Simple, low-cost, and sensitive new platforms for electrochemical immunosensors for virus detection have been attracted attention due to the recent pandemic caused by a new type of coronavirus (SARS-CoV-2). In the present work, we report for the first time the construction of an immunosensor using a commercial 3D conductive filament of carbon black and polylactic acid (PLA) to detect Hantavirus Araucaria nucleoprotein (Np) as a proof-of-concept. The recognition biomolecule was anchored directly at the filament surface by using N -(3-Dimethylaminopropyl)- N ′-ethylcarbodiimide hydrochloride and N -Hydroxysuccinimide (EDC/NHS). Conductive and non-conductive composites of PLA were characterized using thermal gravimetric analysis (TGA), revealing around 30% w/w of carbon in the filament. Morphological features of composites were obtained from SEM and TEM measurements. FTIR measurement revealed that crosslinking agents were covalently bonded at the filament surface. Electrochemical techniques such as cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used for the evaluation of each step involved in the construction of the proposed immunosensor. The results showed the potentiality of the device for the quantitative detection of Hantavirus Araucaria nucleoprotein (Np) from 30 μg mL −1 to 240 μg mL −1 with a limit of detection of 22 μg mL −1 . Also, the proposed immunosensor was applied with success for virus detection in 100x diluted human serum samples. Therefore, the PLA conductive filament with carbon black is a simple and excellent platform for immunosensing, which offers naturally carboxylic groups able to anchor covalently biomolecules.

-This study compared different strategies to improve occupational health and evaluated their impact on some quality of life domains (health, physical activity, occupational environment, and perception of quality of life). This cluster randomized controlled clinical trial enrolled 172 men and women aged 26.10 ± 6.03 who worked in the administrative department of 4 companies. The interventions lasted three months. Employees in company A (EA) received workplace exercises (WE) and educational interventions (EI) (posters with healthcare and quality of life recommendations and computer messages); in Company B (EB), only WE; in company C (EC), only EI; and in company D (ED), employees formed a control group. A quality of life and health (QVS-80) questionnaire was used for all assessments. The Wilcoxon test was used to compare quality of life before and after interventions, and the Kruskal-Wallis test (p ≤ 0.05), for each quality of life domain. The scores for occupational environment increased in companies A and B (p <0.01), which suggests that WE may improve the occupational environment. Key words: Health promotion; Occupational health; Physical activity; Quality of life. Resumo -O objetivo do presente estudo foi comparar diferentes intervenções de promoção à saúde do trabalhador e seu impacto nos domínios da qualidade de vida (Saúde, Atividade Física, Ambiente Ocupacional e Percepção da Qualidade de Vida). Foi conduzido um ensaio clínico controlado randomizado por cluster em 4 empresas com 172 trabalhadores de ambos os sexos, com idade média de 26,10 ± 6,03, todos do setor administrativo. As intervenções tiveram duração de três meses. A Empresa A (EA) recebeu o exercício físico no local de trabalho [ginástica laboral (GL)] mais as intervenções educativas (IE: cartazes com recomendações de saúde e qualidade de vida e software computacional); a Empresa B (EB) recebeu a GL; os trabalhadores da Empresa C (EC) receberam a IE; a Empresa D (ED) foi o controle. As avaliações da qualidade de vida no trabalho ocorreram por meio do Questionário de Avaliação da Qualidade de Vida e Saúde (QVS-80 28Comparison of worker's health promotion interventions Grande et al.

Introduction: About one-third of the day goes on in the workplace. Therefore, strategies that benefit the quality of life of workers become important. Objective: To investigate determinants of quality of life after three months of workers' health promotion programs. Methods: An experimental design was used to verify the quality of life outcomes of 190 workers. The interventions lasted three months. Company A received the exercise program, posters with recommendations on health and quality of life and computer software; Company B received only an exercise program; Company C received posters with recommendations on health and quality of life and computer software, Company D was the control. All assessments of the quality of work life occurred through the questionnaire QVS-80. Data were analyzed through descriptive statistics, the z test and Cronbach's alpha test. results: The main factors that interfered in the quality of life were: physical activity focused on aesthetics, physical fitness, smoking, physical activity recommended by a doctor, sitting time, family life, sleep quality, income. Comparing national data to the present study for all chronic diseases self-reported, statistically significant differences were observed. Physical activity for aesthetic reasons is the variable that most negatively influences on the perception of quality of life. Conclusion: These data help to reflect on the importance of combined strategies such as physical activity implementation and the understanding on the lifestyle components in the workplace.

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The COVID-19 virus had its origin in the city of Whuan - China. This virus has a clinical picture ranging from asymptomatic infections to severe respiratory conditions. Through this virus it was decreed by the World Health Organization as being a pandemic, with it routine aspects were compromised, causing new solutions to be proposed. Quality of life was one of the factors affected by this virus, especially when we relate to teachers, as they had to adapt to new teaching methods, moving from classroom to virtual teaching. This study aimed to assess the perception of quality of life of teachers from public and private schools through the COVID-19 pandemic. The sample consisted of 35 volunteers, being teachers from public and private schools, of both sexes. As a research instrument, the WHOQOL-BRIEF - an instrument that proposes to evaluate the Quality of Life, applied in a virtual way through the Google Forms platform, is composed of 26 questions subdivided into four domains: Domain I - Physical; Domain II - Psychological; Domain III - Social Relationships and Domain IV - Environment. Descriptive statistics for the results were performed. The level of significance adopted for this study was 5% (P≤0.05). The results found show the physical domain with 70.71 points; psychological domain 68.21 points; social domain 64.52 points and environment domain 64.91. It is concluded that the teachers evaluated in most part have suffered with the aspect involving the quality of life.