Two commercially available (Abbott Labs.) radioimmunoassays for determination of conjugated cholic acid and sulfoglycolithocholic acid in serum have been modified for bile acid measurements in alcoholic tissue extracts, bile, and urine. The specificity of both radioimmunoassays has been determined with regard to 27 free and conjugated bile acids. After filtration, bile acids can be measured in urine and bile without prior extraction. Tissue is homogenized and the bile acids are extracted into methanol. Urinary excretion by 64 healthy humans was 2.09 (SD 1.09) mumol of conjugated cholic acid and 8.44 (SD 8.03) mumol of sulfated glycolithocholic acid per 24 h. In liver from 10 patients with various noncholestatic liver disease, the mean concentration of conjugated cholic acid was 32.4 (SD 15.9) nmol/g wet weight. In the liver of 27 male Wistar rats, the concentration of conjugated cholic acid was 41.3 (SD 11.7) nmol/g of tissue, of sulfoglycolithocholic acid 5.1 (SD 2.3) nmol/g of tissue.
d g r u b e , W . E r b und E . B o h l e * *Die Auftrennung von Serumlipiden durch diinnschichtdwom a t o g r a p h i d e Verfahren laDt sich d u r d mehrere positive Momente begriinden: 1. Der apparative Aufwand ist relativ einfa&, 2. die Ergebnisse sind gut reproduzierbar, 3. die einzusetzenden Lipidmengen konnen gering gehalten werden, 4. mit den genannten Verfahren lassen sich durch einen Arbeitsgang alle a&t Lipidfraktionen gleichzeitig darstellen, 5. dementsprehend konnen definierte Lipidgruppen genauer als mit den Gruppenreaktionen der gebrauchlidwn &emis&-analytischen Verfahren bestimmt werden. 6 I. Folch, M . Lees u. G. H . Sloane Stanley, T. Lipid Res. 1, ---391 [1959]. i H . J . Wildgrube u. B. W o l f , im Druck. 8 0. S. Privett, M . L. Blank u. W . 0. Lundberg, .J. Amer. --Oil Chemists' S O~. 38, 312 [1961].
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