Changes in the plant proteome resulting from salt stress: Toward the creation of salt-tolerant crops?

Sobhanian, Hamid; Aghaei, Keyvan; Komatsu, Setsuko

doi:10.1016/j.jprot.2011.03.018

Cited by 145 publications

(84 citation statements)

References 73 publications

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“…Although irrigation is often used as a remedy to supplement inadequate rainfall in drought prone areas, over-irrigation of arable lands may increase soil salinity in the long term, thus worsening the situation [4]. Agriculturally important cereals such as rice and maize, like many other food crops, are sensitive to hyperosmotic stresses [5][6][7]; and thus show reduced productivities when cultivated in saline soils. With the increasing soil salinisation that is projected to affect more than 50% of all arable lands by the year 2050 [8], as well as the growing world population, there is increasing need to develop crops that are well adapted to salt stress.…”

Section: Introductionmentioning

confidence: 99%

Identification and profiling of salinity stress-responsive proteins in Sorghum bicolor seedlings

Ngara

Ndimba

Borch-Jensen

et al. 2012

Journal of Proteomics

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Sorghum bicolor, a drought tolerant cereal crop, is not only an important food source in the semi arid/arid regions but also a potential model for studying and gaining a better understanding of the molecular mechanisms of drought and salt stress tolerance in cereals. In this study, seeds of a sweet sorghum variety, MN1618, were planted and grown on solid MS growth medium with or without 100 mM NaCl. Heat shock protein expression immunoblotting assays demonstrated that this salt treatment induced stress within natural physiological parameters for our experimental material. 2D PAGE in combination with MS/MS proteomics techniques were used to separate, visualise and identify salinity stress responsive proteins in young sorghum leaves. Out of 281 Coomassie stainable spots, 118 showed statistically significant responses (p<0.05) to salt stress treatments. Of the 118 spots, 79 were selected for tandem mass spectrometric identification, owing to their good resolution and abundance levels, and of these, 55 were positively identified. Identified proteins were divided into six functional categories including both known and novel/putative stress responsive proteins. Molecular and physiological functions of some of our proteins of interest are currently under investigation via bioinformatic and molecular biology approaches.

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Section: Introductionmentioning

confidence: 99%

Identification and profiling of salinity stress-responsive proteins in Sorghum bicolor seedlings

Ngara

Ndimba

Borch-Jensen

et al. 2012

Journal of Proteomics

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“…Osmotic stress causes many adverse symptoms, which include, among others: plant wilting, impaired growth and development, lower yield or even its absence. Other negative effects of osmotic stress include disorders in such physiological reactions as photosynthesis and formation of the reactive oxygen species (ROS) which lead to oxidation of proteins, amino and nucleic acids, lipid peroxidation, damage and even cell death (Reddy et al 2004;Sobhanian et al 2011). Osmotic stress also causes an ion homeostasis disorder and a reduction of chlorophyll and carotene content (Slama et al 2007;Quados 2010;Sobhanian et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Changes in the root proteome of Triticosecale grains germinating under osmotic stress

2013

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Osmotic stress causes many adverse symptoms in plants, which include, for example, growth limitation and decrease or even absence of yield. Proteomic analyses of plant responses to stressors could lead to the introduction of crops with high resistance to osmotic stress. Such plants would be characterized by high yield even under unfavorable environmental conditions. In this article we describe changes in the protein profiles occurring in response to mild and moderate osmotic stress in triticale roots. Analysis of the protein profiles of these roots showed an increased abundance of 14 and a decreased abundance of 11 proteins under mild osmotic stress conditions while a moderate osmotic stress caused an increased abundance of 18 and a decreased abundance of 33 proteins. Twenty-five proteins, whose quantity altered under stress were identified using MALDI-TOF mass spectrometry. The identified proteins were classified into the categories of proteins associated with: defense mechanisms, metabolism, transcription, cell structure, protein synthesis, transport and signal transduction. The functions of identified proteins were discussed in relation to osmotic stress. Some of the identified proteins may be responsible for the adaptation of plants to adverse conditions.

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“…These reactions are different than those observed during cold or dry periods (Bogeat-Triboulot et al, 2007;Badowiec et al, 2013). An increase in the expression of ATP synthase was observed in wheat (Wang et al, 2008) and in C4 Aeluropus lagopoides (Poaceae) plants (Sobhanian et al, 2010). A small decrease in expression of ATP synthase may indicate that cellular respiration processes may be disturbed, but expression of NAD-dependent dehydrogenase increased rapidly.…”

Section: Discussionmentioning

confidence: 75%

Tetracycline Accumulation in Pea Seedlings and its Effects on Proteome and Enzyme Activities

Margas¹,

Piotrowicz-Cieślak

Ziółkowska³

et al. 2016

IJAB

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To cite this paper: Margas, M., A.I. Piotrowicz-Cieślak, A. Ziółkowska and B. Adomas, 2016. Tetracycline accumulation in pea seedlings and its effects on proteome and enzyme activities. AbstractAmong antibiotics, tetracyclines are the most commonly used and detected in the environment. In this study, the amount of tetracycline taken up from soil by pea seedlings was analyzed, identified its main site of accumulation in plants and determined also changes in the protein profile of pea. The study demonstrates that pea seedlings take up tetracycline from soil and transport the drug via roots to over-ground parts and then accumulate it in the youngest parts, such as upper stem and leaves. After the taken up of drug, the activity of guaiacol peroxidase is modified and changes in the profile of proteins, as determined by two-dimensional gel electrophoresis occur. The majority of proteins (∼40%) visualized possessed molecular weight between 25 and 37 kDa. Only 8% of the proteins had molecular weight lower than 20 kDa, and 2% greater than 75 kDa. The number of spots in the control samples was 194, which is less by 49 than at the concentration of 150 mg kg -1 of soil. Isoflavone reductase was present only in seedlings growing with tetracycline. Tetracycline uptake from soil modify mainly the changes in biochemical processes connected with protein.

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Changes in the plant proteome resulting from salt stress: Toward the creation of salt-tolerant crops?

Cited by 145 publications

References 73 publications

Identification and profiling of salinity stress-responsive proteins in Sorghum bicolor seedlings

Identification and profiling of salinity stress-responsive proteins in Sorghum bicolor seedlings

Changes in the root proteome of Triticosecale grains germinating under osmotic stress

Tetracycline Accumulation in Pea Seedlings and its Effects on Proteome and Enzyme Activities

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