In an attempt to find a treatment for diabetes by Placental mesenchymal stem cells, we induced diabetes in albino mice by injected them with the diabetic agent Alloxan intra peritoneal cavity dose 70 mg / kg of body weight for the group 1 and 2, then injection of the diabetic mice with Placental mesenchymal stem cells in the peritoneal cavity for the group 2 then the glucose levels in the blood of these mice were measured by using a GLUCOTREND 2, while group 3 was control injected with normal saline only. Pancreas tissue where tested by studying histopathological effect of Alloxan, the endocrine pancreas histologically showed decreased in the size and number of Langerhans islets with vacuolar degeneration and necrosis of almost all cells in the atrophied islets. Also, there was congestion of blood vessels in the interlobular space and there was distortion of exocrine pancreas. There was vacuolar degeneration and necrosis of the epithelial cells of the acini, also there was atrophy of acini which lead to edema and dilatation of interacini space. Moreover, there was thickening in the wall of blood vessels, and there was thrombus in some blood vessels. In addition to the infiltration of inflammatory cells in the interlobular septa. Compared with the section of pancreas of control group showed the normal structure of islets notice the normal cells shape surrounding by exocrine pancreas. Then the pancreas studied after injection of Placental mesenchymal stem cells, while there was regeneration of islets and acini which seem to be normal comparing with control group, there was improvement of normal histological appearance of mice treated with alloxan and then injected with placental mesenchymal stem cells. The results of the diabetic mice which treated with placental mesenchymal stem cells demonstrated present of significant differences (P≤0.05). Our conclusion of this study revealed that placental mesenchymal stem cells have a protective role against the histological changes of pancreas induced by alloxan.
The present study includes the investigation of the effect of evaporation with Peganum harmala seeds on hormone FSH, LH, testosterone concentrations and sperm count in male albino rats Rattus norvegicus treated with Chlorpromazine. The rats were aged (3-4) months and weighing (200-300) grams. The first group was a negative control of 10 rats which gavaged with distilled water and the 50 remaining rats gavaged with Chlorpromazine (2 mg / kg body weight) daily for six weeks. The treated animals were divided into 4 groups, each group consists of 10 rats. The positive control was treated with drug only, the second group was evaporated daily for a period of 7 days, the third was evaporated daily for a period of 14 days, the fourth group was evaporated daily for a period of 21 days and the fifth group released for 30 days without treatment. The results showed a significant decrease in LH, FSH, testosterone hormones concentration and sperm count in a group treated with drug chlorpromazine (control group positive) compared to the negative control group. Also the results demonstrate that evaporation rats with Peganum harmala seeds for 7, 14 and 21 days lead to a significant increase in LH, FSH, testosterone hormones concentration and sperm count as compared with positive groups, while hormones concentration and sperm count did not recover to the normal level in the group which was released for 30 days without treatment. From this research, it is concluded that evaporation with Peganum harmala seeds causes rearranging the sexual activity in the rats treated with Chlorpromazine.
In this study, 192 healthy pregnant rats weighting about 200-250 grams were used. The animals were housed under standard laboratory condition (12h dark and 22±2 C°). The animals were given standard rat pellets and tap water ad libtium. Normal female rats mated with normal males. The first day of gestation was detected through vaginal smear for sperms of vaginal plug. Diabetes mellitus was induced by a single subcutaneous injection of 100 mg/kg of alloxan. After four days, rats with blood glucose more than 200 mg/dl were considered as alloxan induced diabetic pregnant, and they were distributed to three periods (1-7, 8-14 and 15-22) days of gestation. Diabetic pregnant rats at each period were divided to eight groups. First group supplemented with standard diet which respect negative control, while the rest groups were treated with 100mg/kg alloxan subcutaneously. In addition to alloxan, third group was treated with 250mg/kg folate and 4 I.U. insulin subcutaneously, group four was daily injected with 7.5mg/kg folate and 4 I.U. insulin subcutaneously, group five was supplemented with 250mg/kg folate, group six was daily injected with 4 I.U. insulin subcutaneously, group seven was supplemented with 0.1% N-acetyl cysteine and 4 I.U. insulin and the rats of the last group were supplemented with 0.1% N-acetyl cysteine. Ovarian sections were prepared during each week of gestation. Histological examination of ovarian sections revealed less multiple follicles in all treatment groups as compared with positive control. Hemorrhage was seen in folic acid (0.25 mg/kg) and insulin and with n-acetyl cysteine and insulin treated groups. Infiltration of eosinophils was seen in folic acid (250 mg/kg) and insulin groups. From these results it was concluded that the histological sections showed decreasing ovarian follicle number in all treatment groups as compared with positive control.
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