Study of Chemical Properties and Skin Tolerance Activity of Essential Oils Extracted from rosmarinus Officinalis and Populusalba

Abstract

There are many plants which were the subject of recent research in the fields pharmaceutical, cosmetic and agroalimentary because of their chemical composition and their several therapeutic activities. Among these plants, the species of Rosmarinus officinalis (rosemary) and Populus alba (white poplar).The present study aims to examine the yield and chemical properties of essential oil of rosemary and white poplar. Then, the test of skin tolerance of essential oils was conducted by the measuring of primary irritation index. The results of chemical analysis reveal that the essential oils of the both plants are of acceptable quality. In other hand, the essential oil of Rosmarinus officinalis is non-irritating to the skin while the essential oil of Populus albais slightly irritating. This study is essential to ensure the safety of people in contact with substances in pharmaceutical applications. Keywords: Study of Chemical Properties and Skin Tolerance Activity of Essential Oils Extracted from rosmarinus Officinalis and Populusalba 2/4Copyright: ©2017 Hamza et al.was dried with a little an hydroussodium sulfate. Then we make a concentration by rotary evaporator to obtain the essential oil. The essential oil obtained was stored at +4 °C after the calculation of the yield of extraction. Organoleptic charactersAccording to AFNOR NF ISO 280: 1999, essential oils must respond to analytical characteristics that are established by international committees of experts. To know the quality of the EO of R. officinalis (Rosemary) and P. alba (white poplar), standard organoleptic tests like color and odor were performed. a. Chemical indexes: [8] b. pH: This measurement was taken using a pH meter.Acid index: (NF T 75 103, 1982) The acid expresses the number of milligrams of potassium hydroxide (KOH) required to neutralize the free acids contained in one gram of essential oil. 2 g of essential oil was added to 5 ml of ethanol 95% and 5 drops of phenolphthalein at 0.2%. Ester index: (NFT 75 104, 1982) The ester value is the number of milligrams of KOH needed to neutralize the free acids by hydrolysis of esters contained in one gram of essential oil. 2 g of essential oil was added to 25ml of ethanol solution of KOH (0.5 mol / l). It adapts the condenser and placed the ball on the heating mantle and allowed to heat for one hour. Then, the solution was added to 20ml of distilled water and 5 drops of 0.2% PP. The excess of KOH solution was titrated with hydrochloric acid 0.5 mol / l. A blank test was carried out under the same conditions and with the same reagents. The calculation of EI was given by the formula (2) Skin tolerance test (PI):The skin tolerance test aims to predict from the knowledge of the toxicity on a living organism, the toxicological risk in humans. The index of primary skin irritation (PI)of our extracts was determined by official method published in the Official Journal of the French Republicon 21February 1982. The method was based on the observation of skin reactions caused by the applying of principle...

Acute skin irritation, acute and sub-acute oral toxicity studies of Rosmarinus officinalis essential oils in mice and rabbit

“…Low camphor content (3.845%) of R. officinalis essential oil might be responsible for absence of skin irritation since camphor is an irritant substance. This finding is in good agreement with that of Hamza et al (2017), who reported R. officinalis essential oil was non-irritant to the skin though the study was conducted in rat.…”