Meta-analysis techniques were used to examine the effect of elevated atmospheric carbon dioxide [CO 2 ] on the protein concentrations of major food crops, incorporating 228 experimental observations on barley, rice, wheat, soybean and potato. Each crop had lower protein concentrations when grown at elevated (540-958 lmol mol À1 ) compared with ambient (315-400 lmol mol À1 ) CO 2 . For wheat, barley and rice, the reduction in grain protein concentration was $ 10-15% of the value at ambient CO 2 . For potato, the reduction in tuber protein concentration was 14%. For soybean, there was a much smaller, although statistically significant reduction of protein concentration of 1.4%. The magnitude of the CO 2 effect on wheat grains was smaller under high soil N conditions than under low soil N. Protein concentrations in potato tubers were reduced more for plants grown at high than at low concentrations of ozone. For soybean, the ozone effect was the reverse, as elevated CO 2 increased the protein concentration of soybean grown at high ozone concentrations. The magnitude of the CO 2 effect also varied depending on experimental methodology. For both wheat and soybean, studies performed in opentop chambers produced a larger CO 2 effect than those performed using other types of experimental facilities. There was also indication of a possible pot artifact as, for both wheat and soybean, studies performed in open-top chambers showed a significantly greater CO 2 effect when plants were rooted in pots rather than in the ground. Studies on wheat also showed a greater CO 2 effect when protein concentration was measured in whole grains rather than flour. While the magnitude of the effect of elevated CO 2 varied depending on the experimental procedures, a reduction in protein concentration was consistently found for most crops. These findings suggest that the increasing CO 2 concentrations of the 21st century are likely to decrease the protein concentration of many human plant foods.

Summary• Availability of growth limiting resources may alter root dynamics in forest ecosystems, possibly affecting the land-atmosphere exchange of carbon. This was evaluated for a commercially important southern timber species by installing a factorial experiment of fertilization and irrigation treatments in an 8-yr-old loblolly pine ( Pinus taeda ) plantation.• After 3 yr of growth, production and turnover of fine, coarse and mycorrhizal root length was observed using minirhizotrons, and compared with stem growth and foliage development.• Fertilization increased net production of fine roots and mycorrhizal roots, but did not affect coarse roots. Fine roots had average lifespans of 166 d, coarse roots 294 d and mycorrhizal roots 507 d. Foliage growth rate peaked in late spring and declined over the remainder of the growing season, whereas fine roots experienced multiple growth flushes in the spring, summer and fall.• We conclude that increased nutrient availability might increase carbon input to soils through enhanced fine root turnover. However, this will depend on the extent to which mycorrhizal root formation is affected, as these mycorrhizal roots have much longer average lifespans than fine and coarse roots.

No abstract

The growth of many pine plantations in the southern United States is limited by soil nutrient availability. Therefore, forest fertilization is a common silvicultural practice throughout the South. Approximately 1.2 million ac of pine plantations were fertilized in 2004. In the last 10 years, considerable advances have been made in identifying the ecophysiological basis for stand growth and the response to fertilizer additions. Nitrogen (N) and phosphorus (P) are the nutrients that most commonly limit growth of southern pine. On wet clay soils in the lower Coastal Plain and on some well-drained soil in the upper Coastal Plain, severe P deficiencies exist. On these soils, P fertilization with 25–50 lb of P per acre at the time of planting produces a large and sustained growth response, on the order of 50 ft3 ac−1 yr−1 (1.5 tn ac−1 yr−1) throughout the rotation. On most other soils in the South, chronic deficiencies of both N and P exist. On these sites, soil nutrient availability often is adequate early in the rotation when tree demand is small. However, around the time of crown closure, N and P frequently become limiting. Fertilization with both N and P in these intermediate aged stands typically increases growth for 8–10 years. The growth response to a combination of 25 lb of P per acre plus 200 lb of N per acre averages around 55 ft3 ac−1 yr−1 (1.6 tn ac−1 yr−1) for an 8-year period. The amount of leaf area in the stand is the main factor determining the current growth rate of the stand and the potential growth response after fertilization. When stand leaf area index is less than 3.5, light capture by the stand is restricted and growth is negatively affected. In many of these stands, fertilization will increase leaf area because of increased soil nutrient availability and thus increase growth. The financial return after fertilization depends on the growth response that occurs, the cost of the fertilizer treatment, and the stumpage value of the timber produced. Using a growth response of 55 ft3 ac−1 yr−1 over 8 years, a fertilizer cost of $90 ac−1, and stumpage values from the first quarter of 2006, the internal rate of return from midrotation fertilization of a loblolly pine plantation with N and P would be approximately 16%.

Although low solubility and slow cycling control P circulation in a wide range of ecosystems, most studies that evaluate bioavailability of soil P use only indices of short-term supply. The objective here is to quantify changes in P fractions in an Ultisol during the growth of an old-field pine forest from 1957 to 2005, specifically changes with organic P (Po) and with inorganic P (Pi) associated with Fe and Al oxides as well as Ca compounds. Changes in soil P were estimated from archived mineral soil samples collected in 1962 shortly after pine seedlings were planted, and on six subsequent occasions (1968, 1977, 1982, 1990, 1997, and 2005) from eight permanent plots and four mineral soil layers (0-7.5, 7.5-15, 15-35, and 35-60 cm). Despite the net transfer of 82.5 kg ha(-1) of P from mineral soil into tree biomass and O horizons, labile soil P was not diminished, as indexed by anion exchange resins, and NaHCO(3) and Mehlich III extractants. An absence of depletion in most labile P fractions masks major restructuring of soil P chemistry driven by ecosystem development. During 28 years of forest growth, decreases were significant and substantial in slowly cycling Po and Pi associated with Fe and Al oxides and Ca compounds, and these accounted for most of the P supplied to biomass and O horizons, and for buffering labile soil fractions as well. Changes in soil P are attributed to the P sink strength of the aggrading forest (at 2.9 kg ha(-1) year(-1) over 28 years); legacies of fertilization, which enriched slowly cycling fractions of Po and Pi; and the changing biogeochemistry of the soil itself.

No abstract

The ability of soil to sustain its supply of nutrients to a growing forest is controlled by a complex of biogeochemical processes. Forest soil data are notably absent, however, that describe sustained nutrient supply of nutrient depletion. The objective of this study was to evaluate how exchangeable nutrient cations of a previously cultivated Ultisol responded to the first three decades of pine forest development. On six occasions during the three decades, the upper 0.6 m of soil was sampled from eight permanent plots and chemically analyzed with the same procedures. During this period, KCl—exchangeable acidity (as positive charges of adsorbed H and Al ions) increased by 37.3 kmolc/ha in the upper 0.6 m of soil and positive charges of exchangeable Ca and Mg were depleted by 34.8 and 8.9 kmolc/ha (by 696 and 108 kg/ha), whereas, exchangeable K was reduced by only 0.5 kmolc/ha (19 kg/ha). Depletion of soil exchangeable Ca was on the same order of magnitude as Ca removals (i.e., Ca accumulation in biomass and forest floor plus that lost in soil leaching). Removals of soil Mg also appeared to outpace resupply from recycling, atmospheric deposition, and mineral weathering, but not the same degree as Ca. Over the three decades, soil leaching loss of these divalent cations (from 0.6 m depth) appeared equal to cation accumulation in biomass plus forest floor, with sulfate balancing about half these cations in leachates. In contrast to Ca and Mg, total K removals from the soil exceeded reductions in soil exchangeable K by nearly 20—fold. Exchangeable K was well buffered in surface mineral soils apparently due to a combination of biological recycling via leaching of canopies and forest floor plus mineral weathering release. These nutrient dynamics may be common to many nutrient—demanding forest ecosystems supported by soils with low activity kandic or oxic horizons. Such soils (Ultisols and Oxisols) occur on many hundreds of millions of hectares in temperate and tropical zones.

Changing environmental conditions have the potential to alter allometric relationships between plant parts, possibly leading to ecosystem-level feedbacks. We quantified allometric shifts in field-grown loblolly pine (Pinus taeda L.) in response to altered resource availability based on data from multiple harvests to correct for size-related changes in biomass partitioning. A replicated factorial arrangement of irrigation and fertilization treatments was applied for 4 years to an 8-year-old loblolly pine plantation on a well-drained, low fertility site in North Carolina. Destructive and nondestructive growth measurements were used to develop treatment-specific regressions to estimate stand-level biomass for ephemeral and perennial plant parts, both above- and belowground. Stand-level allometric analysis indicated that irrigation increased biomass partitioning to fine roots and decreased partitioning to foliage, relative to other plant parts. Fertilization increased partitioning to perennial tissues (coarse roots, taproots, and branches) and decreased partitioning to ephemeral tissues (foliage and fine roots). Changes in allometry were small (< 6 %) but statistically significant, indicating that biomass partitioning in loblolly pine changes with altered resource availability, but is probably under strong ontogenetic control.