Radiographic and Histopathologic Analysis on Osteoarthritis Rat Model Treated with Essential Oils of Rosmarinus officinalis and Populus alba

Belkhodja, Hamza; Meddah, Boumédiene; Tirtouil, A.; Slimani, Khaled; Tou, Abdenacer

doi:10.15171/ps.2017.03

Cited by 11 publications

(12 citation statements)

References 9 publications

(5 reference statements)

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“…In line with our results, Belkhodja et al have shown that the rosemary essential oil reduces osteoarthritis histological score in rats with MIA injection [13]. Their results have demonstrated that although rosemary decreases the degradation of articular cartilage and inflammation in the joint, but causes surface fibrillation and deep fissures in cartilage.…”

Section: Discussionsupporting

confidence: 92%

“…Also, Belkhodja et al have studied the effect of rosemary and white poplar essential oils in an animal model of knee osteoarthritis. Radiographic and histologic data of this study have shown that the essential oil has a protective effect against osteoarthritis in rats [13]. Ghasemzadeh and colleagues have evaluated the anti-allodynic and anti-hyperalgesic effects of rosemary extract in rats with neuropathic pain.…”

Section: Introductionmentioning

confidence: 84%

“…Samples were processed in a tissue processor and paraffin-embedded. Five micrometers-thick tissue sections were stained with Hematoxylin and Eosin [13].…”

Section: Histopathological Studymentioning

confidence: 99%

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Anti-osteoarthritis potential of peppermint and rosemary essential oils in a nanoemulsion form: behavioral, biochemical, and histopathological evidence

Mohammadifar

Aarabi

Aghighi

et al. 2021

BMC Complement Med Ther

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Background This study aimed to evaluate the effect of nanoemulsion containing peppermint and rosemary essential oils in rats with osteoarthritis (OA). Methods In this experimental study, we prepared a nanoemulsion containing peppermint and rosemary essential oils by spontaneous emulsification and evaluated the nanoemulsion’s dermal irritation and toxicity. Investigating the analgesic effect of the nanoemulsion, we randomly assigned 36 male rats to 6 groups: Control (saline injection into the knee), osteoarthritis (intra-articular injection of 2 mg monosodium iodoacetate), and four groups of OA treated with nanoemulsion gel, nanoemulsion solution, rosemary and peppermint essential oil gel, or diclofenac sodium. Treatments were administered topically at a dose of 1 ml daily. Using behavioral tests, we assessed pain on days 1, 4, 7, and 14 after injection. Finally, we did the histopathological and biochemical evaluation of rats’ knee joints. Results There were no irritation signs on the animals’ skin after receiving the nanoemulsion and no changes in the hematological and biochemical parameters of rats’ blood compared to the control group. Receiving nanoemulsion decreased the mechanical (P < 0.001) and thermal allodynia (P < 0.05), thermal hyperalgesia (P < 0.05), and ambulatory-evoked pain in comparison with the OA group. Also, the nanoemulsion receiving rats showed an increase in SOD and GPx activity and a decrease in MDA level. Histopathology of synovial tissues confirmed the results of behavioral and biochemical tests. Conclusion The nanoemulsion containing essential oils of peppermint and rosemary reduces osteoarthritis pain via increasing antioxidant capacity and improving the histopathological features of the rats’ knee joint.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 84%

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Anti-osteoarthritis potential of peppermint and rosemary essential oils in a nanoemulsion form: behavioral, biochemical, and histopathological evidence

Mohammadifar

Aarabi

Aghighi

et al. 2021

BMC Complement Med Ther

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“…In this context, Belkhodja et al performed radiographic and histologic evaluations of the effects of Rosmarinus officinalis and Populus alba on Wistar rat models of knee osteoarthritis. Results showed significant decreases in the Mankin scores for EO-treated mice compared to the untreated group, with a slightly higher difference for the Populus alba group [100]. Furthermore, Funk et al investigated the anti-inflammatory activity of ginger EOs in female Lewis rats with streptococcal cell wall-induced arthritis.…”

Section: Repair Phase Key Eventsmentioning

confidence: 99%

Essential Oils for Bone Repair and Regeneration—Mechanisms and Applications

et al. 2021

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Although bone possesses a remarkable capacity for self-remodeling and self-healing of small defects, the continuously increasing growth of bone diseases in the elderly population is becoming a significant burden, affecting individual life quality and society. Conventional treatment options involve surgical procedures for repair and reconstruction, local debridement, autografts or allografts, bone transport, Masquelet’s two-stage reconstructions, and vascularized bone transplants. However, as such approaches often lead to disruptions of bone-regeneration processes and microbial contaminations and are often inefficient, researchers focus on developing bone-regenerative strategies and identifying novel therapeutic agents that could aid the bone-healing process. In this regard, plant-derived biocompounds, especially essential oils (EOs), have received great scientific attention in recent years, owing to their antioxidant, anti-inflammatory, and antimicrobial effects. Current studies focus on either the direct application of EOs on bone tissue or the introduction of EOs as bioactive compounds in bone scaffolds or as coatings for bone implants. Some of the EOs investigated involve St. John’s wort, rosemary, thyme, ylang, white poplar, eucalyptus, lavender, and grape seed. In this context, the present paper aims to provide an overview of the main mechanisms involved in bone repair and regeneration and the potential of EOs to address and enhance these mechanisms.

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“…MIA is a simple, non-invasive, and fast-progressing chemically induced animal model of OA which is mainly used to evaluate pain behavior [5,6]. MIA directly affects the metabolic balance activity of chondrocytes by disrupting glycolysis as a result of inhibiting the activity of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) enzyme, which eventually leads to cartilage degeneration [7]. MIA exhibits biphasic pain behavior; the early phase is the inflammatory phase (2-6 days post-injection) and the pain is the late phase (>14 days post-injection) [8].…”

Section: Introductionmentioning

confidence: 99%

In Vivo Investigation of the Ameliorating Effect of Tempol against MIA-Induced Knee Osteoarthritis in Rats: Involvement of TGF-β1/SMAD3/NOX4 Cue

et al. 2021

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Osteoarthritis (OA) is a complex disease characterized by structural, functional, and metabolic deteriorations of the whole joint and periarticular tissues. In the current study, we aimed to investigate the possible effects of tempol on knee OA induced by the chemical chondrotoxic monosodium iodoacetate (MIA) which closely mimics both the pain and structural changes associated with human OA. Rats were administrated oral tempol (100 mg/kg) one week post-MIA injection (3 mg/50 μL saline) at the right knee joints for 21 consecutive days. Tempol improved motor performance and debilitated the MIA-related radiological and histological alterations. Moreover, it subsided the knee joint swelling. Tempol decreased the cartilage degradation-related biomarkers as matrix metalloproteinase-13, bone alkaline phosphatase (bone ALP), and fibulin-3. The superoxide dismutase mimetic effect of tempol was accompanied by decreased NADPH oxidase 4 (NOX4), inflammatory mediators, nuclear factor-kappa B (NF-κB), over-released transforming growth factor-β1 (TGF-β1). Tempol decreased the expression of chemokine (C-C motif) ligand 2 (CCL2). On the molecular level, tempol reduced the phosphorylated protein levels of p38 mitogen-activated protein kinase (MAPK), and small mother against decapentaplegic 3 homologs (SMAD3). These findings suggest the promising role of tempol in ameliorating MIA-induced knee OA in rats via collateral suppression of the catabolic signaling cascades including TGF-β1/SMAD3/NOX4, and NOX4/p38MAPK/NF-κB and therefore modulation of oxidative stress, catabolic inflammatory cascades, chondrocyte metabolic homeostasis.

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Radiographic and Histopathologic Analysis on Osteoarthritis Rat Model Treated with Essential Oils of Rosmarinus officinalis and Populus alba

Cited by 11 publications

References 9 publications

Anti-osteoarthritis potential of peppermint and rosemary essential oils in a nanoemulsion form: behavioral, biochemical, and histopathological evidence

Anti-osteoarthritis potential of peppermint and rosemary essential oils in a nanoemulsion form: behavioral, biochemical, and histopathological evidence

Essential Oils for Bone Repair and Regeneration—Mechanisms and Applications

In Vivo Investigation of the Ameliorating Effect of Tempol against MIA-Induced Knee Osteoarthritis in Rats: Involvement of TGF-β1/SMAD3/NOX4 Cue

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