Novel Polymorphic Microsatellite Markers in Scylla paramamosain and Cross-Species Amplification in Related Crab Species

Ma, Hongyu; Ma, Chunyan; Ma, Lan; Cui, Haiyu

doi:10.1651/09-3263.1

Cited by 27 publications

(15 citation statements)

References 21 publications

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“…In the present study, a total of five type I microsatellite loci were found to be polymorphic using a G 1 family. The genetic diversity (mean H O = 0.50) was much lower than that of type II loci (mean H O = 0.76; Ma et al, 2010), and even lower than that of type I loci developed in a previous study (mean H O = 0.68; Ma et al, 2014a). Aside from the major reason of microsatellite loci themselves, this difference may be due to the types and sizes of populations (wild, cultured, or a single family) used.…”

Section: Discussionmentioning

confidence: 79%

First genetic linkage map for the mud crab (Scylla paramamosain) constructed using microsatellite and AFLP markers

Ma¹,

Li²,

Feng³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. The mud crab (Scylla paramamosain) is of economic importance for the fisheries and aquaculture industry in China. In this study, we constructed the first genetic linkage map for this species using microsatellite and amplified fragment length polymorphism (AFLP) markers. The map consisted of 65 linkage groups, including 34 triplets and 9 doublets. A total of 212 molecular markers were mapped, including 60 microsatellites and 152 AFLP markers. The linkage groups ranged from 7 to 102.5 cM and covered 2746.4 cM in length. The mean length was 42.3 cM per linkage group, and the mean spacing was 18.68 cM. The genome size was estimated to be 5539.62 cM, with 50% coverage by the present map. Moreover, we reported 5 transcriptomederived polymorphic microsatellite markers and characterized their polymorphism in a first-generation family. This study will facilitate studies on high-density maps and molecular marker-assisted selection in S. paramamosain and related crustacean species.

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Section: Discussionmentioning

confidence: 79%

First genetic linkage map for the mud crab (Scylla paramamosain) constructed using microsatellite and AFLP markers

Ma¹,

Li²,

Feng³

et al. 2016

Genet. Mol. Res.

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“…Then, 148 positive clones were identified and sequenced using an ABI Prism 3730 automated DNA sequencer. In comparison to other species, the positive clone rate in this study was lower than that in fish (Cynoglossus semilaevis) (Liu et al, 2008) and crab (Scylla paramamosain) (Ma et al, 2010). Moreover, 75 pairs of primers were designed by using Primer Premier 5.0 and synthesized.…”

Section: Resultsmentioning

confidence: 89%

Research Article Isolation and characterization of polymorphic microsatellite markers for the chub mackerel (Scomber japonicus) and cross-species amplification in the blue mackerel (S. australasicus).

Chen¹,

Li³

et al. 2017

Genet. Mol. Res.

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ABSTRACT. In this study, 10 polymorphic microsatellite markers were developed in Scomber japonicus and were examined on 30 individuals collected from the North Pacific. The number of alleles per locus ranged from 4 to 17. The observed and expected heterozygosities per locus ranged from 0.2759 to 0.8621 and from 0.43071 to 0.9177, respectively. The polymorphism information content (PIC) was from 0.3931 to 0.8939. One locus showed moderate polymorphism (0.25 < PIC < 0.5), while the rest were highly polymorphic (PIC > 0.5). Two loci showed significant deviation from Hardy-Weinberg equilibrium after Bonferroni corrections (P < 0.005). No linkage disequilibrium was detected among the loci. Results of cross-species amplification showed that 10 microsatellite markers were successfully amplified in 29 individuals of S. australasicus and 9 indicated polymorphisms. These markers will be useful for investigating the genetic structure, gene

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“…Nine polymorphic microsatellite loci were selected for genotyping, of which eight were developed using the 5Ј anchored PCR method (Cui et al, 2011), and the remaining one was developed using PCRbased isolation of microsatellite arrays (PIMA) (Ma et al, 2010) in our laboratory (Table2). The criteria for selection were as follows: annealing temperature of 50-63°C, expected product size between 110 and 320bp, observed heterozygosity value >0.5 and no stuttering bands.…”

Section: Microsatellite Genotypingmentioning

confidence: 99%

“…Microsatellite markers have been widely used for investigation of genetic diversity (Dudaniec et al, 2010), determination of pedigree (Li et al, 2009a), construction of genetic maps (Ma et al, 2011b) and mapping of quantitative trait loci . To date, microsatellite markers have been isolated in S. paramamosain (Takano et al, 2005;Ma et al, 2010;Ma et al, 2011c;Cui et al, 2011), but no information about population genetic diversity and differentiation has been reported for this important crab species.…”

Section: Introductionmentioning

confidence: 99%

High genetic diversity and low differentiation in mud crab (Scylla paramamosain) along southeastern coast of China revealed by microsatellite markers

Cui

et al. 2012

Journal of Experimental Biology

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SUMMARYThe mud crab (Scylla paramamosain) is a carnivorous portunid crab, mainly distributed along the southeastern coast of China. Mitochondrial DNA analysis in a previous study indicated a high level of genetic diversity and a low level of genetic differentiation. In this study, population genetic diversity and differentiation of S. paramamosain were investigated using nine microsatellite markers. In total, 397 wild specimens from 11 locations on the southeastern coast of China were sampled and genotyped. A high level of genetic diversity was observed, with the number of alleles, and the observed and expected heterozygosity per location in the range 7.8-

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Novel Polymorphic Microsatellite Markers in Scylla paramamosain and Cross-Species Amplification in Related Crab Species

Cited by 27 publications

References 21 publications

First genetic linkage map for the mud crab (Scylla paramamosain) constructed using microsatellite and AFLP markers

First genetic linkage map for the mud crab (Scylla paramamosain) constructed using microsatellite and AFLP markers

Research Article Isolation and characterization of polymorphic microsatellite markers for the chub mackerel (Scomber japonicus) and cross-species amplification in the blue mackerel (S. australasicus).

High genetic diversity and low differentiation in mud crab (Scylla paramamosain) along southeastern coast of China revealed by microsatellite markers

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