities towards Cu(2+), Zn(2+), Cd(2+) and Pb(2+) were 0.319, 0.311, 0.18 and 0.096 mmol g(-1), respectively. The kinetic data were modelled using the pseudo-first-order and pseudo-second-order kinetic equations. Among the kinetic models studied, the pseudo-second-order equation was the best applicable model to describe the sorption process. Equilibrium isotherm data were analysed using the Langmuir and the Freundlich isotherms; the results showed that the adsorption equilibrium was well described by both model isotherms. The negative value of free energy change ΔG indicates feasible and spontaneous adsorption of four metal ions on raw diatomite. According to these results, the high exchange capacities of different metal ions at high and low concentration levels, and given the low cost of the investigated adsorbent in this work, Algerian diatomite was considered to be an excellent adsorbent.
Ficus carica L. have always been known by their power to cure various diseases. This study aims to evaluate the antioxidant activity of twelve different extracts of three samples of dried figs. First, the extracts were tested for their polyphenolic, flavonoid and tannin contents by the Folin–Ciocalteu, the aluminium trichloride and vanillin methods and high-performance liquid chromatography (HPLC) for more characterisation. The antioxidant activity was performed by using thre different methods; the 2,2-diphenyl-2-picrylhydrazyl (DPPH), Ferric reducing antioxidant power (FRAP) and total antioxidant capacity (TAC). The results showed that the methanolic extract of Sidi Bendjebbar sample presented a higher TPC value (458 mg GAE /g) while the acetonic and aqueous extracts of El-Keurt presented a higher values of TFC and CTC. In other hand, the methanol extract of El-Keurt sample exhibited the highest antioxidant capacity with an IC50 of 0.078 mg/ml. The antimicrobial activity of the extracts against various Gram positive and Gram negative bacteria was screened by the inhibition zone using the disc and diffusion assay, minimal inhibition concentration (MIC) by micro-well method allonwing to calculate the fractional inhibitory concentration (FIC). The ethanolic extracts of the two samples El-Keurt and Sidi Bendjebbar were the most effective extracts being able to inhibit the growth of the majority of the strains tested. Our study supported the use of these fruit as supplements for nutrient deficiencies and for combating diseases associated with oxydative damage or some microbial infection for better drug alternatives.
In this study, the phenolic composition, antioxidant and antimicrobial activities of Zizyphus lotus and Ruta chalepensis collected from Mascara-Western Algeria were investigated. The total phenolic, flavonoid and tannin contents in the methanolic (Me.E) and aqueous (Aq.E) extracts were measured using colorimetric methods. Polyphenolic profiles were analyzed by high performance liquid chromatography (HPLC), while the antioxidant effect was determined by DPPH* radical scavenging assays. The antimicrobial activity was estimated using agar disc diffusion and microdilution methods. The results showed that total phenolic compounds, flavonoids and tannins were significantly higher (p <0.05) in Z. lotus extracts when compared with R. chalepensis. The major phenolic compound detected in Z. lotus was benzoic acid (1333.59 µg/g DE), while the hydroxybenzoic acid (56.60 µg/g DE), chlorogenic acid (44.60 µg/g DE), epicatechin (38.80 µg/g DE), catechin (26.30 µg/g DE) and gallic acid (13.22 µg/g DE) were the major compounds in R. chalepensis. Both plants were determined to be effective antioxidants regarding their lower IC50 values of 0.146 mg/mL for Z. lotus Me.E and 0.206 mg/mL for R. chalepensis Aq.E. Results demonstrated that Me.E of Z. lotus and R. chalepensis were more active against S. enterica ssp arizonae, while the lowest minimum inhibitory concentration was recorded against H. alvei using Z. lotus aqueous extract (25 mg/mL). An important anti-Candida activity was also determined. These results suggest the most efficiency of both plants, in the treatment of various human infections, regarding their potential on bioactive molecules with antioxidant and antimicrobial activities.
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