Naturally occurring conjugated bile acids, measured by high-performance liquid chromatography, in human, dog, and rabbit bile

Wildgrube, H. J.; Stockhausen, H.; Petri, J.; Füssel, Uwe; Lauer, Hans–Christoph

doi:10.1016/s0021-9673(01)87090-4

Cited by 36 publications

(21 citation statements)

References 13 publications

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“…Previously applied invasive cannulation methods of the duodenum (Wildgrube et al, 1986), the common biliary duct (Abedin et al, 1989), or the gallbladder (Kelly et al, 1993) are not only complicated but can influence hepatobiliary function as well.…”

Section: Acta Veterinaria Hungarica 50 2002mentioning

confidence: 99%

“…Changes in the composition of bile and its relationship with the pathogenesis of gallstone formation is an interesting field of clinical research (Wildgrube et al, 1986;Kelly et al, 1993;Tudyka et al, 1994;Washizu et al, 1994;Masamune et al, 1997).…”

mentioning

confidence: 99%

“…Bile from the gallbladder of human patients can be gained intraoperatively during surgical treatment of biliary disorders (Wildgrube et al, 1986;Armstrong et al, 2000) or, more conveniently, by ultrasound-guided percutaneous cholecystocentesis (Swobodnik et al, 1991;vanSonnenberg et al, 1992;Tudyka et al, 1995). The latter method has been described in the owl monkey (Pekow et al, 1994), in swine (Klapdor et al, 1997;Kessler et al, 2001) as well as in cattle (Braun and Gerber, 1992;Braun et al, 1995).…”

mentioning

confidence: 99%

“…The latter method has been described in the owl monkey (Pekow et al, 1994), in swine (Klapdor et al, 1997;Kessler et al, 2001) as well as in cattle (Braun and Gerber, 1992;Braun et al, 1995). In the dog, bile from the gallbladder was traditionally gained after surgical cannulation of the duodenum (Wildgrube et al, 1986), the common biliary duct (Abedin et al, 1989) or the gallbladder (Kelly et al, 1993), or by cholecystocentesis during laparotomy (Washizu et al, 1990;Masamune et al, 1997).…”

mentioning

confidence: 99%

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Percutaneous ultrasound-guided cholecystocentesis in dogs

Vörös

Sterczer

Manczur

et al. 2002

Acta Veterinaria Hungarica

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Percutaneous ultrasound-guided cholecystocentesis was performed on 13 healthy beagle dogs to determine whether percutaneous ultrasound-guided cholecystocentesis in the dog was a feasible and safe procedure. Clinical, laboratory and ultrasonographic examinations were done at 0 and 10 minutes, in the 2nd and 16th hour, and on the 7th day. They included a detailed physical examination of the mucous membranes, cardiorespiratory system and abdominal organs. Laboratory examinations of the blood consisted of a complete blood count, determination of packed cell volume (PCV), haemoglobin (Hb), total plasma protein (TPP), parameters of haemostasis including prothrombin time (PT), activated partial thromboplastin time (APTT), and enzyme activities reflecting hepatobiliary function, i.e. aspartate aminotransferase (AST), alanine aminotransferase (ALT), and gamma-glutamyltransferase (GGT). Ultrasonographic findings of the gallbladder (size, shape, wall, content) and appearance of the biliary tract and the surrounding cranial intraabdominal organs were also evaluated. Percutaneous ultrasoundguided cholecystocentesis was performed easily during the study, and dogs tolerated well the procedure performed without anaesthesia. All laboratory parameters of the blood remained within normal limits throughout the study. However, some follow-up values, i.e. PCV, TPP, APTT and ALT, demonstrated statistically significant differences when compared to baseline measurements, which might reflect the effect of 24-hour fasting before the experiment, as well as day-to-day metabolic fluctuations due to feeding and water supply during the study. There were no visible signs of bleeding from the liver, bile leakage from the gallbladder or accumulation of free peritoneal fluid during repeated ultrasonographic examinations. Percutaneous ultrasound-guided cholecystocentesis seems to be an important diagnostic procedure in canine gallbladder diseases and can be used safely and easily to gain gallbladder bile for diagnosis of bacterial cholecystitis or for investigating hepatobiliary function in the dog.

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Section: Acta Veterinaria Hungarica 50 2002mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Percutaneous ultrasound-guided cholecystocentesis in dogs

Vörös

Sterczer

Manczur

et al. 2002

Acta Veterinaria Hungarica

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“…Due to the extremely low aqueous solubility of cholesterol and oxysterols, in vitro studies require dissolution of these sterols in model bile or organic solvents. We selected the more physiological option, model bile, which was designed to mimic the lipid composition and concentrations of dog gallbladder bile in which taurocholate, the least toxic primary bile acid in mammalian bile, constitutes ϳ75% of the bile salts (55). Composition of the model bile approximated hepatic bile as to the concentration of its components.…”

Section: Discussionmentioning

confidence: 99%

Oxysterols from human bile induce apoptosis of canine gallbladder epithelial cells in monolayer culture

Seo

Choi

Lee

et al. 2004

American Journal of Physiology-Gastrointestinal and Liver Physi

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Oxysterols have been detected in various mammalian organs and blood. Biliary epithelium is exposed to high concentrations of cholesterol, and we have identified three keto-oxysterols (cholest-4-en-3-one, cholesta-4,6-dien-3-one, cholesta-3,5-dien-7-one) in human bile and gallstones. Because the effects of oxysterols on biliary physiology are not well defined, we investigated their biological effects on dog gallbladder epithelial cells. Enriched medium (culture medium containing taurocholate and lecithin and cholesterol +/- various oxysterols) was applied to confluent monolayers of dog gallbladder epithelial cells in culture. Cytotoxicity and apoptosis were studied by morphological analysis and flow cytometry. Oxysterols in the mitochondrial fraction were identified by gas chromatography/mass spectrometry, whereas release of cytochrome c from mitochondria was assayed by spectrophotometry and Western blot analysis. Compared with cells treated with culture medium or with enriched medium containing cholesterol, oxysterol-treated cells showed significantly increased apoptosis (P < 0.05). Exogenously applied oxysterols were recovered from the mitochondrial fraction. Cytochrome c release from mitochondria was increased significantly by cholest-4-en-3-one, cholesta-4,6-dien-3-one, and 5beta-cholestan-3-one (all P < 0.05). Thus oxysterols recovered from human bile and gallstones induce apoptosis of biliary epithelium via a mitochondrial-dependent pathway and may play a role in the pathogenesis of chronic inflammation and carcinogenesis in the gallbladder.

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