Proteome analysis of soybean leaves, hypocotyls and roots under salt stress

Abstract

BackgroundSalinity is one of the most widespread agricultural problems in arid and semi-arid regions that makes fields unproductive, and soil salinization is a serious problem in the entire world. To determine the effects of salt stress on soybean seedlings, a proteomic technique was used.ResultsSoybean plants were exposed to 0, 20, 40, or 80 mM NaCl for one week. The effect of treatment at 20 mM NaCl on plant growth was not severe, at 80 mM NaCl was lethal, and at 40 mM NaCl was significant but not lethal. Based on these results, proteins were extracted from the leaves, hypocotyls and roots of soybean treated with 40 mM NaCl. Nineteen, 22 and 14 proteins out of 340, 330 and 235 proteins in the leaves, hypocotyls and roots, respectively, were up- and down-regulated by NaCl treatment. In leaves, hypocotyls and roots, metabolism related proteins were mainly down-regulated with NaCl treatment. Glyceraldehyde-3-phosphate dehydrogenase was down-regulated in the leaf/hypocotyls, and fructokinase 2 was down-regulated in the hypocotyls/root with NaCl treatment. Stem 31 kDa glycoprotein precursor was up-regulated in all three organs with NaCl treatment. Glyceraldehyde-3-phosphate dehydrogenase was specifically down-regulated at the RNA and protein levels by salt stress.ConclusionThese results suggest that metabolism related proteins play a role in each organ in the adaptation to saline conditions.

The proteome response of “Hordeum marinum” to long-term salinity stress

“…Upregulation of rubisco activase was reported in stress-tolerant genotypes under various stresses (Caruso et al 2009;Sobhanian et al 2010;Budak et al 2013;De Abreu et al 2014). Moreover, an increase in Rubisco Activase was seen in our last experiment on cultivated barleys during salinity (Fatehi et al 2012a).…”

“…Moreover, an increase in Rubisco Activase was seen in our last experiment on cultivated barleys during salinity (Fatehi et al 2012a). Downregulation of rubisco activase was reported in sensitive plants under stress conditions (Sobhanian et al 2010;Beritognolo et al 2011). The increase in Rubisco Activase may result from their chaperon function that needs more study (Fatehi et al 2012a).…”

“…Ribosomal protein P1 is a component of large ribosomal subunit (60S), which is an important member of the eukaryotic ribosomal translation complex (Rich and Steitz 1987). Several ribosomal proteins are down-regulated in response to abiotic stress, resulting in retardation of growth and productivity in various plants (Sobhanian et al 2010), these downregulations may lead to salt sensitivity in the mentioned plants. Upregulation of ribosomal proteins was observed in high yield and stress-tolerant plants (Ma et al 2007).…”

“…So it might be involved in responses of plants to abiotic stress. Sobhanian et al (2010) reported that FRK was down-regulated in the hypocotyls/root of soybean (salt-susceptible) under NaCl treatment. FRK was down-regulated in response to salt stress in barley cultivars (Witzel et al 2009) whereas upregulation of FRK was observed in our present study on H. marinum (as a salt-tolerant wild barley).…”

Proteomics of Important Food Crops in the Asia Oceania Region: Current Status and Future Perspectives

“…This important legume crop has been adapted to grow in a wide range of climatic conditions. However, the growth, development, and yield of soybean are greatly affected by several abiotic stresses, such as flooding (reviewed by 160,161 ), drought (reviewed by 162 ), salinity (reviewed by 163 ), and heavy metal cadmium (reviewed by 164 ). The information gathered from recent proteomics research (Table 1) has covered all aspects of plant responses including seed germination 165 , the seedling stage [166][167][168] , the later stages of growth [169][170] , and the seed filling stage…”