Development and characterization of 19 microsatellite markers for Collichthys lucidus

Abstract

Nineteen microsatellite DNA markers of Collichthys lucidus were developed. The number of alleles for each locus ranged from three to 11. A total of 104 alleles were identified in 30 individuals collected from Zhejiang, China. The observed and expected heterozygosity per locus ranged from 0.2857 to 1.000 and from 0.5539 to 0.8774, respectively. Significant deviation from HardyWeinberg equilibrium at four microsatellite loci was detected after Bonferroni correction (P \ 0.0026). These novel loci will be helpful for understanding the background of C. lucidus at genetic level.

Chromosome assembly of Collichthys lucidus, a fish of Sciaenidae with a multiple sex chromosome system

“…C . lucidus is a commercially important marine fish species with high market value and has been widely consumed in coastal regions in China 2 .

Fig. 1A picture of Collichthys lucidus used for the genome sequencing.

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Fine‐scale population structure of Collichtys lucidus populations inferred from microsatellite markers

“…PCR was carried out in a 25‐μL reaction mixture containing 20 ng template DNA, 0.15 µl (5 U/µL) Taq DNA polymerase (Takara Co., Dalian, China), 1 µl (10 pmol/µL) of each forward and reverse primer and 2 µl (200 µmol/L) of each deoxy‐ribonucleoside triphosphate. PCR amplification was performed in a thermal cycler (Biometra, Germany) under optimized reaction conditions (Lin et al, ; Ma et al, ; Sun et al, ). PCR products were separated using 8% non‐denaturing vertical polyacrylamide gel electrophoresis and visualized by silver staining (Bassam, Caetano‐Anolles, & Gresshoff, ).…”

“…Genomic DNA was isolated from muscle tissue by proteinase K digestion followed by a standard phenol‐chloroform method. A total of 36 microsatellite markers from the previous studies were tested (Lin et al, ; Ma et al, ; Sun et al, ), ten of which were polymorphic and used for this study (Table ). Polymerase chain reaction (PCR) was conducted to amplify the ten polymorphic microsatellite markers.…”

“…PCR amplification was performed in a thermal cycler (Biometra, Germany) under optimized reaction conditions (Lin et al, 2011;Ma et al, 2011;Sun et al, 2011). PCR products were separated using 8% non-denaturing vertical polyacrylamide gel electrophoresis and visualized by silver staining (Bassam, Caetano-Anolles, & Gresshoff, 1991).…”

“…In recent years, microsatellites or simple sequence repeats have been widely applied for studies of population genetics of marine fishes due to theirco-dominant expression, and high polymorphism (Hoshino, Bravo, Macedo, & Morelli, 2012;Zane, Bargelloni, & Patarnello, 2002). Some microsatellite loci for C. lucidus have been isolated and developed (Lin, Su, Ding, & Wang, 2011;Ma, Ma, Ma, Cui, & MA, 2011;Sun, Zhang, Xu, & Wang, 2011). Six microsatellite loci have been employed to analyze genetic diversity of C. lucidus from Zhoushan offshore waters, and low genetic diversity was detected by Lin et al (2011).…”