BackgroundSalinity is one of the most widespread agricultural problems in arid and semi-arid regions that makes fields unproductive, and soil salinization is a serious problem in the entire world. To determine the effects of salt stress on soybean seedlings, a proteomic technique was used.ResultsSoybean plants were exposed to 0, 20, 40, or 80 mM NaCl for one week. The effect of treatment at 20 mM NaCl on plant growth was not severe, at 80 mM NaCl was lethal, and at 40 mM NaCl was significant but not lethal. Based on these results, proteins were extracted from the leaves, hypocotyls and roots of soybean treated with 40 mM NaCl. Nineteen, 22 and 14 proteins out of 340, 330 and 235 proteins in the leaves, hypocotyls and roots, respectively, were up- and down-regulated by NaCl treatment. In leaves, hypocotyls and roots, metabolism related proteins were mainly down-regulated with NaCl treatment. Glyceraldehyde-3-phosphate dehydrogenase was down-regulated in the leaf/hypocotyls, and fructokinase 2 was down-regulated in the hypocotyls/root with NaCl treatment. Stem 31 kDa glycoprotein precursor was up-regulated in all three organs with NaCl treatment. Glyceraldehyde-3-phosphate dehydrogenase was specifically down-regulated at the RNA and protein levels by salt stress.ConclusionThese results suggest that metabolism related proteins play a role in each organ in the adaptation to saline conditions.

A proteomic approach was used to identify proteins affected by salt in the halophyte C(4) plant Aeluropus lagopoides (Poaceae) in an attempt to understand the mechanism of salt tolerance. Plants were treated with 450 mM NaCl for 10 days, and proteins were then extracted from the shoots and separated by two-dimensional polyacrylamide gel electrophoresis. A total of 1805 protein spots were detected, of which 39 were up-regulated and 44 were down-regulated by treatment with NaCl. Metabolism-related proteins were up-regulated, whereas photosynthesis-related proteins were down-regulated. Dose-dependence studies showed that the up-regulation continued at NaCl concentrations above 450 mM for defense-related proteins alone. Western blot analysis confirmed the down-regulation of RuBisCO LSU and RuBisCO SSU and severe down-regulation of RuBisCO activase. The activity of glyoxalase I increased with increasing NaCl concentration. Metabolome studies indicated up-regulation of amino acids and down-regulation of tricarboxylic acid cycle-related metabolites. These studies suggest that up-regulation of energy formation, amino acid biosynthesis, C(4) photosynthesis, and detoxification are the main strategies for salt tolerance in A. lagopoides.

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This report describes the 17th Chromosome-Centric Human Proteome Project which was held in Tehran, Iran, April 27 and 28, 2017. A brief summary of the symposium's talks including new technical and computational approaches for the identification of novel proteins from non-coding genomic regions, physicochemical and biological causes of missing proteins, and the close interactions between Chromosome- and Biology/Disease-driven Human Proteome Project are presented. A synopsis of decisions made on the prospective programs to maintain collaborative works, share resources and information, and establishment of a newly organized working group, the task force for missing protein analysis are discussed.

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