High genetic diversity and low differentiation in mud crab (<i>Scylla paramamosain</i>) along southeastern coast of China revealed by microsatellite markers

Ma, Hongyu; Cui, Haiyu; Ma, Chunyan; Ma, Lan

doi:10.1242/jeb.071654

Cited by 19 publications

(12 citation statements)

References 29 publications

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“…A microsatellite-based parentage assignment technique was successfully developed for S. paramamosain , and its success rate for assigning progeny to real parents reaches 95% [5]. Several types of genetic markers, including microsatellites [6]–[9], SNP [10], complete mitochondrial DNA [11], and AFLP [12] have been developed to assist in the improvement and enhancement of the economically important traits of S. paramamosain , and the genetic diversity and structure of wild and cultured populations have also been investigated [13], [14]. However, information on the application of molecular technology in the assisted breeding of S. paramamosain is limited.…”

Section: Introductionmentioning

confidence: 99%

Identification of Transcriptome-Derived Microsatellite Markers and Their Association with the Growth Performance of the Mud Crab (Scylla paramamosain)

Jiang

Liu

et al. 2014

PLoS ONE

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Microsatellite markers from a transcriptome sequence library were initially isolated, and their genetic variation was characterized in a wild population of the mud crab (Scylla paramamosain). We then tested the association between these microsatellite markers and the growth performance of S. paramamosain. A total of 129 polymorphic microsatellite markers were identified, with an observed heterozygosity ranging from 0.19 to 1.00 per locus, an expected heterozygosity ranging from 0.23 to 0.96 per locus, and a polymorphism information content (PIC) ranging from 0.21 to 0.95 per locus. Of these microsatellite markers, 30 showed polymorphism in 96 full-sib individuals of a first generation family. Statistical analysis indicated that three microsatellite markers were significantly associated with 12 growth traits of S. paramamosain. Of these three markers, locus Scpa36 was significantly associated with eight growth traits, namely, carapace length, abdomen width (AW), body height (BH), fixed finger length of the claw, fixed finger width of the claw, fixed finger height of the claw, meropodite length of pereiopod 2, and meropodite length of pereiopod 3 (MLP3) (P<0.05). Locus Scpa75 was significantly associated with five growth traits, namely, internal carapace width, AW, carapace width at spine 8, distance between lateral spine 2 (DLS2), and MLP3 (P<0.05). Locus Spm30 was significantly associated with BH, DLS2, and body weight (P<0.05). Further analysis suggested a set of genotypes (BC at Scpa36, BC and BD at Scpa75, and AC at Spm30) that have great potential in the selection of S. paramamosain for growth traits. These findings will facilitate the development of population conservation genetics and molecular marker-assisted selective breeding of S. paramamosain and other closely related species.

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Section: Introductionmentioning

confidence: 99%

Identification of Transcriptome-Derived Microsatellite Markers and Their Association with the Growth Performance of the Mud Crab (Scylla paramamosain)

Jiang

Liu

et al. 2014

PLoS ONE

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“…Many studies have been conducted on the phenotypic and genetic relationships of growth traits in aquaculture animals, such as pearl oyster (Pinctada martensii) (Deng et al, 2008), common carp (Cyprinus carpio) (Wang, 2009), small abalone (Haliotis diversicolor) (You et al, 2010), Japanese flounder (Paralichthys olivaceus) (Tian et al, 2011), and sea urchin (Strongylocentrotus intermedius) (Chang et al, 2012). Research on S. paramamosain has mainly focused on reproductive performance (Djunaidah et al, 2003;Zeng, 2007), culture biology Ut et al, 2007), functional genes (Rosa et al, 2008;Wang et al, 2012), population genetic diversity (Shu et al, 2011;Ma et al, 2011aMa et al, , 2012, and molecular markers (Ma et al, 2011b,c). In addition, limited differences of morphological traits of individuals from different coasts of southeastern China were determined by morphological discriminant analysis (Li et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Characteristics of growth traits and their effects on body weight of G1 individuals in the mud crab (Scylla paramamosain)

Jiang

et al. 2014

Genet. Mol. Res.

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ABSTRACT. The mud crab (Scylla paramamosain) is considered a potentially important marine crab species for selective breeding. Here, we first examined sex ratio and differences in 16 growth traits between females and males in a G 1 population of S. paramamosain, and we then analyzed the correlation between these growth traits and their effects on body weight (BW). Of these growth traits, nine were significantly different between sexes. In females, the correlation coefficients in all trait pairs ranged from 0.524 to 0.997. The traits carapace length (CL) and distance between lateral spine 2 (DLS2) significantly affected BW directly, with the path coefficients being 1.124 and -0.186, respectively. The determination coefficients of traits CL and DLS2 to BW were 1.263 and 0.035 with the total value being 0.951, indicating that the two traits were the key factors affecting BW. In males, the correlation coefficients in all trait pairs ranged from 0.881 to 0.999. The three traits body Characteristics of growth traits of G 1 specimens in mud crab height (BH), fixed finger height of the claw (FFHC), and meropodite length of pereopod 2 (MLP2) significantly affected BW directly, with the path coefficients being 0.484, 0.300, and 0.225, respectively. The determination coefficients of traits BH, FFHC and MLP2 to BW were 0.234, 0.090 and 0.051, with the total value being 0.967, indicating that these three traits played a key role in affecting BW. Moreover, we constructed two best-fit linear regression equations, which were Y (BW) = 4.969 X 1 (CL) -0.758 X 2 (DLS2) -140.177 and Y (BW) = 3.806 X 1 (BH) + 2.371 X 2 (FFHC) + 1.725 X 3 (MLP2) -123.559 in females and males, respectively.

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“…For the year 2011, the total culture area of S. paramamosain had expanded to more than 270 x 10 6 m 2 , and the output reached more than 110,000 tons in China. In the past decade, scientific studies on S. paramamosain have mainly focused on its reproductive biology (Djunaidah et al, 2003;Zeng, 2007), culture biology Ut et al, 2007), functional genes (Rosa et al, 2008;Wang et al, 2012), population genetic diversity (Ma et al, 2011a(Ma et al, , 2012Shu et al, 2011), and molecular markers (Xu et al, 2009;Ma et al, 2011b,c). However, little information about traditional selective breeding or molecular marker-based selection is available for S. paramamosain, although the genetic differences among different F 1 families have been investigated by microsatellite markers (Cui et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Correlation of growth-related traits and their effects on body weight of the mud crab (Scylla paramamosain)

Ma¹,

Ma²,

Ma³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. In this study, we analyzed the correlation of 20 growthrelated traits and their effects on body weight of Scylla paramamosain. The correlation coefficients in all trait pairs were significantly high, ranging from 0.551 to 0.999. Among 19 X-Y pairs, the correlation coefficient between traits X 1 and Y was the highest, whereas that between X 13 and Y was the lowest. Path analysis indicated that only two traits (X 1 and X 14 ) can significantly affect body weight (Y) directly, with the path coefficients being 0.800 and 0.198, respectively. The determination coefficients (d i ) of traits X 1 and X 14 to body weight were 0.640 and 0.039, respectively, and the total d i was 0.965, indicating that both traits were the key factors affecting body weight. Moreover, traits X 1 and X 14 were confirmed to be significantly related to body weight. Finally, a best-fit linear regression equation was constructed as Y = 4.192X 1 + 2.242X 14 -169.737.

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High genetic diversity and low differentiation in mud crab (Scylla paramamosain) along southeastern coast of China revealed by microsatellite markers

Cited by 19 publications

References 29 publications

Identification of Transcriptome-Derived Microsatellite Markers and Their Association with the Growth Performance of the Mud Crab (Scylla paramamosain)

Identification of Transcriptome-Derived Microsatellite Markers and Their Association with the Growth Performance of the Mud Crab (Scylla paramamosain)

Characteristics of growth traits and their effects on body weight of G1 individuals in the mud crab (Scylla paramamosain)

Correlation of growth-related traits and their effects on body weight of the mud crab (Scylla paramamosain)

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