SUMMARYThe mud crab (Scylla paramamosain) is a carnivorous portunid crab, mainly distributed along the southeastern coast of China. Mitochondrial DNA analysis in a previous study indicated a high level of genetic diversity and a low level of genetic differentiation. In this study, population genetic diversity and differentiation of S. paramamosain were investigated using nine microsatellite markers. In total, 397 wild specimens from 11 locations on the southeastern coast of China were sampled and genotyped. A high level of genetic diversity was observed, with the number of alleles, and the observed and expected heterozygosity per location in the range 7.8-
Nineteen microsatellite DNA markers of Collichthys lucidus were developed. The number of alleles for each locus ranged from three to 11. A total of 104 alleles were identified in 30 individuals collected from Zhejiang, China. The observed and expected heterozygosity per locus ranged from 0.2857 to 1.000 and from 0.5539 to 0.8774, respectively. Significant deviation from HardyWeinberg equilibrium at four microsatellite loci was detected after Bonferroni correction (P \ 0.0026). These novel loci will be helpful for understanding the background of C. lucidus at genetic level.
The mud crab Scylla paramamosain plays a significant role in fishery resources in China. In this study, we developed 18 polymorphic microsatellite markers in this important crab by 5' anchored PCR technique. A total of 125 alleles were detected in a single population of 32 individuals of S. paramamosain. The number of alleles per locus ranged from five to nine, with the allele size ranging from 166 to 316 bp. The polymorphism information content (PIC), observed and expected heterozygosity ranged from 0.39 to 0.88, from 0.33 to 0.92 and from 0.42 to 0.86, respectively. Three loci (Scypa13, Scypa14 and Scypa15) deviated significantly from Hardy-Weinberg equilibrium (HWE) after Bonferroni correction (P < 0.0028), and no linkage disequilibrium was found between loci pairs. These polymorphic microsatellite markers will be useful for the study of population genetic structure, construction of genetic linkage maps and mapping of economically quantitative trait loci (QTL) in S. paramamosain.
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