Radioimmunoassay of bile acids in tissue, bile, and urine.

Wildgrube, H. J.; Stockhausen, H.; Metz, Peter; Mauritz, Gert-Jan; Mahdawi, R

doi:10.1093/clinchem/29.3.494

Cited by 12 publications

(2 citation statements)

References 13 publications

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“…Therefore, the measurement of bile acids in various compartments and systemic delineation of the changes of bile acids in whole gastrointestinal tracts, tissue, and circulation are very important for the full understanding of biological functions of bile acids. To date, numerous reports have been analyzed bile acids in various biological matrices , such as chemical , thin layer chromatography , HPLC , radioimmunoassay , enzyme linked colorometric and radioimmunoassay , MS and direct infusion ESI‐MS , MS/MS , GC using high resolution glass capillary columns and MS , GC , GC‐MS , enzymatic colorimetric method and enzymatic fluorimetric method , NMR spectroscopy , LC coupled with ultraviolet (UV) or MS , HPLC‐MS/MS , HPLC‐ELSD , and ultra‐performance LC‐MS detection . NMR methods are relatively insensitive and only applicable in specific areas where the concentration of bile acids is very high, such as the analysis of bile composition .…”

Section: Characterizing Metabolic Interactions Between Microbe and Hostmentioning

confidence: 99%

Metabolomics approaches for characterizing metabolic interactions between host and its commensal microbes

et al. 2013

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It is increasingly evident that the gut microbiota is involved in the regulation of multiple mammalian metabolic pathways through a series of interactive host-microbiota metabolic, signaling, and immune-inflammatory axes that physiologically connect the gut, liver, brain, and other organs. Correlation of the metabotypes with the gut microbial profiles derived from culture-independent molecular techniques is increasingly useful for deciphering inherent and intimate host-microbe relationships. Real-time analysis of the small molecule metabolites derived from gut microbial-host co-metabolism is essential for understanding the metabolic functions of the gut microbiome and has tremendous implications for personalized healthcare strategies. Metabolomics, an array of analytical techniques that includes high resolution NMR spectroscopy and chromatography-MS in conjunction with chemometrics and bioinformatics tools, enables characterization of the metabolic footprints of mammalian hosts that correlate with the microbial community in the intestinal tract. The metabolomics approach provides important information of a complete spectrum of metabolites produced from the gut microbial-mammalian co-metabolism and is improving our understanding of the molecular mechanisms underlying multilevel host-microbe interactions. In this review, the interactions of gut microbiota with their host are discussed and some examples of NMR- or MS-based metabolomics applications for characterizing the metabolic footprints of gut microbial-host co-metabolism are described. Advances in the metabolomic analysis of bile acids, short-chain fatty acids, and choline metabolism are also summarized.

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Section: Characterizing Metabolic Interactions Between Microbe and Hostmentioning

confidence: 99%

Metabolomics approaches for characterizing metabolic interactions between host and its commensal microbes

et al. 2013

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“…Διατίθενται, εμπορικά σε τυποποιημένη συσκευασία τα κατάλληλα αντιδραστήρια για τον προσδιορισμό του θειοεστέρα του γ λυκολιθοχολικού οξέος, του γλυκοχολικού οξέος και των παραγώγων των κυριωτέρων χολικών οξέων (135). Με τη μέθοδο αυτή μπορούν να προσδιοριστούν τα χολικά οξέα στον ηπατικό ιστό, σε ούρα και χολή (136), καβώς και στον ορό (137). Η μεγάλη δυσκολία παρασκευής των εξειδικευμένων αντιδραστηρίων, που απαιτούνται στις μεθόδους αυτές, είναι σχεδόν απαγορευτική για την ευρύτερη εφαρμογή προτεινόμενων μη εμπορικά αξιοποιημένων μεθόδων, όπως π.χ.…”

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Κινητικη Μελετη Και Αναλυτικες Εφαρμογες Ενζυμικων Συστηματων Για Τον Προσδιορισμο Χολικων Οξεων Σε Βιολογικα Υγρα

Λιανιδου¹

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Predictive value of random sample urine bile acids corrected by creatinine in liver disease

1987

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Bile acids, in a random sample of urine, discriminated normal controls from liver disease, with a probability similar to fasting plasma bile acids (p less than 0.01 and p less than 0.001, depending on the analytical technique). A high degree of correlation between urinary and plasma bile acids (up to r = 0.93) was achieved only when the urine flow was corrected by using a urinary bile acids/creatinine ratio but not with urinary bile acids as simple volume concentration. These findings originated from 10 patients with severe liver disease and 10 with mild liver disease, all biopsy-confirmed, compared with controls. In 24 normal controls and 24 histologically confirmed compensated liver disease, the predictive value of urinary bile acids/creatinine equaled or exceeded fasting plasma bile acids and routine liver tests. In a patient recovering from subacute hepatic necrosis, the urinary bile acids/creatinine closely resembled changes in plasma bile acids and in the routine liver tests. When sulfated urinary bile acids were included, the discrimination between liver disease and controls did not improve. Gallbladder contraction induced by parenteral analogs of cholecystokinin did not change urinary bile acids/creatinine, despite a significant increase in the plasma bile acids. Collection of fasting urine is thus not necessary. Urinary bile acids/creatinine in 12 subjects with renal insufficiency and moderate impairment of creatinine clearance was not different from controls. The weight/height index did not affect this urinary test: there was no significant correlation between the two. Available radioimmunoassays for plasma bile acids can be easily adapted for urine.(ABSTRACT TRUNCATED AT 250 WORDS)

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Radioimmunoassay of bile acids in tissue, bile, and urine.

Cited by 12 publications

References 13 publications

Metabolomics approaches for characterizing metabolic interactions between host and its commensal microbes

Metabolomics approaches for characterizing metabolic interactions between host and its commensal microbes

Κινητικη Μελετη Και Αναλυτικες Εφαρμογες Ενζυμικων Συστηματων Για Τον Προσδιορισμο Χολικων Οξεων Σε Βιολογικα Υγρα

Predictive value of random sample urine bile acids corrected by creatinine in liver disease

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