Measurement of conjugated bile acids by ion-pair high-performance liquid chromatography

Wildgrube, H. J.; Füssel, Uwe; Lauer, Hans–Christoph; Stockhausen, H.

doi:10.1016/s0021-9673(00)91637-6

Cited by 28 publications

(6 citation statements)

References 18 publications

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“…Initially, total bile acid (TBA) concentrations were measured using the 3a-hydroxysteroid dehydrogenase enzymatic procedure [ 141 and biliary bile acid composition was analysed by reverse ion-pair high-performance liquid chromatography technique (HPLC) [15]. To improve the sensitivity for bile acid detection, total and individual bile acid concentrations were measured by column gas chromatography (GC) [16, 171 using nordeoxycholic acid and coprostanol as internal standards.…”

Section: Laboratory Methodsmentioning

confidence: 99%

Bile acids analysis: a tool to assess graft function in human liver transplantation

et al. 2004

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The expanding use of “sub‐optimal” grafts due to donor organ shortage increases the importance of accurate graft assessment before liver transplantation. Bile secretion is an early sign of recovering hepatic function post‐transplant. The role of bile acid analysis in assessing graft function before and immediately after liver transplantation has been investigated. Two hundred and sixteen samples of hepatic bile were collected from 35 donors and 13 recipients. Clinical data, bile flow, total bile acid concentration, apparent choleretic activity and bile acid composition were assessed. Sub‐optimal donor livers showed a low apparent choleretic activity and a different bile acid composition when compared to normal grafts. In recipients, the pattern of recovery of bile secretion immediately after reperfusion was a useful predictor of graft function. This study characterises bile acid secretion of liver grafts and remarks the potential value of bile acid analysis to assess donor liver quality and early post‐transplant graft function.

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Section: Laboratory Methodsmentioning

confidence: 99%

Bile acids analysis: a tool to assess graft function in human liver transplantation

et al. 2004

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“…As part of a previous study by our group, 3 total biliary bile acids, 21 phospholipids 22 and cholesterol 23 were determined by standard enzymatic assays, and the cholesterol saturation index was derived 24 . The concentrations of the individual bile acid conjugates were determined using reverse‐phase high‐performance liquid chromatography, 25 and expressed as a percentage of total bile acids. Some of these data have been presented previously, 3 and were used in the present study to look for correlations with biliary phosphatidylcholine species and mucin glycoprotein concentrations (see below).…”

Section: Methodsmentioning

confidence: 99%

Octreotide increases the proportions of arachidonic acid‐rich phospholipids in gall‐bladder bile

Pereira

Hussaini

Murphy

et al. 2001

Aliment Pharmacol Ther

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Background and aims: Octreotide treatment of acromegalic patients induces cholesterol gallstone formation, in part by impairing cholecystokinin release and gall‐bladder contraction. However, there are few data on the effect of octreotide on biliary arachidonic acid‐rich phospholipids or mucin glycoprotein, factors which also influence cholesterol gallstone formation. Methods: In acromegalic patients studied before and during 3 months of octreotide treatment, we measured mucin glycoprotein concentrations and the molecular species of phosphatidylcholine, and related the results to the cholesterol saturation and percentage of deoxycholic acid in gall‐bladder bile. Results: The relative proportions of the major arachidonic acid‐rich phosphatidylcholine species, PC 16:0–20:4 and PC 18:0–20:4, increased significantly during octreotide treatment. These changes were associated with a rise in the cholesterol saturation index and a non‐significant twofold increase in mucin glycoprotein concentration. There were significant correlations between PC 16:0–20:4 and the cholesterol saturation index, percentage of vesicular cholesterol and percentage of deoxycholic acid in gall‐bladder bile. Conclusions: In acromegalic patients, octreotide increases the proportions of arachidonic acid‐rich phospholipids, with associated rises in: (a) the cholesterol saturation index and percentage of vesicular cholesterol, and (b) the percentage of deoxycholic acid in gall‐bladder bile—changes similar to those found in patients with cholesterol‐rich gall‐bladder stones.

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“…In 16 of the 23 patients with Crohn's disease, sufficient bile was obtained to assess both bile acid and phosphatidylcholine (the principal biliary phospholipid) fatty acid composition. The concentrations of the individual bile acid conjugates were determined using reverse‐phase high‐performance liquid chromatography, 19 and expressed as percentages of total conjugated bile acids. Following Fölch extraction of the lipid phase of gall‐bladder bile, phosphatidylcholine was separated from the other biliary lipids by high‐performance liquid chromatography, and the molecular species of the separated phosphatidylcholine were determined by reverse phase high‐performance liquid chromatography 13 .…”

Section: Bile Acid Conjugates and Phosphatidylcholine Speciesmentioning

confidence: 99%

Bile composition in inflammatory bowel disease: ileal disease and colectomy, but not colitis, induce lithogenic bile

Pereira

Bain

Kumar

et al. 2003

Aliment Pharmacol Ther

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SUMMARYBackground: Inflammatory bowel disease is a risk factor for gall-bladder stones, but there is controversy about the composition of these stones and whether such patients develop lithogenic bile. Methods: In 54 gallstone-free inflammatory bowel disease patients and 13 non-inflammatory bowel disease patients with cholesterol-rich gallstones, we measured the biliary cholesterol saturation indices, nucleation times and bilirubin concentrations, and determined the bile acid composition and molecular species of phosphatidylcholine, in gall-bladder bile. Results: Patients with Crohn's colitis or ulcerative colitis had less saturated bile (mean cholesterol saturation index, 0.9) and longer nucleation times (median,

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Measurement of conjugated bile acids by ion-pair high-performance liquid chromatography

Cited by 28 publications

References 18 publications

Bile acids analysis: a tool to assess graft function in human liver transplantation

Bile acids analysis: a tool to assess graft function in human liver transplantation

Octreotide increases the proportions of arachidonic acid‐rich phospholipids in gall‐bladder bile

Bile composition in inflammatory bowel disease: ileal disease and colectomy, but not colitis, induce lithogenic bile

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