Salt stress responses of a halophytic grass Aeluropus lagopoides and subsequent recovery

Sobhanian, Hamid; Motamed, Nasrin; Jazii, Ferdous Rastgar; Razavi, Khadija; Niknam, Vahid; Komatsu, Setsuko

doi:10.1134/s1021443710060063

Cited by 12 publications

(11 citation statements)

References 16 publications

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“…Low accumulation of compatible solutes manifests that the plants exposed to 100 mM NaCl do not perceive the stress. No increase in proline content in 150 mM treated plants when compared with control in A. lagopoides ( Sobhanian et al . 2010 b ) also supports the proposed hypothesis.…”

Section: Discussionmentioning

confidence: 99%

“…The high MDA content observed with 300 mM NaCl at 6 days reduced significantly by combined stress of NaCl and KCl, indicating the involvement of K + in reducing the stress. Sobhanian et al . (2010 b ) have reported progressive increase in superoxide dismutase activity and no change in catalase and ascorbate peroxidase levels in A. lagopoides during high salt stress.…”

Section: Discussionmentioning

confidence: 99%

“…1 D). The high Na + content in roots concomitantly increased by increasing the salt concentration may be because of weak control of Na + ions influx ( Sobhanian et al . 2010 b ).…”

Section: Discussionmentioning

confidence: 99%

“…Previous ecological and physiological studies on A. lagopoides suggested that salt stress leads to reduced plant growth, photosynthesis, K + content and soluble protein and increased accumulation of proline and Na + ( Sobhanian et al . 2010 a , b ; Ahmed et al . 2013 ).…”

Section: Introductionmentioning

confidence: 99%

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Ion homeostasis in a salt-secreting halophytic grass

Sanadhya

Agarwal

2015

AoB PLANTS

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Salinity adversely affects plant growth and development, and disturbs intracellular ion homeostasis, resulting in cellular toxicity. Glycophytes tolerate salinity up to 40 mM NaCl, however halophytes grow luxuriantly in salt-marshes where the salt concentration is 200 mM NaCl or more. Aeluropus lagopoides secretes salt crystals from the adaxial and abaxial leaf surface through salt glands. At high salt concentrations the level of different organic and inorganic osmolytes is elevated to prevent toxicity and maintain a sustainable K+/Na+ ratio. Different transporter genes AlHKT2;1, HAK, SOS1, NHX1, and V-ATPase expression coordinate to regulate ion homeostasis in A. lagopoides.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…1 D). The high Na + content in roots concomitantly increased by increasing the salt concentration may be because of weak control of Na + ions influx ( Sobhanian et al . 2010 b ).…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ion homeostasis in a salt-secreting halophytic grass

Sanadhya

Agarwal

2015

AoB PLANTS

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“…The functions of NACs have been extensively studied in glycophytes but limited studies exist in halophytes. The physiological, biochemical and proteomics studies of Aeluropus lagopoides have been carried out to understand its salinity tolerance mechanism ( Gulzar and Khan, 2001 ; Mohsenzadeh et al, 2006 ; Sanadhya et al, 2015a , b ; Sobhanian et al, 2010 ), however, limited attempts have been made toward isolation and characterization of its stress-responsive genes/TFs. The occurrence of NAC proteins remains restricted to land plants, largely to angiosperms but also includes moss ( Physcomitrella patens ), pteridophytes ( Selaginella moellendorffii ) and conifers ( Shen et al, 2009 ).…”

Section: Discussionmentioning

confidence: 99%

AlNAC4 Transcription Factor From Halophyte Aeluropus lagopoides Mitigates Oxidative Stress by Maintaining ROS Homeostasis in Transgenic Tobacco

Khedia

Agarwal

2018

Front. Plant Sci.

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NAC proteins are a large family of plant-specific transcription factors which regulate both ABA-dependent and -independent gene expression. These transcription factors participate in biotic and abiotic stress-response through intricate regulation at transcriptional, post-transcriptional and post-translational levels. In the present study, AlNAC4 transcription factor was isolated from a salt excreting halophyte Aeluropus lagopoides. The AlNAC4 has an open reading frame of 936 bp, encoding a protein of 312 amino acid, with an estimated molecular mass of 34.9 kDa. The AlNAC4 showed close homology to monocot NACs in the phylogenetic tree. In silico analysis revealed that AlNAC4 possess the characteristic A-E subdomains within the NAC domain. The AlNAC4 showed sixteen post-translational phosphorylation sites. The AlNAC4 transcript was significantly upregulated with dehydration and H2O2 treatments, showing its role in osmotic and oxidative stress, respectively. The recombinant protein showed binding to mono as well as tandem repeats of NAC recognition sequence (NACRS) of the erd1 promoter. This is the first report mentioning that overexpression of AlNAC4 improved oxidative stress tolerance in tobacco transgenics. The transgenics maintained ROS homeostasis during H2O2 treatment. The transgenics showed regulation of stress-responsive genes including CAT, SOD, LEA5, PLC3, ERD10B, THT1 and transcription factors like AP2, ZFP during oxidative stress.Key Message: The AlNAC4 transcription factor from recretohalophyte Aeluropus showed regulation with abiotic stresses and binding to NACRS elements of erd1 promoter. The AlNAC4 tobacco transgenics showed improved growth with oxidative stress.

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Salinity response pattern and isolation of catalase gene from halophyte plant Aeluropus littoralis

Modarresi¹,

Nematzadeh²,

Moradian³

2013

Photosynt.

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The role of the antioxidant defense system in salt tolerance of Aeluropus littoralis has not been yet reported; therefore in the present study, the changes of catalase (CAT) activity in this halophyte plant was investigated and CAT gene was isolated. The leaves of treated and control plants were harvested at various times, starting 1 day prior to initiating treatment, then periodically at 72-h intervals for 21 days. The data collected showed that CAT activity increased significantly with time in plants treated with 200, 400, and 600 mM NaCl when compared with the control plants. Maximum enzyme activity was observed between the 6 th and 12 th day at all NaCl concentrations. CAT gene was isolated and cloned via pTZ57R/T cloning vector in Escherichia coli. CAT gene encoded 494 amino acids and had also high homology of 90, 87, 86, and 86% with CAT genes from Zea mays, Oryza sativa, Triticum aestivum, and Hordeum vulgare, respectively.

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Salt stress responses of a halophytic grass Aeluropus lagopoides and subsequent recovery

Cited by 12 publications

References 16 publications

Ion homeostasis in a salt-secreting halophytic grass

Ion homeostasis in a salt-secreting halophytic grass

AlNAC4 Transcription Factor From Halophyte Aeluropus lagopoides Mitigates Oxidative Stress by Maintaining ROS Homeostasis in Transgenic Tobacco

Salinity response pattern and isolation of catalase gene from halophyte plant Aeluropus littoralis

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